Abstract
Immunohistochemistry (IHC), clonality analysis, and other molecular techniques are indispensable tools in the diagnosis of cutaneous lymphomas. Without the ability to identify cell surface and intracellular markers and to assess if a population is clonal, it would be impossible to accurately diagnose most lymphomas. This chapter offers a brief introduction to the use of immunohistochemistry and other molecular techniques in diagnosing cutaneous lymphomas.
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References
Coons AH, Creech HJ, Jones RN. Immunological properties of an antibody containing a fluorescent group. Exp Biol Med. 1941;47:200–2.
Mason TE, Phiefer RF, Spicer SS, Swallow RA, Dreskin RB. An immunoglobulin-enzyme bridge method for localizing tissue antigens. J Histochem Cytochem. 1969;17:563–9.
Nakane PK. Simultaneous localization of multiple tissue antigens using the peroxidase-labelled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem. 1968;16:557–60.
Leong AS. Diagnostic immunohistochemistry–problems and solutions. Pathology. 1992;24:1–4.
Taylor CR. An exaltation of experts: concerted efforts in the standardization of immunohistochemistry. Hum Pathol. 1994;25:2–11.
de Matos LL, Trufelli DC, de Matos MGL, da Silva Pinhal MA. Immunohistochemistry as an important tool in biomarker detection and clinical practice. Biomark Insights. 2010;5:9–20.
Murphy KM, Travers P, Walport M. Janeway’s immunobiology. 7th ed. New York: Garland Science; 2007.
Pigozzi B. Expression of the CD1a molecule in B-and T-lymphoproliferative skin conditions. Oncol Rep. 2006;15:347–51.
Martin LH, Calabi F, Milstein C. Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens. Proc Natl Acad Sci U S A. 1986;83:9154–8.
Schiefner A, Wilson IA. Presentation of lipid antigens by CD1 glycoproteins. Curr Pharm Des. 2009;15:3311–7.
Yang J, Ye Y, Carroll A, Yang W, Lee H. Structural biology of the cell adhesion protein CD2: alternatively folded states and structure-function relation. Curr Protein Pept Sci. 2001;2:1–17.
Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology. 7th ed. Philadelphia: Saunders Elsevier; 2007.
Gocke CD. Immunology of non-Hodgkin lymphoma. In: Dabbs D, editor. Diagnostic immunohistochemistry. 2nd ed. Philadelphia: Churchill Livingstone Elsevier; 2006. p. 137–61.
Aandahl EM, Sandberg JK, Beckerman KP, Taskén K, Moretto WJ, Nixon DF. CD7 is a differentiation marker that identifies multiple CD8 T cell effector subsets. J Immunol. 2003;170:2349–55.
Gao GF, Jakobsen BK. Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunol Today. 2000;21:630–6.
Nakayama F, Nishihara S, Iwasaki H, Kudo T, Okubo R, Kaneko M, et al. CD15 expression in mature granulocytes is determined by alpha 1,3-fucosyltransferase IX but in promyelocytes and monocytes by alpha 1,3-fucosyltransferase IV. J Biol Chem. 2001;276:16100–6.
Benner MF, Jansen PM, Meijer CJLM, Willemze R. Diagnostic and prognostic evaluation of phenotypic markers TRAF1, MUM1, BCL2 and CD15 in cutaneous CD30-positive lymphoproliferative disorders. Br J Dermatol. 2009;161:121–7.
Eberle FC, Song JY, Xi L, Raffeld M, Harris NL, Wilson WH, et al. Nodal involvement by cutaneous CD30-positive T-cell lymphoma mimicking classical Hodgkin lymphoma. Am J Surg Pathol. 2012;36:716–25.
Crocker J, Skilbeck N, Brown G, Bunce C. Presence of 3-fucosyl-N-acetyllactosamine shown by monoclonal antibody AGF 4.48 in Reed-Sternberg cells. J Clin Pathol. 1986;39:564–8.
Boonacker EP, Wierenga EA, Smits HH, Van Noorden CJF. CD26/DPPIV signal transduction function, but not proteolytic activity, is directly related to its expression level on human Th1 and Th2 cell lines as detected with living cell cytochemistry. J Histochem Cytochem. 2002;50:1169–77.
Chen X. Biochemical properties of recombinant prolyl dipeptidases DPP-IV and DPP8. Adv Exp Med Biol. 2006;575:27–32.
Yamamoto H, Kishimoto T, Minamoto S. NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27. J Immunol. 1998;161:4753–9.
Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, et al. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc Natl Acad Sci U S A. 1997;94:6346–51.
