Abstract
Plant roots form a part of a multitude of organismic interactions within the soil. Due to the release of organic compounds they attract diversified microbiological populations where fungi and bacteria can form distinct communities. In this root sphere (“rhizosphere”), fungi can be extremely helpful for the plant in facilitating the supply of water and nutrients (mycorrhiza), but also detrimental, if they act as pathogens (e.g., root-rot fungi). Rhizosphere bacteria (e.g., streptomycetes) can fine-tune such interactions by releasing compounds which have biocontrol effects (support of symbiotic fungi/mycorrhization; growth inhibition of pathogenic fungi), or which increase the resistance of the plant to pathogens (priming effect). In this chapter, methods are presented which allow for the investigation of such interactions for up to three partners under in vitro conditions. These culture techniques form the basis for investigating the molecular and physiological impacts of microbial interactions with each other and with the plant, including a nondestructive test of plant vitality via chlorophyll fluorescence.
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Hampp, R., Tarkka, M.T. (2009). Interaction with Soil Microorganisms. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_12
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DOI: https://doi.org/10.1007/978-3-540-95894-9_12
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