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Peripheral Blood Hematopoietic Progenitor Cell Graft Thawing

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Best Practices in Processing and Storage for Hematopoietic Cell Transplantation

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Abstract

Hematopoietic cell transplants (HCTs) have been performed for decades, and the number of both autologous and allogeneic transplants is constantly increasing. Mobilized peripheral blood (PB) hematopoietic progenitor cells (HPCs) are the most common cell source for adult patients, replacing almost completely the traditional bone marrow (BM) graft. Cryopreservation of PB HPC graft allows collecting and storing the product for later use. Cryopreservation is an essential practice for autologous products but can also be helpful for allogeneic one. The cryopreservation process itself can decrease cell viability and potency, and some of the reagents that are used to protect cells during cryopreservation can have toxic effects at thaw. Maintaining functional and viable HPCs is fundamental for HCT success. Thawing approaches of cryopreserved PB HPC should either minimize cell exposure time to toxic elements or include measures to rapidly remove them after thawing.

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Authors and Affiliations

  1. Department of Transfusion Medicine and Cellular Therapy, New York Presbyterian Hospital - Weill Cornell Medical Center, New York, NY, USA

    Ronit Reich-Slotky Ph.D., M.Sc.

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  1. Ronit Reich-Slotky Ph.D., M.Sc.
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Correspondence to Ronit Reich-Slotky Ph.D., M.Sc. .

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Editors and Affiliations

  1. Department of Pathology & Cell Biology, Columbia University, New York, New York, USA

    Joseph Schwartz

  2. New York Blood Center, New York, New York, USA

    Beth H. Shaz

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Reich-Slotky, R. (2018). Peripheral Blood Hematopoietic Progenitor Cell Graft Thawing. In: Schwartz, J., Shaz, B. (eds) Best Practices in Processing and Storage for Hematopoietic Cell Transplantation . Advances and Controversies in Hematopoietic Transplantation and Cell Therapy. Springer, Cham. https://doi.org/10.1007/978-3-319-58949-7_7

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  • DOI: https://doi.org/10.1007/978-3-319-58949-7_7

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  • Publisher Name: Springer, Cham

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  • Online ISBN: 978-3-319-58949-7

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