Abstract
A key technique for protein analysis is the geometric alignment of 2D gel electrophoresis (2-DE) images. We introduce a new hybrid elastic registration approach for 2-DE images, which is based on analytic solutions of the Navier equation. With this approach cross-effects in elastic deformations can be handled, which is important for the registration of 2-DE images. We have successfully applied our approach to register 2-DE gel images of different levels of complexity and have performed a quantitative evaluation of the results. We have also performed a comparison with a previous hybrid elastic registration scheme.
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References
Berth M, Moser FM, Kolbe M, et al. The state of the art in the analysis of 2D gel eletcrophoresis images. Appl Microbiol Biotechnol. 2007;76(6):1223–43.
Zitova B, Flusser J. Image registration methods: a survey. Image Vis Comput. 2003;24:977–1000.
Efrat A, Hoffmann F, Kriegel K, et al. Geometric algorithms for the analysis of 2D-eletrophoresis gels. J Comp Biology. 2002;9(2):299–316.
Veeser S, Dunn MJ, Yang GZ. Multiresolution image registration for two-dimensional gel electrophoresis. Proteomics. 2001;1:856–70.
Rohr K, Cathier P, Wörz S. Elastic registration of electrophoresis images using intensity information and point landmarks. Pattern Recog. 2004;37(5):1035–48.
Rogers M, Graham J, Tonge RP. 2D Electrophoresis gel registration using point matching and local image-based refinement. Proc 15th British Machine Vision Conf. 2004.
Wang X, Feng DD. Hybrid registration for 2D gel protein images. Proc 3rd Asia-Pacific Bioinf Conf. 2005 Jan.
Wörz S, Rohr K. Hybrid spline-based elastic image registration using analytic solutions of the navier equation. Proc BVM. 2007; p. 151–5.
Kohlrausch J, Rohr K, Stiehl HS. A new class of elastic body splines for nonrigid registration of medical images. J Math Imaging Vis. 2005;23(3):253–80.
Thévenaz P, Ruttimann UE, Unser M. A pyramid approach to subpixel registration based on intensity. IEEE Trans Image Process. 1998;7(1):27–41.
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© 2008 Springer-Verlag Berlin Heidelberg
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Wörz, S., Winz, ML., Rohr, K. (2008). Geometric Alignment of 2D Gel Electrophoresis Images. In: Tolxdorff, T., Braun, J., Deserno, T.M., Horsch, A., Handels, H., Meinzer, HP. (eds) Bildverarbeitung für die Medizin 2008. Informatik aktuell. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-78640-5_20
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DOI: https://doi.org/10.1007/978-3-540-78640-5_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-78639-9
Online ISBN: 978-3-540-78640-5
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