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Biomolecular Analysis by Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS)

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Surface Analysis and Techniques in Biology

Abstract

The use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) for biomolecular analysis has made tremendous progress in recent years. This chapter will outline the principal aspects of ToF-SIMS as well as recent technical developments that have made surface mass spectrometry so valuable for this field. Furthermore, an overview on relevant biochemical applications based on the four essential operational modes—spectrometry, imaging, depth profiling, and 3D analysis—will be given. The applications range from the analysis of Langmuir–Blodgett films and tissue sections to the analysis of whole cells. With these results in mind, we will discuss the chances and limitations of the technique.

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Notes

  1. 1.

    Lists of the composition and mass-to-charge ratio of typical secondary ions characteristic of amino acids can be found, for example, in a study by Michel et al. [55].

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Authors and Affiliations

  1. Tascon GmbH, Heisenbergstr. 15, 48155, Münster, Germany

    Daniel Breitenstein & Birgit Hagenhoff

  2. Tascon USA, Inc., 100 Red Schoolhouse Road, Chestnut Ridge, NY, 10977, USA

    Albert Schnieders (Managing Director)

  3. CNM Technologies, Bielefeld, Germany

    Albert Schnieders (Managing Director)

Authors
  1. Daniel Breitenstein
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  2. Birgit Hagenhoff
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  3. Albert Schnieders
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Correspondence to Daniel Breitenstein .

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Editors and Affiliations

  1. Nanostructures and Surfaces Laboratory GE Global Research, Niskayuna, New York, USA

    Vincent S. Smentkowski

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Breitenstein, D., Hagenhoff, B., Schnieders, A. (2014). Biomolecular Analysis by Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). In: Smentkowski, V. (eds) Surface Analysis and Techniques in Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-01360-2_3

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