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Advances in Diagnosis of Kingella kingae Disease

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Advances in Understanding Kingella kingae

Part of the book series: SpringerBriefs in Immunology ((BRIEFSIMMUN))

Abstract

Although Kingella kingae was first identified more than 50 years ago, it was not until the early 1990s that the role of this organism as a human pathogen was fully appreciated. K. kingae grows on routine solid culture media, but joint exudates contain inhibitory factors that interfere with recovery of the bacterium from primary cultures. Inoculation of synovial fluid aspirates into blood culture vials of a variety of commercial systems results in enhanced detection of K. kingae. Polymerase chain reaction assays that target the 16S rRNA gene with broad-range primers or K. kingae-specific DNA sequences are associated with a two- to five-fold increase in detection rate compared to culture methods, reducing the fraction of bacteriologically negative cases of septic arthritis.

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  1. Clinical Microbiology Laboratory, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, 84101, Israel

    Pablo Yagupsky

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  1. Physician-in-Chief, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA

    Joseph W. St. Geme, III

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Yagupsky, P. (2016). Advances in Diagnosis of Kingella kingae Disease. In: St. Geme, III, J. (eds) Advances in Understanding Kingella kingae. SpringerBriefs in Immunology. Springer, Cham. https://doi.org/10.1007/978-3-319-43729-3_5

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