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Remote Ischemic Postconditioning (RIPC) After GMH in Rodents

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Brain Edema XVI

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 121))

Abstract

Germinal matrix hemorrhage (GMH) is the most common and devastating neurological injury of premature infants, and current treatment approaches are ineffective. Remote ischemic postconditioning (RIPC) is a method by which brief limb ischemic stimuli protect the injured brain. We hypothesized that RIPC can improve outcomes following GMH in rats. Neonatal rats (P7) were subjected to either stereotactic ganglionic eminence collagenase infusion or sham surgery. Groups were as follows: sham (n = 0), GMH non-RIPC (n = 10), GMH + 1 week RIPC (n = 10), GMH + 2 weeks RIPC (n = 10). Neurobehavior analysis at the fourth week consisted of Morris water maze (MWM) and rotarod (RR). This was followed by euthanasia for histopathology on day 28. Both 1- and 2-week RIPC showed significant improvement in FF and RR motor testing compared with untreated animals (i.e., GMH without RIPC). RIPC treatment also improved cognition (MWM) and attenuated neuropathological ventricular enlargement (hydrocephalus) in juvenile animals following GMH. RIPC is a safe and noninvasive approach that improved sensorimotor and neuropathological outcomes following GMH in rats. Further studies are needed to evaluate for mechanisms of neuroprotection.

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Acknowledgment

This study was partially supported by the National Institutes of Health grant RO1 NS078755 (Dr Zhang).

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Authors and Affiliations

  1. Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA

    Tim Lekic, Damon Klebe, Jerry Flores, Regina Peters, William B. Rolland & Jiping Tang

  2. Department of Neurosurgery, School of Medicine, Loma Linda, CA, USA

    John H. Zhang MD, PhD

  3. Division of Physiology and Pharmacology, Loma Linda University School of Medicine, 11041 Campus Street, Risley Hall Rm 219, Loma Linda, CA, 92354, USA

    John H. Zhang MD, PhD

Authors
  1. Tim Lekic
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  2. Damon Klebe
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  3. Jerry Flores
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  4. Regina Peters
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  5. William B. Rolland
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  6. Jiping Tang
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  7. John H. Zhang MD, PhD
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Corresponding author

Correspondence to John H. Zhang MD, PhD .

Editor information

Editors and Affiliations

  1. Department of Anesthesiology, Loma Linda University School of Medicine, Loma Linda, California, USA

    Richard L. Applegate

  2. Department of Neurosurgery, Soochow University The First Affiliated Hospital, Suzhou, Jiangsu, China

    Gang Chen

  3. Department of Neurosurgery, Third Military Medical University Southwest Hospital, Chongqing, China

    Hua Feng

  4. Dept.of Anesthesiology, Physiology and Neurosurgery, Loma Linda University School of Medicine, Loma Linda, California, USA

    John H. Zhang

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Lekic, T. et al. (2016). Remote Ischemic Postconditioning (RIPC) After GMH in Rodents. In: Applegate, R., Chen, G., Feng, H., Zhang, J. (eds) Brain Edema XVI. Acta Neurochirurgica Supplement, vol 121. Springer, Cham. https://doi.org/10.1007/978-3-319-18497-5_11

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  • DOI: https://doi.org/10.1007/978-3-319-18497-5_11

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-18496-8

  • Online ISBN: 978-3-319-18497-5

  • eBook Packages: MedicineMedicine (R0)

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