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Escherichia coli’s OmpA as Biosurfactant for Cosmetic Industry: Stability Analysis and Experimental Validation Based on Molecular Simulations

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Advances in Computational Biology

Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 232))

Abstract

The development of biosurfactants has increased due to their biodegradability, low toxicity and specificity. The aim of this work is to analyze the behavior of the outer membrane protein A (OmpA) of Escherichia coli by Molecular Dynamics simulations and to perform experimental validation when used as stabilizer for dodecane/water emulsions. Trajectories were analyzed with the analysis tools provided by the GROMACS package. OmpA was purified from E. coli K-12 W3110/pCA24N strain in medium with isopropylthio- β-galactoside. Oil in water emulsions with different concentrations of OmpA were prepared in batch processes. MD trajectories with OmpA reached stability after 1 ns with an average RMSD value of 5.6 nm and they showed that OmpA remains stable in emulsion. An inverse effect related to protein concentration was found on the stability of the emulsion. OmpA displayed a significant role as a stabilizer for dodecane/water emulsions as the presence of OmpA increased their stability up to 7.5 h.

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Authors and Affiliations

  1. Grupo de Diseño de Productos y Procesos (GDPP), Departamento de Ingeniería Química, Universidad de los Andes, Bogotá, Colombia

    Sonia Milena Aguilera Segura, Angie Paola Macías, Diana Carrero Pinto, Watson Lawrence Vargas, Oscar Alberto Álvarez & Andrés Fernando González Barrios

  2. Centro de Investigaciones Microbiológicas (CIMIC), Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotó, Colombia

    Martha Josefina Vives-Florez

  3. Grupo de Comunicaciones y Tecnología de la Información, Departamento de Ingeniería de Sistemas, Universidad de los Andes, Bogotó, Colombia

    Harold Enrique Castro Barrera

Authors
  1. Sonia Milena Aguilera Segura
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  2. Angie Paola Macías
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  3. Diana Carrero Pinto
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  4. Watson Lawrence Vargas
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  5. Martha Josefina Vives-Florez
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  6. Harold Enrique Castro Barrera
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  7. Oscar Alberto Álvarez
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  8. Andrés Fernando González Barrios
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Corresponding author

Correspondence to Sonia Milena Aguilera Segura .

Editor information

Editors and Affiliations

  1. University of Caldas, Manizales, Colombia

    Luis F. Castillo

  2. Cenicafé - Centro Nacional de Investigaciones del Café en Colombia, Chinchiná, Colombia

    Marco Cristancho

  3. University of Caldas, Manizales, Colombia

    Gustavo Isaza

  4. BIOS - Centro Bioinformática y Biologia Computacional de Colombia, Manizales, Colombia

    Andrés Pinzón

  5. Department of Computer Science School of Science, University of Salamanca, Salamanca, Spain

    Juan Manuel Corchado Rodríguez

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© 2014 Springer International Publishing Switzerland

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Segura, S.M.A. et al. (2014). Escherichia coli’s OmpA as Biosurfactant for Cosmetic Industry: Stability Analysis and Experimental Validation Based on Molecular Simulations. In: Castillo, L., Cristancho, M., Isaza, G., Pinzón, A., Rodríguez, J. (eds) Advances in Computational Biology. Advances in Intelligent Systems and Computing, vol 232. Springer, Cham. https://doi.org/10.1007/978-3-319-01568-2_38

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  • DOI: https://doi.org/10.1007/978-3-319-01568-2_38

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-01567-5

  • Online ISBN: 978-3-319-01568-2

  • eBook Packages: EngineeringEngineering (R0)

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