Abstract
An extended classification of macrolides from the classical definition of Woodward presents a vast continuum of macrocyclic lactonic structures in which some molecules are mainly antibacterial (true macrolides) whereas others possess mainly immunosuppressant activity (FK 506, rapamycin) or antifungal activity with host cell inhibitory properties (bafilomycins, concannamycins). Traditional macrolide antibiotics are characterized by a 12- to 16-membered ring, macro-cyclic lactone nucleus, with few double-bonds, and substituted by several amino and/or neutral sugars. Over the past few decades, there has been continuous research into the development of new macrolide antibiotics by chemical modifications of the existing natural derivatives [1, 2]. This dynamic research has provided modern therapeutic agents, particularly the semi-synthetic derivatives of erythromycin A, either by adding new substituents, or introducing a nitrogen atom into the lactone (azalides), or, more recently, by withdrawing the L-cladinose at position 3 of the lactone ring and replacing it by a 3-keto function (ketolides). All these compounds display a substantially homogeneous antimicrobial spectrum and the capability to concentrate within host cells (the subject of this review). This property has been one major reason behind the search for new compounds targeting intracellular pathogens. Other possible consequences of the cellular accumulation of these drugs, particularly the modulation of various cell functions, are a further incentive for research in this field. A simplified chemical approach to the macrolide continuum is presented in Figure 1 [2, 3].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kirst HA (1991) New macrolides: expanded horizons for an old class of antibiotics. J AntimicrobChemother 28: 787–790
Bryskier A (1999) New research in macrolides and ketolides since 1997. Exp Opin Invest Drugs 8:1171–1194
Labro MT (1997) Effects of macrolides on leukocytes and inflammation. In: SH Zinner, LS Young, IF Acar, HC Neu (eds): Expanding indications for the new macrolides azalides and streptogramins,Marcel Dekker, New York, 101–116
Labro MT (1993) Intraphagocytic penetration of macrolide antibiotics. In: AJ Bryskier, J-P Butzler, HC Neu, PM Tulkens (eds): Macrolides: Chemistry,pharmacology and clinical uses. Arnette-Blackwell, Paris, 379–388
Labro MT (1997) Penetration intracellulaire des macrolides. Presse Med 26 (Supp1.11): 11–15
Vazifeh D, Abdelghaffar H, Labro MT (1997) Cellular accumulation of the new ketolide RU 64004 by human neutrophils: comparison with that of azithromycin and roxithromycin. Antimicrob Agents Chemother 41: 2099–2107
Vazifeh D, Preira A, Bryskier A, Labro MT (1998) Interactions between HMR 3647, a new ketolide, and human polymorphonuclear neutrophils. Antimicrob Agents Chemother 42: 1944–1951
Miossec-Bartoli C, Pilatre L, Peyron P, N’Diaye E-N, Collart-Dutilleul V, Maridonneau-Parini I, Diu-Hercend A (1999) The new ketolide HMR 3647 accumulates in the azurophilic granules of human polymorphonuclear cellsAntimicrob Agents Chemother 43: 2457–2462
Mtairag E M, Abdelghaffar H, Labro MT (1994) Investigation of dirithromycin and erythromy-cylamine uptake by human neutrophils in vitro. J Antimicrob Chemother 33: 523–536
Mtairag E.M, Abdelghaffar H, Douhet C, Labro MT (1995) Role of extracellular calcium in in vitro uptake and intraphagocytic location of macrolides. Antimicrob Agents Chemother 39: 1676–1682
Wildfeuer A, Reisert I, Laufen H (1993) Uptake and subcellular distribution of azithromycin in human phagocytic cells. Arzneim Forsch 43: 484–486
Vazifeh D, Labro MT (1999) Investigation of the uptake of HMR 3647, HMR 3004 and roxithromycin by myelomonocytic cell lines. Abstr 1929 In Program and abstracts of the 39th Intersci Conf Antimicrob Agents Chemother ASM Washington DC, p. 48
Raghoebar M, Van den Berg WB, Van Ginneken CAM (1987) Alteration of chloroquine accumulation in human polymorphonuclearleucocytes under inflammatory conditions. Int J Tissue React 9: 255–261
Laufen H, Wildfeuer A, Lach P (1990) Mechanism of azithromycin uptake in human polymorphonuclear leucocytes Arzneim Forsch 40: 686–689
