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Siberian Sturgeon Sperm Cryoconservation

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The Siberian Sturgeon (Acipenser baerii, Brandt, 1869) Volume 2 - Farming

Abstract

Sperm cryopreservation can potentially be used as a management tool for better organisation of spawning and improvements of breeding programs, providing a security strategy against decline in natural populations and incorporating of genes from wild fish into hatchery populations. Two extenders are presently available to insure high-quality cryopreserved Siberian sturgeon semen: (1) an extender consisting of 30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, 10% methanol and pH 8.0 and (2) a simplified extender consisting of just 0.1 M glucose and 15% methanol. Cryopreservation protocols secure good level of post-thaw motility (20–60% vs. 40–80% for fresh semen). A high variability in usefulness of semen for cryopreservation is a main problem in successful freezing. As such, it is difficult to predict a priori which semen samples would be successfully cryopreserved. Sperm characteristics, such as motility, have only limited usefulness, and more research towards identification of reliable markers of cryopreservation is needed. At present, implementation of cryopreserved semen into hatchery practices and breeding programs is limited despite the fact that this challenging task can greatly improve breeding programs for cultured Siberian sturgeon and help to protect diversity of wild populations. So far, the main application for cryopreservation of sturgeon semen has been for hybridization.

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Notes

  1. 1.

    Dimethyl sulfoxide.

  2. 2.

    Computer-assisted sperm analysis.

  3. 3.

    Average path velocity.

  4. 4.

    Curvilinear velocity.

  5. 5.

    Straight-line velocity.

  6. 6.

    Linearity.

  7. 7.

    Amplitude of lateral head displacement.

References

  • Alavi SMH, Hatef A, Pšenička M, Kašpar V, Boryshpolets S, Dzyuba B, Cosson J, Bondarenko V, Rodina M, Gela D, Linhart O (2012) Sperm biology and control of reproduction in sturgeon: (II) sperm morphology, acrosome reaction, motility and cryopreservation. Rev Fish Biol Fish 22:861–886

    Article  Google Scholar 

  • Billard R, Lecointre G (2001) Biology and conservation of sturgeon and paddlefish. Rev Fish Biol Fish 10:355–392

    Article  Google Scholar 

  • Billard R, Cosson J, Noveiri SB, Pourkazemi M (2004) Cryopreservation and short-term storage of sturgeon sperm, a review. Aquaculture 236:1–9

    Article  CAS  Google Scholar 

  • Brown GG, Mims SD (1999) Cryopreservation of paddlefish Polyodon spathula milt. J World Aquacult Soc 30:245–249

    Article  Google Scholar 

  • Ciereszko A, Toth GP, Christ SA, Dabrowski K (1996) Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa. Theriogenology 45:665–672

    Article  CAS  Google Scholar 

  • Cloud JG, Miller W, Levanduski MJ (1990) Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Prog Fish Cult 52:51–53

    Article  Google Scholar 

  • Dzyuba B, Boryshpolets S, Shaliutina A, Rodina M, Yamaner G, Gela D, Dzyuba V, Linhart O (2012) Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping. Aquaculture 356:272–278

    Article  Google Scholar 

  • Glogowski J, Kolman R, Szczepkowski M, Horváth A, Urbányi B, Sieczyński P, Rzemieniecki A, Domagała J, Demianowicz W, Kowalski R, Ciereszko A (2002) Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture 211:367–373

    Article  CAS  Google Scholar 

  • Horváth Á, Urbányi, B (2000) Cryopreservation of sterlet (Acipenser ruthenus) sperm. In: Norberg B, Kjesbu OS, Taranger GL, Andersson E, Stefansson SO (eds) Proceedings of 6th International Symposium on Reproductive Physiology of Fish, Bergen, Norway, 4–9 July, 1999, p 441

    Google Scholar 

  • Horváth Á, Wayman WR, Urbányi B, Ware KM, Dean JC, Tiersch TR (2005) The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North American sturgeon species. Aquaculture 247:243

