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Protein Secretion in Bacterial Cells

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Bacterial Physiology

Approximately 20% of the proteins synthesized in a bacterial cell are transported outside of the cytoplasm. These proteins are localized either within the different compartments of the cell envelope or are released into the extracellular growth medium. Bacteria have evolved multiple pathways to secrete proteins across their cell envelopes. In this chapter, we examine similarities and differences among the several types of protein secretion pathways found in bacteria, as well as in archaea. Because the applied aspects of bacterial protein secretion play a vital role in biological and pharmaceutical industries, a more comprehensive understanding of the mechanisms underlying these secretion systems is essential to the advancement of present technologies.

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References

  • Althoff S, Selinger D, Wise JA (1994) Molecular evolution of SRP cycle components: functional implications. Nucleic Acids Res 22: 1933–1947

    Article  PubMed  CAS  Google Scholar 

  • Backert S, Meyer TF (2006) Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol 9: 207–217

    Article  PubMed  CAS  Google Scholar 

  • Berks BC, Sargent F, Palmer T (2000) The Tat protein export pathway. Mol Microbiol 35: 260–274

    Article  PubMed  CAS  Google Scholar 

  • Berthet FX, Rasmussen PB, Rosenkrands I, Andersen P, Gicquel B (1998) A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). Microbiology 144 (Pt 11): 3195–3203

    Article  PubMed  CAS  Google Scholar 

  • Bolhuis A, Broekhuizen CP, Sorokin A, van Roosmalen ML, Venema G, Bron S, Quax WJ, van Dijl JM (1998) SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins. J Biol Chem 273: 21217–21224

    Article  PubMed  CAS  Google Scholar 

  • Braun V, Hobbie S, Ondraczek R (1992) Serratia marcescens forms a new type of cytolysin. FEMS Microbiol Lett 79: 299–305

    Article  PubMed  CAS  Google Scholar 

  • Champion PA, Stanley SA, Champion MM, Brown EJ, Cox JS (2006) C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 313: 1632–1636

    Article  PubMed  CAS  Google Scholar 

  • Chen LM, Briones G, Donis RO, Galan JE (2006) Optimization of the delivery of heterologous proteins by the Salmonella enterica serovar Typhimurium type III secretion system for vaccine development. Infect Immun 74: 5826–5833

    Article  PubMed  CAS  Google Scholar 

  • Christie PJ (2004) Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems. Biochim Biophys Acta 1694: 219–234

    Article  PubMed  CAS  Google Scholar 

  • Cornelis GR (2002) Yersinia type III secretion: send in the effectors. J Cell Biol 158: 401–408

    Article  PubMed  CAS  Google Scholar 

  • Cotter SE, Surana NK, St Geme JW 3rd (2005) Trimeric autotransporters: a distinct subfamily of autotransporter proteins. Trends Microbiol 13: 199–205

    Article  PubMed  CAS  Google Scholar 

  • Coutte L, Antoine R, Drobecq H, Locht C, Jacob-Dubuisson F (2001) Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway. Embo J 20: 5040–5048

    Article  PubMed  CAS  Google Scholar 

  • Dalbey RE, Chen M (2004) Sec-translocase mediated membrane protein biogenesis. Biochim Biophys Acta 1694: 37–53

    Article  PubMed  CAS  Google Scholar 

  • de Keyzer J, van der Does C, Driessen AJ (2003) The bacterial translocase: a dynamic protein channel complex. Cell Mol Life Sci 60: 2034–2052

    Article  PubMed  CAS  Google Scholar 

  • Delepelaire P (2004) Type I secretion in gram-negative bacteria. Biochim Biophys Acta 1694: 149–161

    Article  PubMed  CAS  Google Scholar 

  • Digiuseppe Champion PA, Cox JS (2007) Protein secretion systems in Mycobacteria. Cell Microbiol 9: 1376–1384

    Article  PubMed  CAS  Google Scholar 

  • Dilks K, Rose RW, Hartmann E, Pohlschroder M (2003) Prokaryotic utilization of the twin-arginine translocation pathway: a genomic survey. J Bacteriol 185: 1478–1483

    Article  PubMed  CAS  Google Scholar 

  • Driessen AJ, Fekkes P, van der Wolk JP (1998) The Sec system. Curr Opin Microbiol 1: 216–222

