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Techniques for Arbuscular Mycorrhiza Inoculum Reduction

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Symbiotic Fungi

Part of the book series: Soil Biology ((SOILBIOL,volume 18))

Abstract

Given the ubiquous presence of AM fungi, a major constraint to the evaluation of the activity of AM colonization has been the need to account for the indigenous soil native inoculum. This has to be controlled (i.e., reduced or eliminated) if we are to obtain a true control treatment for analysis of arbuscular mycorrhizas in natural substrates. There are various procedures possible for achieving such an objective, and the purpose of this chapter is to provide details of a number of techniques and present some evaluation of their advantages and disadvantages.

Soil solarization, steam sterilization, pasteurization, γ irradiation, chemicals application, soil disturbance, crop rotation, the use of ozone and myc− plants are the techniques focused. These techniques differently affect physical and chemical soil characteristics, as well as eliminating soil microorganisms that can interfere with the development of mycorrhizas.

No method developed for reducing the competition from indigenous AM fungi is ideal for every application, and the ultimate choice of methodology for establishing an adequate non-mycorrhizal control depends on the design of the particular experiments, the facilities available and the amount of soil requiring treatment.

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References

  • Abbott LK, Robson AD (1991) Field management of VA mycorrhizal fungi. In: Keister DL, Gregan PB (eds) The rhizosphere and plant growth. Kluwer, Netherlands, pp 355–362

    Google Scholar 

  • Afek U, Menge JA, Johnson ELV (1991) Interaction among mycorrhizae, soil solarization, metalaxyl, and plants in the field. Plant Dis 75:665–672

    CAS  Google Scholar 

  • Al-Momani A, Abugarbiah W, Saleh H (1988) The effect of soil solarization on endomycorrhizal fungi (Glomus mosseae) and Fusarium fungi. Dirasat 15:85–95

    Google Scholar 

  • Alphei J, Scheu S (1993) Effects of biocidal treatments on biological and nutritional properties of a mull-structured woodland soil. Geoderma 56:435–448

    Article  CAS  Google Scholar 

  • Antunes PM, Deaville D, Goss MJ (2006) Effect of two AMF life strategies on the tripartite symbiosis with Bradyrhizobium japonicum and soybean. Mycorrhiza 16:167–173

    Article  PubMed  Google Scholar 

  • Arihara J, Karasawa T (2000) Effect of previous crops on arbuscular mycorrhizal formation and growth of succeeding maize. Soil Sci Plant Nutr 46:43–51

    Google Scholar 

  • Aryabod S, Fotovat A, Lakzian A, Haghnia G (2006) The Effect of Soil Sterilization on Zn, Cu, Fe, and Mn Uptake by Maize. Abs 961b, 18th World Congress of Soil Science, Philadelphia, PA, USA

    Google Scholar 

  • Baptista MJ, Souza RB, Pereira W, Carijo AO, Vidal MC, Charchar JM (2006) Solarização do solo e biofumigação no cultivo protegido do tomate. Hortic Bras 4:47–52

    Google Scholar 

  • Bell CH (2000) Fumigation in the 21st century. Crop Prot 19:563–569

    Article  CAS  Google Scholar 

  • Bendavid-Val R, Babinowitch HD, Katan J, Kapulnik Y (1997) Viability of VA-mycorrhizal fungi following soil solarization and fumigation. Plant Soil 195:185–193

    Article  CAS  Google Scholar 

  • Blanke V, Renker C, Wagner M, Füllner K, Held M, Kuhn AJ, Buscot F (2005) Nitrogen supply affects arbuscular mycorrhizal colonization of Artemisia vulgaris L. in a phosphate-polluted field site. New Phytol 166:981–992

    Article  CAS  PubMed  Google Scholar 

  • Bowen GD, Rovira AD (1961) Plant growth in irradiated soil. Nature 191:936–937

    Article  Google Scholar 

  • Brito I, Carvalho M, van Tuinen D, Goss MJ (2006) Effects of soil management on arbuscular mycorrhizal fungi in autumn-sown crops in Mediterranean climates. In: Horn R, Fleige H, Peth S, Peng X (eds) Soil Management for sustainability, Advances in geoecology 38. Catena Verlag GmbH, Germany, pp 149–156

    Google Scholar 

  • Covacevich F, Echeverria HE (2003) Utilización de formaldehido para la erradicación de hongos micorríticos arbusculares de muestras de suelo. Ciencia del Suelo 21:9–17

    Google Scholar 

  • Egli M, Mirabella A, Kägi B, Tomasone G, Clorio G (2006) Influence of steam sterilization on soil chemical characteristics, trace metals and clay mineralogy. Geoderma 131:123–142

    Article  CAS  Google Scholar 

  • Endlweber K, Scheu S (2006) Establishing arbuscular mycorrhiza-free soil: a comparison of six methods and their effects on nutrient mobilization. Appl Soil Ecol 34:276–279