    Article  Google Scholar 

  • Horvath A, Urbanyi B, Mims SD, Bean WB, Gomelsky B, Tiersch TR (2006) Improved cryopreservation of sperm of paddlefish (Polyodon spathula). J World Aquacult Soc 37:356–362

    Article  Google Scholar 

  • Horvath A, Urbanyi B, Mims SD (2009) Cryopreservation of sperm from species of the order Acipenseriformes. In: Cabrita E, Robles V, Herraez P (eds) Methods in reproductive aquaculture, marine and freshwater species. Section V. Boca Raton, CRC Press, Taylor & Francis Group, pp 409–415

    Google Scholar 

  • Horvath A, Urbanyi B, Wang C, Onders RJ, Mims SD (2010) Cryopreservation of paddlefish sperm in 5-mL straws. J Appl Ichthyol 26:715–719

    Article  Google Scholar 

  • Jähnichen H, Warnecke D, Trolsch E, Kohlmann K, Bergler H, Pluta HJ (1999) Motility and fertilizing capability of cryopreserved Acipenser ruthenus L. sperm. J Appl Ichthyol 15:204–206

    Article  Google Scholar 

  • Judycka S, Szczepkowski M, Ciereszko A, Sarosiek B, Słowińska M, Karol H, Liszewska E, Dryl K, Demianowicz W, Dietrich GJ (2015a) Characterization of Siberian sturgeon (Acipenser baerii Brandt) sperm obtained out of season. J Appl Ichthyol 31(S1):34–40

    Article  CAS  Google Scholar 

  • Judycka S, Szczepkowski M, Ciereszko A, Dietrich GJ (2015b) New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Cryobiology 70:184–189

    Article  CAS  Google Scholar 

  • Kopeika EE, Williot P, Goncharov BE (2000) Cryopreservation of Atlantic sturgeon Acipenser sturio L., 1758 sperm: first results and associated problems. Bol Inst Esp Oceanogr 16:167–173

    Google Scholar 

  • Leung LK-P (1991) Principles of biological cryopreservation. In: Jamieson BGM (ed) Fish evolution and systematics: evidence from spermatozoa. Cambridge University Press, Cambridge, pp 231–244

    Google Scholar 

  • Linhart O, Mims SD, Gomelsky B, Cvetkova LI, Cosson J, Rodina M, Horvath A, Urbanyi B (2006) Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa. J Appl Ichthyol 22:389–394

    Article  Google Scholar 

  • Martínez-Páramo S, Pérez-Cerezales S, Gómez-Romano F, Blanco G, Sanchez JA, Herraez MP (2009) Cryobanking as tool for conservation of biodiversity: effect of brown trout sperm cryopreservation on the male genetic potential. Theriogenology 71:594–604

    Article  Google Scholar 

  • Mazur P, Leibo SP, Seidel GE (2008) Cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions. Biol Reprod 78:2–12

    Article  CAS  Google Scholar 

  • Pšenička M, Dietrich GJ, Wojtczak M, Nynca J, Rodina M, Linhart O, Cosson J, Ciereszko A (2008) Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO. Cryobiology 56:251–253

    Article  Google Scholar 

  • Robles V, Cabrita E, Herraez MP (2009) Germplasm cryobanking in zebrafish and other aquarium model species. Zebrafish 6:281–293

    Article  Google Scholar 

  • Rochard E., Williot P., Castelnaud G. et Lepage M., 1991. Eléments de systématique et de biologie des populations sauvages d’esturgeons. In: Acipenser, P. Williot (ed) Cemagref Publication, Antony, pp 475-507

    Google Scholar 

  • Sarosiek B, Ciereszko A, Rzemieniecki A, Domagała J, Glogowski J (2004) The influence of semen cryopreservation on the release of some enzymes from Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) spermatozoa. Arch Pol Fish 12:13–21

