Abstract
Bumblebees are important pollinators in both natural and agricultural settings. Bombus terrestris, the European buff-tailed bumblebee, is of particular economic importance in the pollination of many greenhouse crops. It is also a model organism in basic research in fields such as ecology, evolutionary biology, and physiology. Particularly, it is an emerging model species for quantitative and population genetics. Several genetic linkage maps have been produced for this species. Map construction is facilitated by the large numbers of haploid sons which may be produced by an individual queen of this primitively eusocial hymenopteran. These haploid males are genetically equivalent to gametes, and allow the direct estimation of the recombination frequency in the mother. A core linkage map of 14 homologous linkage groups has been determined. This species’ total recombination genome length has been consistently estimated at around 2,700–2,800 cM using a methods-of-moments approach. The genomic recombination rate is thus estimated to be 226 kb/cM. Other genomic tools developed for B. terrestris include a bacterial artificial chromosome (BAC) library as well as several cDNA libraries enriched with genes relevant for caste determination. Research in bumblebee genomics also profits greatly from developments in the honeybee Apis mellifera. For example, sequence homology between these hymenoptera is great enough to allow the isolation of honeybee candidate genes in the bumblebee. Genetic linkage maps have so far been used for mapping quantitative trait loci (QTL) for host-parasite susceptibility, immune defense, and male sexual investment. Additionally, the sex determination locus has been mapped. QTL mapping not only allows insights into the genetic architecture of fitness-relevant traits, but also may enable molecular assisted breeding for pathogen resistance or improved agricultural traits.
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References
Alaux C, Jaisson P, Hefetz A (2005) Reproductive decision-making in semelparous colonies of the bumblebee Bombus terrestris. Behav Ecol Sociobiol 59:270–277
Andersson L (2001) Genetic dissection of phenotypic diversity in farm animals. Nat Rev Genet 2:130–138
Baer B, Schmid-Hempel P (1999) Experimental variation in polyandry affects parasite loads and fitness in a bumble-bee. Nature 397:151–154
Beye M, Hasselmann M, Fondrk MK, Page RE, Omholt SW (2003) The gene csd is the primary signal for sexual development in the honeybee and encodes an sr-type protein. Cell 114:419–429
Cameron SA, Williams PH (2003) Phylogeny of bumble bees in the new world subgenus Fervidobombus (Hymenoptera: Apidae): congruence of molecular and morphological data. Mol Phylogenet Evol 28:552–563
Chakravarti A, Lasher LK, Reefer JE (1991) A maximum-likelihood method for estimating genome length using genetic-linkage data. Genetics 128:175–182
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196
Cook JM (1993) Sex determination in the hymenoptera: a review of models and evidence. Heredity 71:421–435
Dag A, Kammer Y (2001) Comparison between the effectiveness of honey bee (Apis mellifera) and bumble bee (Bombus terrestris) as pollinators of greenhouse sweet pepper (Capsicum annuum). Am Bee J 141:447–448
Duchateau MJ, Hoshiba H, Velthuis HHW (1994) Diploid males in the bumble-bee Bombus terrestris sex determination, sex alleles and viability. Entomol Exp Appl 71:263–269
Estoup A, Scholl A, Pouvreau A, Solignac M (1995) Monoandry and polyandry in bumble bees (Hymenoptera: Bombinae) as evidenced by highly variable microsatellites. Mol Ecol 4:89–93
Gadau J, Gerloff CU, Kruger N, Chan H, Schmid-Hempel P, Wille A, Page RE (2001) A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera: Apidae). Heredity 87:234–242
Gerloff CU, Ottmer BK, Schmid-Hempel P (2003) Effects of inbreeding on immune response and body size in a social insect, Bombus terrestris. Funct Ecol 17:582–589
Ghazoul J (2005) Buzziness as usual? Questioning the global pollination crisis. Trends Ecol Evol 20:367–373
Gorman MJ, Severson DW, Cornel AJ, Collins FH, Paskewitz SM (1997) Mapping a quantitative trait locus involved in melanotic encapsulation of foreign bodies in the malaria vector, Anopheles gambiae. Genetics 146:965–971
Goulson D (2003) Bumblebees – Their Behaviour and Ecology. Oxford University Press, New York
Hamilton WD, Axelrod R, Tanese R (1990) Sexual reproduction as an adaptation to resist parasites (a review). Proc Natl Acad Sci U S A 87:3566–3573