    Article  Google Scholar 

  • Evans DG, Miller MH (1990) The role of the external mycelial network in the effect of soil disturbance upon vesicular–arbuscular mycorrhizal colonization of maize. New Phytol 114:65–71

    Article  Google Scholar 

  • Fairchild GL, Miller MH (1988) Vesicular–arbuscular mycorrhizas and the soil-disturbance-induced reduction of nutrient absorption in maize. II. Development of the effect. New Phytol 110:75–84

    Article  Google Scholar 

  • Gantotti BV, Rangaswami G (1971) Improvements in the physical properties of soil under the influence of the rhizosphere microflora of four different plant species. Plant Soil 35:347–356

    Article  Google Scholar 

  • Gollner M, Friedel JK, Freyer B (2004) Influence of different agricultural practices on the arbuscular mycorrhiza in organic farming systems. In: Baar J, Josten E (eds) Applied Plant Research, WUR, Netherlands: Role of mycorrhiza in sustainable land management. COST 8.38 Meeting, 26–27 February 2004, Vught, Netherlands

    Google Scholar 

  • Goss MJ, de Varennes A (2002) Soil disturbance reduces the efficacy of mycorrhizal associations for early soybean growth and N2 fixation. Soil Biol Bioch 34:1167–1173

    Article  CAS  Google Scholar 

  • Habte M, Manjunath A (1992) Initial and residual toxicity of soil-applied thiram on the vesicular–arbuscular mycorrhizal symbiosis. Mycorrhiza 2:25–31

    Article  CAS  Google Scholar 

  • Harinikumar KM, Bagyaraj DJ (1988) Effect of crop rotation on native vesicular–arbuscular mycorrhizal propagules in soil. Plant Soil 110:77–80

    Article  Google Scholar 

  • Kabir Z (2005) Tillage or no-tillage: Impact on mycorrhizae. Can J Plant Sci 85:23–29

    Google Scholar 

  • Kabir Z, O`Halloran IP, Widden P, Hamel C (1998) Vertical distribution of arbuscular mycorrhizal fungi under corn (Zea mays L.) in no-till and conventional tillage systems. Mycorrhiza 8:53–55

    Article  Google Scholar 

  • Kahiluoto H, Ketoja E, Vestberg M (2000) Creation of a non-mycorrhizal control for a biossay of AM effectiveness. Mycorrhiza 9:241–258

    Article  Google Scholar 

  • Karasawa T, Kasahara Y, Takebe M (2001) Variable response of growth and arbuscular mycorrhizal colonization of maize plants to preceding crops in various types of soils. Biol Fertil Soils 33:286–293

    Article  CAS  Google Scholar 

  • Klironomos JN, Hart MM (2002) Colonization of roots by arbuscular mycorrhizal fungi using different sources of inoculum. Mycorrhiza 12:181–184

    Article  PubMed  Google Scholar 

  • Manjunath A, Bagyaraj DJ (1984) Effects of fungicides on mycorrhizal colonization and growth of onion. Plant Soil 80:147–150

    Article  CAS  Google Scholar 

  • Marsh JF, Schultze M (2001) Analysis of arbuscular mycorrhizas using symbiosis-defective plant mutants. New Phytol 150:525–532

    Article  Google Scholar 

  • Martins MA, Read DJ (1997) The effects of disturbance on the external mycelium of arbuscular mycorrhizal fungi on plant growth. Pesqui Agropecu Bras 32:1183–1189

    Google Scholar 

  • McGonigle TP, Miller MH (1996) Development of fungi below ground in association with plants growing in disturbed and undisturbed soils. Soil Biol Biochem 28:263–269

    Article  CAS  Google Scholar 

  • McGonigle TP, Yano K, Shinhama T (2003) Mycorrhizal phosphorus enhancement of plants in undisturbed soil differs from phosphorus uptake stimulation by arbuscular mycorrhizae over non-mycorrhizal controls. Biol Fertil Soil 37:268–273

    Google Scholar 

  • McLaren AD (1969) Radiation as a technique in soil biology and biochemistry. Soil Biol Bioch 1:63–73

    Article  CAS  Google Scholar 

  • McNamara NP, Black HIJ, Beresford NA, Parekh NR (2003) Effects of acute gamma irradiation on chemical, physical and biological properties of soils. Appl Soil Ecol 24:117–132

    Article  Google Scholar 

  • Menge JA (1982) Effect of soil fumigants and fungicides on vesicular–arbuscular fungi. Phytopathology 72:1125–1132

    Google Scholar 

  • Nair SK, Peethambaran CK, Geetha D, Nayar K, Wilson KI (1990) Effect of soil solarization on nodulation, infection by mycorrizal fungi and yield of cowpea. Plant Soil 125:153–154

