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TALEN-Based Genome Editing in Yeast

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Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

The development of nuclease-mediated Genome Editing represents a significant technological advance and may prove to be as important as PCR and DNA sequencing. Regardless, this technology puts the power of genome re-orchestration into the hands of research scientists for the first time and may lead to a paradigm shift in many biological fields. We present background information, so the reader has a greater understanding for the basis of this technology. We also discuss TAL effector nuclease (TALEN) as the latest engineered nuclease tool, its implementation and uses and present a yeast-based protocol that may be helpful in transitioning this technology to other fungal species.

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Acknowledgments

The work on TALEN technology development and application in the Yang lab and the Spalding lab have been funded by several funding agencies. The authors wish to acknowledge the US National Science Foundation (Award number 1238189 to B.Y. and MCB-0952323 to M.H.S.) and the US Department of Energy’s Advanced Research Projects Agency-Energy Program (DEAR0000010 to M.H.S.) and Office of Science, Basic Energy Science Division. (DE-FG02-12ER16335 to M.H.S.)

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Authors and Affiliations

  1. Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011, USA

    Ting Li, David A. Wright, Martin H. Spalding & Bing Yang

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  1. Ting Li
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  2. David A. Wright
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  3. Martin H. Spalding
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  4. Bing Yang
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Correspondence to Bing Yang .

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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Li, T., Wright, D.A., Spalding, M.H., Yang, B. (2015). TALEN-Based Genome Editing in Yeast. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_27

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