Abstract
Affinity chromatography is a powerful tool for the purification of enzymes, antibodies, antigens, and many other proteins and macromolecules that are important in scientific research and development of novel biological drugs. Affinity chromatography not only purifies a product, but also concentrates the product to a great extent [1]. Over the years, this subject has been reviewed by many people, including Chase [1] and Liapis [2]. Affinity chromatography is also called biospecific adsorption, since it utilizes the biospecific binding between solute molecules and immobilized ligands that is often compared with the fitting of a lock and its key. The monovalent binding between a ligand and a solute macromolecule is generally treated as second-order kinetics.
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References
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10.1 Electronic Supplementary Material
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Table 10.1
ParameterEstimationAffinity (XLS 31 KB)
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Gu, T. (2015). Modeling of Slow Kinetics and Affinity Chromatography. In: Mathematical Modeling and Scale-Up of Liquid Chromatography. Springer, Cham. https://doi.org/10.1007/978-3-319-16145-7_10
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DOI: https://doi.org/10.1007/978-3-319-16145-7_10
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