    Article  PubMed  CAS  Google Scholar 

  • Dudley EG, Thomson NR, Parkhill J, Morin NP, Nataro JP (2006) Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol 61: 1267–1282

    Article  PubMed  CAS  Google Scholar 

  • Economou A (2002) Bacterial secretome: the assembly manual and operating instructions (Review). Mol Membr Biol 19: 159–169

    Article  PubMed  CAS  Google Scholar 

  • Fisher AC, Kim W, DeLisa MP (2006) Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway. Protein Sci 15: 449–458

    Article  PubMed  CAS  Google Scholar 

  • Galan JE, Wolf-Watz H (2006) Protein delivery into eukaryotic cells by type III secretion machines. Nature 444: 567–573

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Rodriguez FM, Schrammeijer B, Hooykaas PJ (2006) The Agrobacterium VirE3 effector protein: a potential plant transcriptional activator. Nucleic Acids Res 34: 6496–6504

    Article  PubMed  CAS  Google Scholar 

  • Gentle IE, Burri L, Lithgow T (2005) Molecular architecture and function of the Omp85 family of proteins. Mol Microbiol 58: 1216–1225

    Article  PubMed  CAS  Google Scholar 

  • Georgiou G, Segatori L (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr Opin Biotechnol 16: 538–545

    Article  PubMed  CAS  Google Scholar 

  • Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss R 3rd (1997) Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15: 29–34

    Article  PubMed  CAS  Google Scholar 

  • Gohlke U, Pullan L, McDevitt CA, Porcelli I, de Leeuw E, Palmer T, Saibil HR, Berks BC (2005) The TatA component of the twin-arginine protein transport system forms channel complexes of variable diameter. Proc Natl Acad Sci USA 102: 10482–10486

    Article  PubMed  CAS  Google Scholar 

  • Grass S, St Geme JW 3rd (2000) Maturation and secretion of the non-typable Haemophilus influenzae HMW1 adhesin: roles of the N-terminal and C-terminal domains. Mol Microbiol 36: 55–67

    Article  PubMed  CAS  Google Scholar 

  • Hatzixanthis K, Palmer T, Sargent F (2003) A subset of bacterial inner membrane proteins integrated by the twin-arginine translocase. Mol Microbiol 49: 1377–1390

    Article  PubMed  CAS  Google Scholar 

  • He SY, Nomura K, Whittam TS (2004) Type III protein secretion mechanism in mammalian and plant pathogens. Biochim Biophys Acta 1694: 181–206

    Article  PubMed  CAS  Google Scholar 

  • Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC, Ala’Aldeen D (2004) Type V protein secretion pathway: the autotransporter story. Microbiol Mol Biol Rev 68: 692–744

    Article  PubMed  CAS  Google Scholar 

  • Hodak H, Clantin B, Willery E, Villeret V, Locht C, Jacob-Dubuisson F (2006) Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate. Mol Microbiol 61: 368–382

    Article  PubMed  CAS  Google Scholar 

  • Holland IB, Schmitt L, Young J (2005) Type 1 protein secretion in bacteria, the ABC-transporter dependent pathway (review). Mol Membr Biol 22: 29–39

    Article  PubMed  CAS  Google Scholar 

  • Jacob-Dubuisson F, Fernandez R, Coutte L (2004) Protein secretion through autotransporter and two-partner pathways. Biochim Biophys Acta 1694: 235–257

    Article  PubMed  CAS  Google Scholar 

  • Johnson TL, Abendroth J, Hol WG, Sandkvist M (2006) Type II secretion: from structure to function. FEMS Microbiol Lett 255: 175–186

    Article  PubMed  CAS  Google Scholar 

  • Jongbloed JD, Antelmann H, Hecker M, Nijland R, Bron S, Airaksinen U, Pries F, Quax WJ, van Dijl JM, Braun PG (2002) Selective contribution of the twin-arginine translocation pathway to protein secretion in Bacillus subtilis. J Biol Chem 277: 44068–44078

    Article  PubMed  CAS  Google Scholar 

  • Koronakis V, Eswaran J, Hughes C (2004) Structure and function of TolC: the bacterial exit duct for proteins and drugs. Annu Rev Biochem 73: 467–489

