Abstract
A simple, rapid and inexpensive method for the isolation of metabolically active arbuscules from plant root of an increased arbuscule-forming mutant of Lotus japonicus is developed. The method of arbuscule isolation with or without enzyme digestion is outlined in this article. Enzyme histochemical staining showed that the isolated arbuscules had succinate dehydrogenase, alkaline phosphatase and acid phosphatase activities. The enzymic activity of the arbuscules was not affected after the isolation process. The development of a simple, rapid and inexpensive method for the isolation of metabolically active arbuscules will be useful to clarify the biochemical processes occurring in nutrient exchange at the arbuscular interface.
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Solaiman, Z.M. (2009). Isolation of Metabolically Active Arbuscules and Intraradical Hyphae from Mycorrhizal Roots. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_11
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DOI: https://doi.org/10.1007/978-3-540-95894-9_11
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