Hasselmann M, Fondrk MK, Page RE, Beye M (2001) Fine scale mapping in the sex locus region of the honey bee (Apis mellifera). Insect Mol Biol 10:605–608
Hulbert SH, Ilott TW, Legg EJ, Lincoln SE, Lander ES, Michelmore RW (1988) Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics 120:947–958
Hunt GJ, Page RE (1995) Linkage map of the honey-bee, Apis mellifera, based on RAPD markers. Genetics 139:1371–1382
Ings TC, Schikora J, Chittka L (2005) Bumblebees, humble pollinators or assiduous invaders? A population comparison of foraging performance in Bombus terrestris. Oecologia 144:508–516
Kaslow DC, Welburn SC (1996) Insect-transmitted pathogens in the insect midgut. In: Lehane MJ, Billingsley PF (eds) Biology of the Insect Midgut. Chapman and Hall, London, pp 432–462
Kawakita A, Sota T, Ito M, Ascher JS, Tanaka H, Kato M, Roubik DW (2004) Phylogeny, historical biogeography, and character evolution in bumble bees (Bombus: Apidae) based on simultaneous analysis of three nuclear gene sequences. Mol Phylogenet Evol 31:799–804
Little TJ (2002) The evolutionary significance of parasitism: do parasite-driven genetic dynamics occur ex silico? J Evol Biol 15:1–9
Lopez-Vaamonde C, Koning JW, Brown RM, Jordan WC, Bourke AFG (2004) Social parasitism by male-producing reproductive workers in a eusocial insect. Nature 430:557–560
Michener CD (2000) The Bees of the World. John Hopkins University Press, Baltimore
Morandin LA, Laverty TM, Kevan PG (2001) Bumble bee (Hymenoptera: Apidae) activity and pollination levels in commercial tomato greenhouses. J Econ Entomol 94:462–467
Moret Y, Schmid-Hempel P (2000) Survival for immunity: the price of immune system activation for bumblebee workers. Science 290:1166–1168
Pereboom JJM, Jordan WC, Sumner S, Hammond RL, Bourke AFG (2005) Differential gene expression in queen-worker caste determination in bumble-bees. Proc R Soc Lond B 272:1145–1152
Schmid-Hempel R, Schmid-Hempel P (2000) Female mating frequencies in Bombus spp. from central Europe. Insect Soc 47:36–41
Schmid-Hempel P, Puhr K, Kruger N, Reber C, Schmid-Hempel R (1999) Dynamic and genetic consequences of variation in horizontal transmission for a microparasitic infection. Evolution 53:426–434
Sirviö A, Gadau J, Rueppell O, Lamatsch D, Boomsma JJ, Pamilo P, Page RE (2006) High recombination frequency creates genotypic diversity in colonies of the leaf-cutting ant Acromyrmex echinatior. J Evol Biol 19:1475–1485
Siva-Jothy MT, Moret Y, Rolff J (2005) Insect immunity: an evolutionary ecology perspective. Adv Insect Physiol 32:1–48
Slate J (2005) Quantitative trait locus mapping in natural populations: progress, caveats and future directions. Mol Ecol 14:363–379
The Honeybee Genome Sequencing Consortium (2006) Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443:931–946
Velthuis HHW, van Doorn A (2006) A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie 37:421–451
Whiting PW (1943) Multiple alleles complementary sex determination of Habrobracon. Genetics 28:365–382
Wilfert L, Gadau J, Schmid-Hempel P (2006) A core linkage map of the bumblebee Bombus terrestris. Genome 49:1215–1226
Wilfert L, Gadau J, Baer B, Schmid-Hempel P (2007a) Natural variation in the genetic architecture of a host-parasite interaction in the bumblebee Bombus terrestris. Mol Ecol 16:1327–1339
Wilfert L, Gadau J, Schmid-Hempel P (2007b) The genetic architecture of immune defense and reproduction in male Bombus terestris bumblebees. Evolution 61:804–815
Zayed A (2004) Effective population size in hymenoptera with complementary sex determination. Heredity 93:627–630
Zheng LB, Cornel AJ, Wang R, Erfle H, Voss H, Ansorge W, Kafatos FC, Collins FH (1997) Quantitative trait loci for refractoriness of Anopheles gambiae to Plasmodium cynomolgi b. Science 276:425–428
Zheng LB, Wang S, Romans P, Zhao HY, Luna C, Benedict MQ (2003) Quantitative trait loci in Anopheles gambiae controlling the encapsulation response against Plasmodium cynomolgi ceylon. BMC Genetics 4:16
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Wilfert, L., Schmid-Hempel, P., Gadau, J. (2008). Bumblebee. In: Genome Mapping and Genomics in Arthropods. Genome Mapping Genomics Animals, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73833-6_2
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DOI: https://doi.org/10.1007/978-3-540-73833-6_2
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