    Article  PubMed  CAS  Google Scholar 

  • Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405: 914–919

    Article  PubMed  CAS  Google Scholar 

  • Kostakioti M, Newman CL, Thanassi DG, Stathopoulos C (2005) Mechanisms of protein export across the bacterial outer membrane. J Bacteriol 187: 4306–4314

    Article  PubMed  CAS  Google Scholar 

  • Kuehn MJ, Heuser J, Normark S, Hultgren SJ (1992) P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips. Nature 356: 252–255

    Article  PubMed  CAS  Google Scholar 

  • Lambert-Buisine C, Willery E, Locht C, Jacob-Dubuisson F (1998) N-terminal characterization of the Bordetella pertussis filamentous haemagglutinin. Mol Microbiol 28: 1283–1293

    Article  PubMed  CAS  Google Scholar 

  • Lee PA, Tullman-Ercek D, Georgiou G (2006) The bacterial twin-arginine translocation pathway. Annu Rev Microbiol 60: 373–395

    Article  PubMed  CAS  Google Scholar 

  • Li H, Qian L, Chen Z, Thibault D, Liu G, Liu T, Thanassi DG (2004) The outer membrane usher forms a twin-pore secretion complex. J Mol Biol 344: 1397–1407

    Article  PubMed  CAS  Google Scholar 

  • Luirink J, Sinning I (2004) SRP-mediated protein targeting: structure and function revisited. Biochim Biophys Acta 1694: 17–35

    PubMed  CAS  Google Scholar 

  • Maeshima H, Okuno E, Aimi T, Morinaga T, Itoh T (2001) An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA. FEBS Lett 507: 336–340

    Article  PubMed  CAS  Google Scholar 

  • Meli AC, Hodak H, Clantin B, Locht C, Molle G, Jacob-Dubuisson F, Saint N (2006) Channel properties of TpsB transporter FhaC point to two functional domains with a C-terminal protein-conducting pore. J Biol Chem 281: 158–166

    Article  PubMed  CAS  Google Scholar 

  • Meng G, Surana NK, St Geme JW 3rd, Waksman G (2006) Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter. Embo J 25: 2297–2304

    Article  PubMed  CAS  Google Scholar 

  • Merritt EA, Hol WG (1995) AB5 toxins. Curr Opin Struct Biol 5: 165–171

    Article  PubMed  CAS  Google Scholar 

  • Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312: 1526–1530

    Article  PubMed  CAS  Google Scholar 

  • Muller JP, Ozegowski J, Vettermann S, Swaving J, Van Wely KH, Driessen AJ (2000) Interaction of Bacillus subtilis CsaA with SecA and precursor proteins. Biochem J 348 Pt 2: 367–373

    Article  PubMed  CAS  Google Scholar 

  • Newman CL, Stathopoulos C (2004) Autotransporter and two-partner secretion: delivery of large-size virulence factors by gram-negative bacterial pathogens. Crit Rev Microbiol 30: 275–286

    Article  PubMed  CAS  Google Scholar 

  • Ng SY, Chaban B, VanDyke DJ, Jarrell KF (2007) Archaeal signal peptidases. Microbiology 153: 305–314

    Article  PubMed  CAS  Google Scholar 

  • Nouwen N, Ranson N, Saibil H, Wolpensinger B, Engel A, Ghazi A, Pugsley AP (1999) Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation. Proc Natl Acad Sci USA 96: 8173–8177

    Article  PubMed  CAS  Google Scholar 

  • Nouwen N, Stahlberg H, Pugsley AP, Engel A (2000) Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy. Embo J 19: 2229–2236

    Article  PubMed  CAS  Google Scholar 

  • Oliver DC, Huang G, Nodel E, Pleasance S, Fernandez RC (2003) A conserved region within the Bordetella pertussis autotransporter BrkA is necessary for folding of its passenger domain. Mol Microbiol 47: 1367–1383

    Article  PubMed  CAS  Google Scholar 

  • Oomen CJ, van Ulsen P, van Gelder P, Feijen M, Tommassen J, Gros P (2004) Structure of the translocator domain of a bacterial autotransporter. Embo J 23: 1257–1266

    Article  PubMed  CAS  Google Scholar 

  • Pallen MJ, Chaudhuri RR, Henderson IR (2003) Genomic analysis of secretion systems. Curr Opin Microbiol 6: 519–527