    Google Scholar 

  • Shaliutina A, Dzyuba B, Hulak M, Boryshpolets S, Li P, Linhart O (2012) Evaluation of spermiation indices with multiple sperm collections in endangered sterlet (Acipenser ruthenus). Reprod Domest Anim 47:479–484

    Article  CAS  Google Scholar 

  • Shaliutina A, Hulak M, Li P, Sulc M, Dzyuba B, Linhart O (2013) Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus). Reprod Domest Anim 48:156–159

    Article  CAS  Google Scholar 

  • Sieczyński P, Cejko BI, Grygoruk C, Glogowski J (2015) Cryopreservation of Siberian sturgeon (Acipenser baerii, Brandt, 1869) and sterlet (Acipenser ruthenus, Linnaeus, 1758) semen and its influence on sperm motility parameters assessed using a computer-assisted sperm analysis (CASA) system. J Appl Ichthyol 31(S1):99–103

    Article  Google Scholar 

  • Suquet M, Dreanno C, Petton B, Normant Y, Omnes MH, Billard R (1998) Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa. Aquat Living Resour 11:45–48

    Article  Google Scholar 

  • Tiersch TR (2011) Introduction to the second edition. In: Tiersch TR, Green CC (eds) Cryopreservation in aquatic species, 2nd edn. World Aquaculture Society, Baton Rouge, pp 1–17

    Google Scholar 

  • Tsvetkova LI, Cosson J, Linhart O, Billard R (1996) Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser baeri and A. ruthenus. J Appl Ichthyol 12:107–112

    Article  Google Scholar 

  • Urbanyi B, Horvath A, Kovacs B (2004) Successful hybridization of Acipenser species using cryopreserved sperm. Aquac Int 12:47–56

    Article  Google Scholar 

  • Williot P, Kopeika EF, Goncharov BF (2000) Influence of testis state, temperature and delay in semen collection on spermatozoa motility in the cultured Siberian sturgeon (Acipenser baeri Brandt). Aquaculture 189:53–61

    Article  Google Scholar 

  • Williot P, Rouault T, Rochard E, Castelnaud G, Lepage M, Gonthier P, Elie P (2004) French attempts to protect and restore Acipenser sturio in the Gironde: status and perspectives, the research point of view. In: Gessner J, Ritterhoff J (eds) Species differentiation and population identification in the sturgeons Acipenser sturio L. and Acipenser oxyrinchus Mitchill, vol 101. Bundesmat für Naturschutz, Bonn, pp 83–89

    Google Scholar 

Download references

Acknowledgments

The study was financially supported by COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE), the Ministry of Education, Youth and Sports of the Czech Republic—projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024) and “CENAKVA II” (No. LO1205 under the NPU I program)—and by the Czech Science Foundation (No. P502/13/26952S) and the National Science Centre granted for research project (No. 2011/01/D/NZ9/03738) and funds appropriated to Institute of Animal Reproduction and Food Research of Polish Academy of Sciences. Authors also express thanks to prof. MSc. William L. Shelton, Ph.D. for English corrections.

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Authors and Affiliations

  1. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747, Olsztyn, Poland

    Andrzej Ciereszko

  2. Faculty of Fisheries and Protection of Waters, Laboratory of Germ Cells, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic

    Martin Pšenička

Authors
  1. Andrzej Ciereszko
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Correspondence to Andrzej Ciereszko .

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Editors and Affiliations

  1. Audenge, France

    Patrick Williot

  2. La Teste de Buch, France

    Guy Nonnotte

  3. State Center for Sturgeon Gene Pool Conservation “Kubanbioresursi”, Kuban State University, Krasnodar, Russia

    Mikhail Chebanov

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Ciereszko, A., Pšenička, M. (2018). Siberian Sturgeon Sperm Cryoconservation. In: Williot, P., Nonnotte, G., Chebanov, M. (eds) The Siberian Sturgeon (Acipenser baerii, Brandt, 1869) Volume 2 - Farming. Springer, Cham. https://doi.org/10.1007/978-3-319-61676-6_3

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