Hand WL, King-Thompson N, Holman JW (1987) Entry of roxithromycin (RU 965), imipenem, cefotaxime, trimethoprim, and metronidazole into human polymorphonuclear leukocytes. Antimicrob Agents Chemother 31: 1553–1557
Labro MT, Abdelghaffar H, Vazifeh D, Bryskier A (1996) Roxithromycin uptake by human neutrophils is mediated by a protein kinase A-dependent mechanism. Abstr 110–018 In Program and abstracts of the 7 h Intern Congr Infect Dis. p. 278
Endicott JA, Ling V (1989) The biochemistry of P-glycoprotein-mediated multidrug resistance. Ann Rev Biochem 58: 137–171
Fardel 0, Lecureur V, Guillouzo A (1996) The P-glycoprotein multidrug transporter. Gen Pharmac 27: 1283–1291
Wakasugi H, Yano I, Ito T, Hashida T, Futami T, Nohara R, Sasayama S, Inui K (1998) Effect of clarithromycin on renal excretion of digoxin: interaction with P-glycoprotein. Clin Pharmacol Ther 64: 123–128
Arceci RJ, Stieglitz K, Bierer BE (1992) Immunosuppressant FK506 and rapamycin function as reversal agents of the multidrug-resistance phenotype. Blood. 80: 1528–36
Crosta L, Candiloro V, Meli M, Tolomeo M, Rausa L, Dusonchet (1994) Lacidipine and josamycin: two new multidrug resistance modulators. Anticancer Res 14: 2685–2690
Saeki T, Ueda K, Tanigawara Y, Hori R, Komano T (1993) Human P-glycoprotein transports cyclosporin A and FK506. J BiolChem 268: 6077–6080
Hand WL, Hand DL (1995) Influence of pentoxifylline and its derivatives on antibiotic uptake and superoxide generation by human phagocytic cells. Antimicrob Agents Chemother 39:1574–1579
Vazifeh D, Bryskier A, Labro MT (2000) Effect of proinflammatory cytokines on the interplay between roxithromycin, HMR 3647, or HMR 3004 and human polymorphonuclear neutrophils. Antimicrob Agents Chemother 44: 511–521
Bermudez LE, Inderlied C, Young LS (1991) Stimulation with cytokines enhances penetration of azithromycin into human macrophages. Antimicrob Agents Chemother 35: 2625–2629
Geerdes-Fenge HF, Goetschi B, Rau M, Borner K, Koeppe P, Wettich K, Lode H (1997) Comparative pharmacokinetics of dirithromycin and erythromycin in normal volunteers with special regard to accumulation in polymorphonuclear leukocytes and in saliva. Eur J Clin Pharmacol 53: 127–133
Ballow CH, Amsden GW, Highet VS, Forrest A (1998) Pharmacokinetics of oral azithromycin in serum, urine, polymorphonuclear leucocytes and inflammatory vs non-inflammatory skin blisters in healthy volunteers. Clin Drug Invest 15: 159–67
Baldwin DR, Wise R, Andrew JM, Ashby JP, Honeybourne D (1990) Azithromycin concentrations at the site of pulmonary infections. Eur Resp J 3: 886–90
Olsen KM, San Pedro GS, Gann LP, Gubbins PO, Halinski DM, Campbell GD jr (1996) Intrapulmonary pharmacokinetics of azithromycin in healthy volunteers given five oral doses. Antimicrob Agents Chemother 40: 2382–2385
Aubert J-D, Juillerat-Jeanneret L, Fioroni P, Dayer P, Plan P-A, Leuenberger P (1998) Function of alveolar macrophages after a 3-day course of azithromycin in healthy volunteers. Pulm Pharmacol Therapeut 11: 263–269
Stamler DA, Edelstein MAC, Edelstein PH (1994) Azithromycin pharmacokinetics and intracellular concentrations in Legionella pneumophila -infected and uninfected guinea pigs and their alveolar macrophages. Antimicrob Agents Chemother 38: 217–222
Gladue RP, Bright GM, Isaacson RE, Newborg MF (1985) In vitro and in vivo uptake of azithromycin (CP-52,993) by phagocytic cells: possible mechanism of delivery and release at sites of infection. Antimicrob Agents Chemother 33: 277–282
Frank MO, Sullivan GW, Carper HT, Mandell GL (1992) In vitro demonstration of transport and delivery of antibiotics by polymorphonuclear leukocytes. Antimicrob Agents Chemother 36: 2584–2588
Paul TR, Knight ST, Raulston JE, Wryck PB (1997) Delivery of azithromycin to Chlamydia trachomatis-infected polarized human endometrial epithelial cells by polymorphonuclear leucocytes. J Antimicrob Chemother 39: 623–630
Milisen WB, Girard AE (1993) Preferential concentrations of azithromycin in an infected mouse thigh model. J Antimicrob Chemother 31 (Suppl. E): 5–16
Fontan PA, Buzzola FR, Spinedi EG, Sordelli DO (1996) Haemophilus influenzae type b exoproducts induce chemotaxis and macrolide antibiotic release by human polymorphonuclear leukocytes. Chemother 42: 71–77.