    Article  Google Scholar 

  • Nakagawa LE, Andrea MM (1997) Liberação de resíduos ligados de atrazina de solos por meio de esterilização por autoclavagem e radiação gama. Rev Bras Ciênc Solo 21:707–710

    CAS  Google Scholar 

  • Parekh NR, Potter ED, Poskitt JS, Dodd BA, Beresford NA (2005) Detection of irradiation-induced changes on the activity and diversity of soil microbial communities: the effect of soil type. Radioprotection 40:S939–S944

    Article  Google Scholar 

  • Plenchette C, Fortin JA, Furlan V (1983) Growth response of several plants species to mycorrhiza in a soil of moderate P fertility. I. Mycorrhizal dependency under field conditions. Plant Soil 70:199–209

    Article  CAS  Google Scholar 

  • Powlson DS, Jenkinson DS (1976) The effects of biocidal treatments on metabolism in soil. II. Gamma irradiation, autoclaving, air-drying and fumigation. Soil Biol Bioch 8:179–188

    Article  CAS  Google Scholar 

  • Rose RE, Bailey RW (1952) Ethylene oxide for soil sterilisation. Nature 169:716

    Article  CAS  PubMed  Google Scholar 

  • Salem SF, Dobolyi C, Helyes L, Pçk Z, Dimçny J (2003) Side-effect of benomyl and captan on arbuscular mycorrhiza formation in tomato. Acta Hort 613:243–246

    CAS  Google Scholar 

  • Scagel CF (2004) Soil pasteurization and mycorrhizal inoculation alters flower production and corm composition of Brodiaea laxa. ‘Queen Fabiola’. Hort Sci 39:1432–1437

    Google Scholar 

  • Schreiner RP, Ivors KL, Pinkerton JN (2001) Soil solarization reduces arbuscular mycorrhizal fungi as a consequence of weed suppression. Mycorrhiza 11:273–277

    Article  CAS  Google Scholar 

  • Schreiner RP, Bethlenfalvay GJ (1997) Mycorrhizae, biocides, and biocontrol. 3. Effects of three different fungicides on developmental stages of three AM fungi. Biol Fertil Soils 24:18–26

    Article  CAS  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal Symbiosis. Academic, London

    Google Scholar 

  • Smith FA, Smith SE (1981) Mycorrhizal Infection and Growth of Trifolium subterraneum: Use of sterilized soil as a control treatment. New Phytol 88:299–309

    Article  CAS  Google Scholar 

  • Stapleton JJ (1990) Thermal inactivation of crop pests and pathogens and other soil changes caused by solarization. In: International Conference of Soil Solarization, Ammam. Proceedings. DAO, Rome, pp 37–47

    Google Scholar 

  • Takayama M, Ebihara K, Stryczewska H, Ikegami T, GyoutokuY, Kubo K, Tachibana M (2006) Ozone generation by dielectric barrier discharge for soil sterilization. Thin Solid Films 506–507:396–399

    Article  Google Scholar 

  • Thompson JP (1990) Soil sterilization methods to show VA-mycorrhizae aid P and Zn nutrition of wheat in vertisols. Soil Biol Bioch 22:229–240

    Article  CAS  Google Scholar 

  • Trevors JT (1996) Sterilization and inhibition of microbial activity in soil. J Microbiol Methods 26:53–59

    Article  CAS  Google Scholar 

  • Vosátka M (1995) Influence of inoculation with arbuscular mycorrhizal fungi on the growth and mycorrhizal infection of transplanted onion. Agric Ecosyst Environ 53:151–159

    Article  Google Scholar 

  • Wolf DC, Skipper HD (1994) Soil sterilization. In: Weaver RW, Angle JS, Bottomley PS, Bezdicek D, Smith S, Tabatabai A, Wollum A, Mickelson SH (eds) Methods of soil analysis, Part 2 — Microbiological and biochemical properties. Soil Society of America Inc. pp 41–51

    Google Scholar 

  • Yardin MR, Kennedy IR, Thies JE (2000) Development of high-quality carrier materials for field delivery of key microorganisms used as bio-fertilisers and bio-pesticides. Radiat Phys Chem 57:565–568

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Universidade de Évora, ICAM, Apartado 94, 7002 -s 554, Évora, Portugal

    Isabel Brito & Mário de Carvalho

  2. Kemptville College, University of Guelph, Kemptville, Ontario, K0G 1J0, Canada

    Michael J Goss

Authors
  1. Isabel Brito
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  2. Mário de Carvalho
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  3. Michael J Goss
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Correspondence to Isabel Brito .

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Editors and Affiliations

  1. Amity Institute of Microbial Sciences, Amity University Uttar Pradesh, Noida, 201303, India

    Ajit Varma  & Amit C. Kharkwal  & 

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Brito, I., de Carvalho, M., Goss, M.J. (2009). Techniques for Arbuscular Mycorrhiza Inoculum Reduction. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_19

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