Abstract
DNA can be detected with the high precision and specificity of immunoelectron microscopy. For this purpose, antibodies raised experimentally or present in sera from patients who have autoimmune diseases as well as antibodies raised against nucleotide analogs have been applied. Formaldehyde or formaldehyde/ glutaraldehyde fixation and embedding in Lowicryl K4M or LR white resins provides high detection sensitivity. High spatial resolution is obtained by the use of gold-labelled secondary antibodies. With these techniques, DNA has been observed consistently in the dispersed and condensed chromatin of interphase nuclei, chromosomes of mitotic cells, and the mitochondria.
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Pavelka, M., Roth, J. (2010). Detection of Sites of NA Replication and of Interphase Chromosome domains. In: Functional Ultrastructure. Springer, Vienna. https://doi.org/10.1007/978-3-211-99390-3_5
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DOI: https://doi.org/10.1007/978-3-211-99390-3_5
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-99389-7
Online ISBN: 978-3-211-99390-3
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