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Routine Hematopoietic Progenitor Cell Processing: HPC, Apheresis and HPC, Marrow Products

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Best Practices in Processing and Storage for Hematopoietic Cell Transplantation

Abstract

Upon collection of hematopoietic progenitor cells (HPCs), there are numerous steps that must take place to guarantee the potency and purity of the product in anticipation of transplantation. Every HPC product must have cell enumeration, flow cytometric immunophenotypic studies, sterility testing, viability studies, and, if necessary, cryopreservation and storage. It is critical that cryopreserved HPC products, which may be stored for years, be kept in a way that permits the cells to remain viable and functional in order for engraftment to occur. In this chapter, laboratory processes specifically pertaining to HPC, Apheresis and HPC, Marrow products will be discussed.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Jay S. Raval M.D. & Yara A. Park M.D.

  2. Advanced Cellular Therapeutics Facility, UNC Lineberger Comprehensive Cancer Center, Chapel Hill, NC, USA

    Kathryn McKay M.S., M.T. (A.S.C.P.)

Authors
  1. Jay S. Raval M.D.
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  2. Kathryn McKay M.S., M.T. (A.S.C.P.)
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  3. Yara A. Park M.D.
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Corresponding author

Correspondence to Jay S. Raval M.D. .

Editor information

Editors and Affiliations

  1. Department of Pathology & Cell Biology, Columbia University, New York, New York, USA

    Joseph Schwartz

  2. New York Blood Center, New York, New York, USA

    Beth H. Shaz

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Raval, J.S., McKay, K., Park, Y.A. (2018). Routine Hematopoietic Progenitor Cell Processing: HPC, Apheresis and HPC, Marrow Products. In: Schwartz, J., Shaz, B. (eds) Best Practices in Processing and Storage for Hematopoietic Cell Transplantation . Advances and Controversies in Hematopoietic Transplantation and Cell Therapy. Springer, Cham. https://doi.org/10.1007/978-3-319-58949-7_4

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  • DOI: https://doi.org/10.1007/978-3-319-58949-7_4

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-58948-0

  • Online ISBN: 978-3-319-58949-7

  • eBook Packages: MedicineMedicine (R0)

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