Tarkowski M. Expression and a role of CD30 in regulation of T-cell activity. Curr Opin Hematol. 2003;10:267–71.
Schneider C, Hübinger G. Pleiotropic signal transduction mediated by human CD30: a member of the tumor necrosis factor receptor (TNFR) family. Leuk Lymphoma. 2002;43:1355–66.
Mitsumoto Y, Oka S, Sakuma H, Inazawa J, Kawasaki T. Cloning and chromosomal mapping of human glucuronyltransferase involved in biosynthesis of the HNK-1 carbohydrate epitope. Genomics. 2000;65:166–73.
Holness CL, Simmons DL. Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood. 1993;81:1607–13.
Chiarle R, Voena C, Ambrogio C, Piva R, Inghirami G. The anaplastic lymphoma kinase in the pathogenesis of cancer. Nat Rev Cancer. 2008;8:11–23.
Takada K, Nanbo A. The role of EBERs in oncogenesis. Semin Cancer Biol. 2001;11:461–7.
Lord SJ, Rajotte RV, Korbutt GS, Bleackley RC. Granzyme B: a natural born killer. Immunol Rev. 2003;193:31–8.
Brenner I, Roth S, Puppe B, Wobser M, Rosenwald A, Geissinger E. Primary cutaneous marginal zone lymphomas with plasmacytic differentiation show frequent IgG4 expression. Mod Pathol. 2013;26:1568–76.
Riella LV, Paterson AM, Sharpe AH, Chandraker A. Role of the PD-1 pathway in the immune response. Am J Transplant. 2012;12:2575–87.
Thiery J, Keefe D, Boulant S, Boucrot E, Walch M, Martinvalet D, et al. Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells. Nat Immunol. 2011;12:770–7.
Weng J, Rawal S, Chu F, Park HJ, Sharma R, Delgado DA, et al. TCL1: a shared tumor-associated antigen for immunotherapy against B-cell lymphomas. Blood. 2012;120:1613–23.
Fu TB, Virgilio L, Narducci MG, Facchiano A, Russo G, Croce CM. Characterization and localization of the TCL-1 oncogene product. Cancer Res. 1994;54:6297–301.
Laine J, Künstle G, Obata T, Sha M, Noguchi M. The protooncogene TCL1 is an Akt kinase coactivator. Mol Cell. 2000;6:395–407.
Noguchi M, Ropars V, Roumestand C, Suizu F. Proto-oncogene TCL1: more than just a coactivator for Akt. FASEB J. 2007;21:2273–84.
Förch P, Puig O, Kedersha N, MartÃnez C, Granneman S, Séraphin B, et al. The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell. 2000;6:1089–98.
Förch P, Valcárcel J. Molecular mechanisms of gene expression regulation by the apoptosis-promoting protein TIA-1. Apoptosis. 2001;6:463–8.
Van Dongen JJM, Langerak AW, Brüggemann M, Evans PAS, Hummel M, Lavender FL, et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2003;17:2257–317.
Wood GS, Tung RM, Heaffner AC, Crooks CF, Liao S, Orozco R, et al. Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sezary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). J Invest Dermatol. 1994;103:34–41.
Wood GS. T-cell receptor and immunoglobulin gene rearrangements in diagnosing skin disease. Arch Dermatol. 2001;137:1503–6.
Dippel E, Assaf C, Hummel M, Schrag HJ, Stein H, Goerdt S, et al. Clonal T-cell receptor gamma-chain gene rearrangement by PCR-based GeneScan analysis in advanced cutaneous T-cell lymphoma: a critical evaluation. J Pathol. 1999;188:146–54.
Theodorou I, Raphaël M, Bigorgne C, Fourcade C, Lahet C, Cochet G, et al. Recombination pattern of the TCR gamma locus in human peripheral T-cell lymphomas. J Pathol. 1994;174:233–42.
Zhu D, Kadin ME, Samoszuk M. Detection of clonal T-cell receptor-gamma gene rearrangement by PCR/temporal temperature gradient gel electrophoresis. Am J Clin Pathol. 2001;116:527–34.
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Goyal, A., Goyal, K., Nardi, V., Hoang, M.P., Carter, J.B., Duncan, L.M. (2015). Immunohistochemistry and Molecular Techniques. In: Carter, J., Goyal, A., McDivitt Duncan, L. (eds) Atlas of Cutaneous Lymphomas. Springer, Cham. https://doi.org/10.1007/978-3-319-17217-0_3
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DOI: https://doi.org/10.1007/978-3-319-17217-0_3
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