Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Borneman J (1999) Culture-independent identification of microorganisms that respond to specified stimuli. Appl Environ Microbiol 65:3398–3400
Borneman J, Triplett EW (1997) Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation. Appl Environ Microbiol 63:2647–2653
Clark I, Atkins S (2004) Microbial environmental surveillance — a molecular biology manual. IACR, Rothamsted, pp 50
Fisher MM, Triplett EW (1999) Automated approach to ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities. Appl Environ Microbiol 65:4630–4636
Gelsomino A, Keijzer WAC, Cacco G, Van Elsas JD (1999) Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J Microbiol Methods 38:1–15
Heuer H, Krsek M, Baker P, Smalla K, Wellington EMH (1997) Analysis of actinomycetes communities by specific amplification of genes encoding 16S rRNA and gel electrophoretic separation in denaturing gradients. Appl Environ Microbiol 63:3233–3244
Kirk JL, Beaudette LA, Hart M, Moutoglis P, Klironomos JN, Lee H, Trevors JT (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58:169–188
Marschner P, Baumann K, Solaiman Z (2005) Molecular approaches to study the microbial community structure and function in the rhizosphere. In: Podila GK, Varma A (eds) Biotechnological applications of microbes. I.K. International, New Delhi, pp 311–334
Muyzer G, De Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes encoding for 16S rRNA. Appl Environ Microbiol 59:695–700
Ranjard L, Poly F, Lata J-C, Mougel C, Thioulous J, Nazaret S (2001) Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability. Appl Environ Microbiol 67:4479–4487
Schwieger F, Tebbe CC (1997) Efficient and accurate PCR amplification and detection of a recombinant gene in DNA directly extracted from soil using the expand™ high fidelity PCR system and T4 gene 32 protein. Biochemica 2:21–23
Vainio EJ, Hantula J (2000) Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA. Mycol Res 104:927–936
Van Elsas JD, Wolters A (1995) Polymerase chain reaction (PCR) analysis of soil microbial DNA. In: Akkermans ADL, Elsas Van JD, De Bruin (eds) Molecular and microbial ecology mannual. Kluwer, Dordrecht, pp 1–10
Wechter P, Williamson J, Robertson A, Kluepfel D (2003) A rapid, cost-effective procedure for the extraction of microbial DNA from soil. World J Microbiol Biotechnol 19:85–91
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Yang CH, Crowley DE (2000) Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. Appl Environ Microbiol 66:345–351
Yin B, Crowley DE, Sparovek G, De Melo WJ, Borneman J (2000) Bacterial functional redundancy along a soil reclamation gradient. Appl Environ Microbiol 66:4361–4365
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Solaiman, Z., Marschner, P. (2007). DGGE and RISA Protocols for Microbial Community Analysis in Soil. In: Varma, A., Oelmüller, R. (eds) Advanced Techniques in Soil Microbiology. Soil Biology, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70865-0_11
Download citation
DOI: https://doi.org/10.1007/978-3-540-70865-0_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-70864-3
Online ISBN: 978-3-540-70865-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)