    Article  PubMed  CAS  Google Scholar 

  • Palmer T, Berks RBC (2007) The Tat protein export pathway. In: Ehrmann M (ed) The periplasm. ASM Press, Washington D.C., pp 16–29

    Google Scholar 

  • Park HS, Kayser KJ, Kwak JH, Kilbane JJ 2nd (2004) Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase. J Ind Microbiol Biotechnol 31: 189–197

    Article  PubMed  CAS  Google Scholar 

  • Pohlschroder M, Gimenez MI, Jarrell KF (2005) Protein transport in Archaea: Sec and twin arginine translocation pathways. Curr Opin Microbiol 8: 713–719

    Article  PubMed  CAS  Google Scholar 

  • Pugsley AP, Francetic O, Possot OM, Sauvonnet N, Hardie KR (1997) Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria—a review. Gene 192: 13–19

    Article  PubMed  CAS  Google Scholar 

  • Pukatzki S, Ma AT, Sturtevant D, Krastins B, Sarracino D, Nelson WC, Heidelberg JF, Mekalanos JJ (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci USA 103: 1528–1533

    Article  PubMed  CAS  Google Scholar 

  • Rachel R, Wyschkony I, Riehl S, Huber H (2002) The ultrastructure of Ignicoccus: evidence for a novel outer membrane and for intracellular vesicle budding in an archaeon. Archaea 1: 9–18

    Article  PubMed  CAS  Google Scholar 

  • Rao PS, Yamada Y, Tan YP, Leung KY (2004) Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol 53: 573–586

    Article  PubMed  CAS  Google Scholar 

  • Roberts JA, Marklund BI, Ilver D, Haslam D, Kaack MB, Baskin G, Louis M, Mollby R, Winberg J. Normark S (1994) The Gal(alpha 1–4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract. Proc Natl Acad Sci USA 91: 11889–11893

    Article  PubMed  CAS  Google Scholar 

  • Sanchez-Pulido L, Devos D, Genevrois S, Vicente M, Valencia A (2003) POTRA: a conserved domain in the FtsQ family and a class of beta-barrel outer membrane proteins. Trends Biochem Sci 28: 523–526

    Article  PubMed  CAS  Google Scholar 

  • Sandkvist M (2001) Type II secretion and pathogenesis. Infect Immun 69: 3523–3535

    Article  PubMed  CAS  Google Scholar 

  • Sauer FG, Remaut H, Hultgren SJ, Waksman G (2004) Fiber assembly by the chaperone-usher pathway. Biochim Biophys Acta 1694: 259–267

    Article  PubMed  CAS  Google Scholar 

  • Schindel C, Zitzer A, Schulte B, Gerhards A, Stanley P, Hughes C, Koronakis V, Bhakdi S, Palmer M (2001) Interaction of Escherichia coli hemolysin with biological membranes. A study using cysteine scanning mutagenesis. Eur J Biochem 268: 800–808

    Article  PubMed  CAS  Google Scholar 

  • Schlumberger MC, Hardt WD (2006) Salmonella type III secretion effectors: pulling the host cell’s strings. Curr Opin Microbiol 9: 46–54

    Article  PubMed  CAS  Google Scholar 

  • Sibbald MJ, Ziebandt AK, Engelmann S, Hecker M, de Jong A, Harmsen HJ, Raangs GC, Stokroos I, Arends JP, Dubois JY, van Dijl JM (2006) Mapping the pathways to staphylococcal pathogenesis by comparative secretomics. Microbiol Mol Biol Rev 70: 755–788

    Article  PubMed  CAS  Google Scholar 

  • Soto GE, Dodson KW, Ogg D, Liu C, Heuser J, Knight S, Kihlberg J, Jones CH, Hultgren SJ (1998) Periplasmic chaperone recognition motif of subunits mediates quaternary interactions in the pilus. Embo J 17: 6155–6167

    Article  PubMed  CAS  Google Scholar 

  • Stathopoulos C, Hendrixson DR, Thanassi DG, Hultgren SJ, St Geme JW 3rd, Curtiss R 3rd (2000) Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: an evolving story. Microbes Infect 2: 1061–1072

