Abstract
For an isotherm such as the Langmuir isotherm, it becomes linear if the concentration range is sufficiently low. Each component’s isotherm will be independent of the other components. There will be no competition among the components for binding sites. Analytical LC usually involves small or dilute samples. Components in the sample are quickly diluted and/or separated away from each other during migration inside a column. Thus, interference effects among different sample components are often negligible. With the rapid growth of biotechnology, preparative- and large-scale LC operations become more and more important. High-feed concentrations and large sample volumes are often used to increase productivity. In such cases, interference effects may no longer be ignored.
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Gu, T. (2015). Interference Effects in Multicomponent Chromatography. In: Mathematical Modeling and Scale-Up of Liquid Chromatography. Springer, Cham. https://doi.org/10.1007/978-3-319-16145-7_6
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DOI: https://doi.org/10.1007/978-3-319-16145-7_6
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