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Sample Acquisition and Test Requisition

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Molecular Diagnostics in Cytopathology

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Abstract

In the modern era of cytopathology, sample acquisition refers to the process of procuring specimen material for both cytopathologic examination and ancillary testing. Depending on the site, sample acquisition can be categorized as either exfoliative or aspiration which refers to how material is obtained. Fine-needle aspiration (FNA) is a type of aspiration specimen procurement used to target a specific area not amenable to exfoliative techniques. Mastery of the FNA technique is critical in order to obtain optimal material for cytopathologic examination and further testing. Often, rapid on-site evaluation (ROSE) is utilized in aspiration cytopathology which allows for on-site and real-time specimen adequacy evaluation and allows for appropriate specimen triage during the procedure. After specimen collection, an appropriate test requisition should accompany the specimen to the cytopathology laboratory. This ensures the correct sample is matched with the correct patient and that the sample is appropriately handled.

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Abbreviations

CNB:

Core-needle biopsy

EBUS:

Endobronchial ultrasound

EUS:

Endoscopic ultrasound

FNA:

Fine-needle aspiration

ROSE:

Rapid on-site evaluation

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Author information

Authors and Affiliations

  1. Memorial Pathology Consultants, P.A, Kingwood, TX, USA

    Ross A. Miller

  2. Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX, USA

    Ashwyna Sunassee

Authors
  1. Ross A. Miller
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  2. Ashwyna Sunassee
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Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Sinchita Roy-Chowdhuri

  2. Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Paul A. VanderLaan

  3. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    John M. Stewart

  4. Department of Laboratory Medicine, University of Toronto, Toronto, ON, Canada

    Gilda da Cunha Santos

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Miller, R.A., Sunassee, A. (2019). Sample Acquisition and Test Requisition. In: Roy-Chowdhuri, S., VanderLaan, P., Stewart, J., Santos, G. (eds) Molecular Diagnostics in Cytopathology. Springer, Cham. https://doi.org/10.1007/978-3-319-97397-5_3

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  • DOI: https://doi.org/10.1007/978-3-319-97397-5_3

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-97396-8

  • Online ISBN: 978-3-319-97397-5

  • eBook Packages: MedicineMedicine (R0)

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