Girard D, Bergeron JM, Milisen WB, Retsema JH (1993) Comparison of azithromycin, roxithromycin and cephalexin penetration kinetics in early and mature abscesses. J Antimicrob Chemother 31 (Suppl. E): 17–28
Labro MT (1993) Effects of macrolides on host natural defenses. In: AJ Bryskier, J-P Butzler, HC Neu, PM Tulkens (eds): Macrolides: Chemistry pharmacology and clinical uses AmetteBlackwell, Paris, 389–408
Labro MT (1998) Antiinflammatory activity of macrolides: a new therapeutic potential? J Antimicrob Chemother 41 (Suppl.B): 37–46
Labro MT (1998) Immunological effects of macrolides. Curr Opin Infect Dis 11: 681–688.
Abdelghaffar H, Vazifeh D, Labro MT (1997) Erythromycin A-derived macrolides modify the functional activities of human neutrophils by altering the phospholipase D-phosphatidate phosphohydrolase transduction pathway. J Immunol 159: 3995–4005
Hackstadt T (1998) The diverse habitats of obligate intracellular parasites. Curr Opin Microbiol 1: 82–87
Moulder JW (1985) Comparative biology of intracellular parasitism. Microbiol Rev 49: 298–337
Maurin M, Raoult D (1996) Optimum treatment of intracellular infections. Drugs. 52: 45–59
Butts JD (1994) Intracellular concentrations of antibacterial agents and related clinical implications. Clin Pharmacokinet 27: 63–84
Labro MT (1996) Intracellular bioactivity of macrolides. Clin Microbiol Infect 1 (Suppl.1): S24–S30
Anderson R, Van Rensburg CEJ, Joone G, Lukey PT (1987) An in vitro comparison of the intraphagocytic bioactivity of erythromycin and roxithromycin. J Antimicrob Chemother 20 (Suppl.B): 57–68
Labro MT, Amit C, Babin-Chevaye C, Hakim J (1986) Synergy between RU 28965 (roxithromycin) and human neutrophils for bactericidal activity in vitro. Antimicrob Agents Chemother 30: 137–142
Torigoe R (1993) The intracellular activity of ofloxacin and roxithromycin against Staphylococcus aureus phagocytosed in human neutrophils. Chemother 41: 955–62
Labro MT, Vazifeh D, Bryskier A (1999) Bactericidal activity of the ketolide H MR 3647 and roxithromycin in combination with human neutrophils. Abstr 1246 In Program and abstracts of the 39th Intersci Conf Antimicrob Agents Chemother ASM Washington DC, p. 258
Scaglione F, Demartini G, Dugnani S, Fraschini F (1993) A new model examining intracellular and extracellular activity of amoxicillin, azithromycin and clarithromycin in infected cells. Chemother 39: 416–423
Hoogeterp JJ, Mattie H, Van Furth R (1993) Activity of erythromycin and clindamycin in an experimental Staphylococcus aureus infection in normal and granulocytopenic mice. Scand J Infect Dis 25: 123–132
Stout JE, Arnold B, Yu VL (1998) Activity of azithromycin, clarithromycin, roxithromycin, dirithromycin, quinupristin/dalfopristin and erythromycin against Legionella species by intracellular susceptibility testing in HL-60 cells. J Antimicrob Chemother 41: 289–291
Donowitz GR, Earnhardt KI (1993) Azithromycin inhibition of intracellular Legionella micdadei.Antimicrob Agents Chemother 37: 2261–2264
Baltch AL, Smith RP, Franke MA, Michelsen PB (1998) Antibacterial effects of levofloxacin, erythromycin, and rifampin in a human monocyte system against Legionella pneumophila. Antimicrob Agents Chemother 42: 3153–3156
Ramirez JA (1993) Comparative study of the bactericidal activity of ampicillin/sulbactam and erythromycin against intracellular Legionella pneumophila. J Antimicrob Chemother 32: 93–99
Hammerschlag MR, Qumel KK, Roblin PM (1992) In vitro activities of azithromycin, clarithromycin, L-ofloxacin, and other antibiotics against Chlamydia pneumoniae. Antimicrob Agents Chemother 36: 1573–1574
Rastogi N, Labrousse V, Bryskier A (1995) Intracellular activities of roxithromycin used alone and in association with other drugs against Mycobacterium avium complex in human macrophages. Antimicrob Agents Chemother 39: 976–978
Bermudez LEM, Young LS (1988) Activities of amikacin, roxithromycin, and azithromycin alone or in combination with Tumor necrosis factor against Mycobacterium avium complex. Antimicrob Agents Chemother 32: 1149–1153
Skinner PS, Fumey SK, Jacobs MR, Klopman G, Ellner JJ, Orme IM (1994) A bone marrow-derived murine macrophage model for evaluating efficacy of antimycobacterial drugs under relevant physiologic conditions. Antimicrob Agents Chemother 38: 2557–2563.
