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Functional Genomic of Arbuscular Mycorrhizal Symbiosis: Why and How Using Proteomics

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Symbiotic Fungi

Part of the book series: Soil Biology ((SOILBIOL,volume 18))

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Abstract

This chapter aims to provide a general technical overview of the contribution of proteomics to the identification of arbuscular mycorrhizal symbiosis-related proteins. Key features with regard to biological material handling; equipment, protein separation and detection protocols are first highlighted, before a case study of combined transcriptomic and proteomic investigations targeted at the early stages of this plant–mycorrhiza interaction is exposed.

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Abbreviations

AB :

Amersham biosciences

APS :

Ammonium persulfate

AM :

Arbuscular mycorrhiza

CHAPS :

3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propanesulfonate

DEPC :

diethylpyrocarbonate

DTT :

1,4-dithio-DL-threitol

2-DE :

Two-dimensional electrophoresis

ESI :

Electrospray ionization

ESTs :

Expressed sequence tags

IEF :

Isoelectrofocusing

IPGs :

Strips of immobilized pH gradients

MS :

Mass spectrometry

MS-MS :

Tandem mass spectrometry

MALDI-TOF :

Matrix assisted laser desorption/ionization-time of flight

NCBI :

National Center for Biotechnology Information

PDA :

Piperazine diacrylamide

PMSF :

Phenylmethanesulfonyl fluoride

SDS :

Sodium dodecyl sulfate

sqf :

Sufficient quantity for

TEMED :

N,N,N2,N2-Tetramethylethylenediamine

Tris :

Tris-[hydroxymethyl] aminomethane

US DOE :

US Department of Energy

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Acknowledgments

The authors are grateful to the Burgundy Region for supporting Nardjis Amiour during her postdoctoral stay in the UMR PME 1088, and for financial support to two CRB contracts. They also would like to thank S. Weidman and V. Gianinazzi-Pearson for the kind gift of protein samples from their RNA extractions. A special thank to JM Lamalle for help for constructing figures, and to Céline Henry (INRA, Jouy-en-Josas, France) who performed mass spectrometry analyses.

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Authors and Affiliations

  1. UMR 1088 INRA/CNRS 5184/UB Plante-Microbe-Environnement, INRA/CMSE, BP, 86510, 21065, Dijon Cedex, France

    Eliane Dumas-Gaudot, Ghislaine Recorbet & Franck Robert

  2. UMR de Génétique Végétale, IFR 87, Plate-Forme de Protéomique du Moulon, Gif sur Yvette, France

    Benoit Valot

  3. Unité de Nutrition Azoteé, IJPB, INRA Centre de Versailles, 78026, Versailles Cedex, France

    Nardjis Amiour

Authors
  1. Eliane Dumas-Gaudot
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  2. Ghislaine Recorbet
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  3. Franck Robert
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  4. Benoit Valot
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  5. Nardjis Amiour
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Corresponding author

Correspondence to Eliane Dumas-Gaudot .

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Editors and Affiliations

  1. Amity Institute of Microbial Sciences, Amity University Uttar Pradesh, Noida, 201303, India

    Ajit Varma  & Amit C. Kharkwal  & 

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Dumas-Gaudot, E., Recorbet, G., Robert, F., Valot, B., Amiour, N. (2009). Functional Genomic of Arbuscular Mycorrhizal Symbiosis: Why and How Using Proteomics. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_15

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