Abstract
In this chapter, an optoelectronic Lab-on-Chip system for DNA amplification integrating a polydimethylsiloxane microfluidic structure with indium tin oxide heaters on a 5 × 5 cm2 microscope glass slide is presented. The microfluidic structure comprises a channel for the fluid handling and a reaction chamber for the polymerase chain reaction (PCR). Two lateral heaters, located at the inlet and outlet positions of the chamber, actuate the valves and allow the chamber isolation, while the last one, positioned below the chamber, is dedicated to the PCR thermal cycle. In order to optimize heaters and valves geometry, the temperature distribution over the heaters and the membrane valve deformation have been studied using the commercial software COMSOL Multiphysics. The microfluidic structure has been fabricated by using soft lithography techniques on one side of the glass substrate, while the thin film heaters were deposited and patterned on the opposite glass side. Experiments show that valve activation begins around 60 °C, and complete closure is observed around to 100 °C, without any loss of liquid from the chamber.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
D. Erickson, L. Dongqing. Integrated microfluidic devices. Analytica Chimica Acta, 507 (1), 11–26 (2004)
A.G. Crevillén, M. Hervás, M.A. López, M.C. González, A. Escarpa. Real sample analysis on microfluidic devices. Talanta, 74 (3), 342–357 (2007)
D. Janasek, J. Franzke, A. Manz. Scaling and the design of miniaturized chemical-analysis systems. Nature 442 (7101) 374–380 (2006)
D. Caputo, M. Ceccarelli, G. de Cesare, A. Nascetti, R. Scipinotti. Lab-on-glass system for DNA analysis using thin and thick film technologies. in Materials Research Society Symposia Proceedings, 1191, 53–58 (2009)
M.A. Northrup, R.F. Hills, P. Landre, S. Lehew, D. Hadley, R. Watson. A MEMS-based DNA analysis system. in Tranducer ’95, Eighth International Conference on Solid State Sens Actuators, Stockholm, Sweden. ISBN:9 1-630-3473-5, 764–767 (1995)
N.C. Cady, S. Stelick, M.V. Kunnavakkam, C.A. Batt. Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform. Sens. Actuators B Chem. 107, 332 – 341 (2005)
Z.Q Niu, W.Y. Chen, S.Y. Shao, X.Y. Jia, W.P. Zhang. DNA amplification on a PDMS–glass hybrid microchip. J. Micromech. and Microeng., 16, (2), 425–433 (2006).
D. Caputo, G. de Cesare, A. Nascetti, and R. Scipinotti. a-Si:H temperature sensor integrated in a thin film heater. Phys. Status Solidi A, 207 (3), 708–711 (2010).
M.A. Eddings, M.A. Johnson, B.K. Gale. Determining the optimal PDMS-PDMS bonding technique for microfluidic devices. J Micromech Microengineering, 18, (6), 06700 (2008).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer International Publishing Switzerland
About this paper
Cite this paper
Caputo, D. et al. (2014). Thermally Actuated Microfluidic System for Polymerase Chain Reaction Applications. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_5
Download citation
DOI: https://doi.org/10.1007/978-3-319-00684-0_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-00683-3
Online ISBN: 978-3-319-00684-0
eBook Packages: EngineeringEngineering (R0)