Yajko DM, Sanders CA, Mady JJ, Cawthon VL, Hadley WK (1996) In vitro activities of rifabutin, azithromycin, ciprofloxacin, clarithromycin, clofazimine, ethambutol, and amikacin in combinations of two, three, and four drugs. Antimicrob Agents Chemother 40: 743–749.
Mor N, Heifets L (1993) MICs and MBCs of clarithromycin against Mycobacterium avium within human macrophages. Antimicrob Agents Chemother 37: 111–114.
Baradelli IE, Plosker GL, Metavish D (1993) Clarithromycin. A review of its pharmacologic properties and therapeutic use in mycobacterium avium intracellulare complex infection in patients with acquired immune deficiency syndrome. Drugs 46: 289–312
Lazard T, Perronne C, Cohen Y, Grosset J, Vilde JL, Pocidalo JJ (1993) Efficacy of granulocyte colony-stimulating factor and RU-40555 in combination with clarithromycin against Mycobacterium avium complex infection on C57 BL/6 miceAntimicrob. Agents Chemother 37: 692–695.
Blais J, Beauchamp D, Chamberland S (1994) Azithromycin uptake and intracellular accumulation by Toxoplasma gondii-infected macrophages. J Antimicrob Chemother. 34 : 371–382
Strickman D, Sheer T, Salata K, Hershey J, Dasch G, Kelly D, Kuschner R (1995) In vitro effectiveness of azithromycin against doxycycline-resistant and -susceptible Rickettsia tsutsugamushi, etiologic agent of scrub typhus . Antimicrob Agents Chemother 39: 2406–2410
Ives TJ, Manzewitsch P, Regnery RL, Butts JD. Kebede M (1997) In vitro susceptibilities of Bartonella henselae, B. quintana, B. elisabethae, Rickettsia rickettsii, R. conorii, R. akari, and R. prowazekii to macrolide antibiotics as determined by immunofluorescent-antibody analysis of infected vero cells monolayers Antimicrob Agents Chemother 41: 578–582
Nichterlein T, Kretschman M, Schadt A, Meyer A, Wildfeuer A, Laufen H, Hof H (1998) Reduced intracellular activity of antibiotics against Listeria monocytogenes in multidrug resistant cells. Int J Antimicrob Agents 10: 119–125
Ouadrhiri Y, Scorneaux B, Sibille Y, Tulkens PM (1999) Mechanism of the intracellular killing and modulation of antibiotic susceptibility of Listeria monocytogenes in THP-1 macrophages activated by gamma interferon. Antimicrob Agents Chemother 43: 1242–1251
Rakita RM, Jacques-Palaz K, Murray BE (1994) Intracellular activity of azithromycin against bacterial enteric pathogens. Antimicrob Agents Chemother 38: 1915–1921
Willot I, Scomeaux B, Tulkens PM (1993) Comparative intracellular activity of antibiotics against virulent L. monocytogenes and S. flexneri and their non-virulent mutants in a model of J774 macrophages. Abstr 769 In Program and abstracts of the 33rd Intersci Conf Antimicrob Agents Chemother
Abdelghaffar H, Mtairag EM, Labro MT (1994) Effect of dirithromycin and erythromycylamine on human neutrophil degranulation. Antimicrob Agents Chemother 38: 1548–1554
Frehel C, Offredo C, de Chastelier C (1997) The phagosomal environment protects virulent My cobacterium from killing and destruction by clarithromycin. Infect Immun 65: 2792–2802
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Basel AG
About this chapter
Cite this chapter
Labro, MT. (2002). Cellular accumulation of macrolide antibiotics. Intracellular bioactivity. In: Schönfeld, W., Kirst, H.A. (eds) Macrolide Antibiotics. Milestones in Drug Therapy MDT. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8105-0_4
Download citation
DOI: https://doi.org/10.1007/978-3-0348-8105-0_4
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9438-8
Online ISBN: 978-3-0348-8105-0
eBook Packages: Springer Book Archive