Skip to main content

Part of the book series: Proceedings in Adaptation, Learning and Optimization ((PALO,volume 2))

  • 1672 Accesses

Abstract

In corneal endothelium tissue engineering, automatic judgment is vital to determine whether cultured cells are suitable for transplantation, which can be achieved by measuring indicators from cell images. Indicator measurement requires an accurate image processing method for cell segmentation. We previously propose the system that combines simple image-processing filters suitably by genetic programming. However, it is too difficult to obtain high accuracy because of over-fitting. Therefore, achieving segmentation by an unsupervised learning method is an essential requirement for applying segmentation to several types of images. In this paper, we propose an unsupervised learning segmentation method using binarization and growing neural gas. This method segments the cells by performing vector quantization of cell borders and connecting units. The proposed method is comparable to a previously reported method. The results show that the proposed method has superior accuracy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Inaba, M.: Contact lens wear and corneal endothelial cell loss. Journal of the Eye 26(2), 187–192 (2009)

    MathSciNet  Google Scholar 

  2. Tsubota, K., Hato, S.: Corneal disease and regenerative medicine. Trends in the Sciences 15(7), 7–13 (2010)

    Article  Google Scholar 

  3. Koizumi, N., Nishida, K., Amano, S., Kinoshita, S.: Progress in the development of tissue engineering of the cornea in japan. Journal of Japanese Ophthalmological Society 111(7), 493–503 (2007)

    Google Scholar 

  4. Koizumi, N.: Cultivated corneal endothelial cell sheet transplantation in a primate model. Journal of Japanese Ophthalmological Society 113(11), 1050–1059 (2009)

    Google Scholar 

  5. Abrámoff, M.D., Magalhães, P.J., Ram, S.J.: Image processing with imagej. Biophotonics International 11(7), 36–42 (2004)

    Google Scholar 

  6. Carpenter, A.E., Jones, T.R., Lamprecht, M.R., Clarke, C., Kang, I.H., Friman, O., Guertin, D.A., Joo, C.H., Robert, L.A., Moffat, J., Golland, P., Sabatini, D.M.: Cellprofiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biology 7(10), R100.1–R100.11 (2006)

    Article  Google Scholar 

  7. Koza, J.R.: Genetic Programming on the Programming of Computers by Means of Natural Selection. MIT Press (1992)

    Google Scholar 

  8. Aoki, S., Nagao, T.: Automatic construction of tree-structural image transformations using genetic programming. In: Proceedings of the International Conference on Image Analysis and Processing, pp. 136–141 (1999)

    Google Scholar 

  9. Nakano, Y., Nagao, T.: 3d medical image processing using 3d-actit: Automatic construction of tree-structural image transformation (computer vision, medical applications and networked mm) (international workshop on advanced image technology (iwait2004)). IEICE technical report. Image Engineering 103(540), 49–53 (2004)

    Google Scholar 

  10. Hiroyasu, T., Fujita, S., Watanabe, A., Miki, M., Ogura, M., Fukumoto, M.: Comparison of gp and sap in the image-processing filter construction using pathology images. In: 2010 3rd International Congress on Image and Signal Processing (CISP), vol. 2, pp. 904–908 (2010)

    Google Scholar 

  11. Hiroyasu, T., Yamaguchi, H., Fujita, S., Miki, M., Yoshimi, M., Ogura, M., Fukumoto, M.: An algorithm for cancer nest feature extraction from pathological images. In: 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC, pp. 3423–3426 (2011)

    Google Scholar 

  12. Yamaguchi, H., Hiroyasu, T., Nunokawa, S., Koizumi, N., Okumura, N., Yokouchi, H., Miki, M., Yoshimi, M.: Comparison study of controlling bloat model of gp in constructing filter for cell image segmentation problems. In: 2012 IEEE Congress on Evolutionary Computation (CEC), pp. 1–8 (2012)

    Google Scholar 

  13. Hiroyasu, T., Nunokawa, S., Yamaguchi, H., Koizumi, N., Okumura, N., Yokouchi, H.: Algorithms for automatic extraction of feature values of corneal endothelial cells using genetic programming. In: 2012 Joint 6th International Conference on Soft Computing and Intelligent Systems (SCIS) and 13th International Symposium on Advanced Intelligent Systems (ISIS), pp. 1388–1392 (2012)

    Google Scholar 

  14. Bernd, F.: A growing neural gas network learns topologies. In: Advances in Neural Information Processing Systems 7, pp. 625–632 (1995)

    Google Scholar 

  15. Martin, D., Fowlkes, C., Tal, D., Malik, J.: A database of human segmented natural images and its application to evaluating segmentation algorithms and measuring ecological statistics. In: IEEE International Conference on Computer Vision, vol. 2, pp. 416–423 (2001)

    Google Scholar 

  16. Martinetz, T.M., Berkovich, S.G., Schulten, K.J.: ‘neural-gas’ network for vector quantization and its application to time-series prediction. IEEE Transactions on Neural Networks 4(4), 558–569 (1993)

    Article  Google Scholar 

  17. Sunakawa, K., Saito, T.: A-2-29 growing neural gas and skeletonization. In: Proceedings of the Society Conference of IEICE, vol. 60 (2010)

    Google Scholar 

  18. Hartigan, J.A., Wong, M.A.: Algorithm as 136: A k-means clustering algorithm. Journal of the Royal Statistical Society. Series C 28(1), 100–108 (1979)

    MATH  Google Scholar 

  19. Tamura, H.: A comparison of line thinning algorithms from digital geometry viewpoint. In: Proc. 4th Int. Conf. Pattern Recognition, 715–719 (1978)

    Google Scholar 

  20. Vincent, L., Soille, P.: Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Transactions on Pattern Analysis and Machine Intelligence 13(6), 583–598 (1991)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tomoyuki Hiroyasu .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this paper

Cite this paper

Hiroyasu, T., Sekiya, S., Koizumi, N., Okumura, N., Yamamoto, U. (2015). Cell Segmentation Using Binarization and Growing Neural Gas. In: Handa, H., Ishibuchi, H., Ong, YS., Tan, KC. (eds) Proceedings of the 18th Asia Pacific Symposium on Intelligent and Evolutionary Systems - Volume 2. Proceedings in Adaptation, Learning and Optimization, vol 2. Springer, Cham. https://doi.org/10.1007/978-3-319-13356-0_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-13356-0_15

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-13355-3

  • Online ISBN: 978-3-319-13356-0

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics