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Biological Tissue Imaging at Different Levels: MALDI and SIMS Imaging Combined

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Surface Analysis and Techniques in Biology

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Abstract

Mass spectrometry has been employed to analyze the composition and structure of biologically relevant molecules in solution. Advances in methodology and instrumentation developments now allow the application of mass spectrometry for local biomolecular analysis directly on biological tissue surfaces; this technique is called imaging mass spectrometry (IMS). IMS is an innovative discovery tool for the biomedical sciences. This chapter describes the two main approaches relevant for molecular tissue imaging studies in the life sciences: secondary ion mass spectrometry (SIMS) imaging and matrix-assisted laser desorption and ionization (MALDI)–based imaging techniques. The benefits of imaging mass spectrometry for the fields of drug metabolism, lipidomics, and proteomics are discussed. Integrated MS imaging and proteomics protocols as well as tandem-MS imaging strategies, which are key to the identification of larger-molecular-weight compounds, are also reviewed.

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  1. FOM-Institute for Atomic and Molecular Physics, Science park 104, 1098 XG, Amsterdam, The Netherlands

    J. Stauber & Ron M. A. Heeren

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  1. J. Stauber
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  1. Nanostructures and Surfaces Laboratory GE Global Research, Niskayuna, New York, USA

    Vincent S. Smentkowski

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Stauber, J., Heeren, R.M.A. (2014). Biological Tissue Imaging at Different Levels: MALDI and SIMS Imaging Combined. In: Smentkowski, V. (eds) Surface Analysis and Techniques in Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-01360-2_5

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