    Article  PubMed  CAS  Google Scholar 

  • Steiner D, Forrer P, Stumpp MT, Pluckthun A (2006) Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol 24: 823–831

    Article  PubMed  CAS  Google Scholar 

  • Szabady RL, Peterson JH, Skillman KM, Bernstein HD (2005) An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter. Proc Natl Acad Sci U S A 102: 221–226

    Article  PubMed  CAS  Google Scholar 

  • Tam PC, Maillard AP, Chan KK, Duong F (2005) Investigating the SecY plug movement at the SecYEG translocation channel. Embo J 24: 3380–3388

    Article  PubMed  CAS  Google Scholar 

  • Thanassi DG (2002) Ushers and secretins: channels for the secretion of folded proteins across the bacterial outer membrane. J Mol Microbiol Biotechnol 4: 11–20

    PubMed  CAS  Google Scholar 

  • Thanassi DG, Hultgren SJ (2000) Assembly of complex organelles: pilus biogenesis in gram-negative bacteria as a model system. Methods 20: 111–126

    Article  PubMed  CAS  Google Scholar 

  • Thanassi DG, Stathopoulos C, Karkal A, Li H (2005) Protein secretion in the absence of ATP: the autotransporter, two-partner secretion and chaperone/usher pathways of gram-negative bacteria (review). Mol Membr Biol 22: 63–72

    Article  PubMed  CAS  Google Scholar 

  • Thompson SA, Shedd OL, Ray KC, Beins MH, Jorgensen JP, Blaser MJ (1998) Campylobacter fetus surface layer proteins are transported by a type I secretion system. J Bacteriol 180: 6450–6458

    PubMed  CAS  Google Scholar 

  • Tjalsma H, Antelmann H, Jongbloed JD, Braun PG, Darmon E, Dorenbos R, Dubois JY, Westers H. Zanen G, Quax WJ, Kuipers OP, Bron S, Hecker M, van Dijl JM (2004) Proteomics of protein secretion by Bacillus subtilis: separating the “secrets” of the secretome. Microbiol Mol Biol Rev 68: 207–233

    Article  PubMed  CAS  Google Scholar 

  • Van den Berg B, Clemons WM Jr, Collinson I, Modis Y, Hartmann E, Harrison SC, Rapoport TA (2004) X-ray structure of a protein-conducting channel. Nature 427: 36–44

    Article  PubMed  CAS  Google Scholar 

  • van Ulsen P, van Alphen L, ten Hove J, Fransen F, van der Ley P, Tommassen J (2003) A Neisserial autotransporter NalP modulating the processing of other autotransporters. Mol Microbiol 50: 1017–1030

    Article  PubMed  CAS  Google Scholar 

  • Voulhoux R, Ball G, Ize B, Vasil ML, Lazdunski A, Wu LF, Filloux A (2001) Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. Embo J 20: 6735–6741

    Article  PubMed  CAS  Google Scholar 

  • Voulhoux R, Bos MP, Geurtsen J, Mols M, Tommassen J (2003) Role of a highly conserved bacterial protein in outer membrane protein assembly. Science 299: 262–265

    Article  PubMed  CAS  Google Scholar 

  • Vrontou E, Economou A (2004) Structure and function of SecA, the preprotein translocase nanomotor. Biochim Biophys Acta 1694: 67–80

    Article  PubMed  CAS  Google Scholar 

  • Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694: 299–310

    Article  PubMed  CAS  Google Scholar 

  • Yamane K, Bunai K, Kakeshita H (2004) Protein traffic for secretion and related machinery of Bacillus subtilis. Biosci Biotechnol Biochem 68: 2007–2023

    Article  PubMed  CAS  Google Scholar 

  • Zhu C, Ruiz-Perez F, Yang Z, Mao Y, Hackethal VL, Greco KM, Choy W, Davis K, Butterton JR, Boedeker EC (2006) Delivery of heterologous protein antigens via hemolysin or autotransporter systems by an attenuated ler mutant of rabbit enteropathogenic Escherichia coli. Vaccine 24: 3821–3831

    Article  PubMed  CAS  Google Scholar 

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Stathopoulos, C., Yen, Y.T., Tsang, C., Cameron, T. (2008). Protein Secretion in Bacterial Cells. In: El-Sharoud, W. (eds) Bacterial Physiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74921-9_5

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