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Examination of the Globe. Technical Aspects

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Lee's Ophthalmic Histopathology
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Abstract

This chapter emphasises the methodology that is standard practice in the specialist ophthalmic pathology laboratory, but it also includes descriptions of the techniques that are recommended for investigation at the level necessary for presentation or publication. There is a description of techniques for handling the globe, the common macroscopic changes and normal histology. Information on special stains, immunohistochemistry and additional ancillary techniques is included but more detailed accounts are available in other pathology texts. The information included in this chapter should be underpinned by detailed background knowledge of clinical ophthalmology.

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References

  1. Wilson MW, Czechonska G, Finger PT, Rausen A, Hooper ME, Haik BG. Chemotherapy for eye cancer. Surv Ophthalmol. 2001;45:416–44.

    Article  CAS  Google Scholar 

  2. Sherwin JC, Hewitt AW, Ruddle JB, Mackey DA. Genetic isolates in ophthalmic diseases. Ophthalmic Genet. 2008;29:149–61.

    Article  CAS  Google Scholar 

  3. Raoof N, Rennie IG, Salvi SM, Sisley K, Caine A, Mudhar HS. What is the significance of vortex vein invasion in uveal melanoma? Eye (Lond). 2009;23:1661–7.

    Article  CAS  Google Scholar 

  4. Kuwabara T, Cogan DG. Studies of retinal vascular patterns. I. Normal architecture. Arch Ophthalmol. 1960;64:904–11.

    Article  CAS  Google Scholar 

  5. Laver NM, Robison G, Pfeffer BA. Novel procedure for isolating intact retinal vascular beds from diabetic humans and animal models. Invest Ophthalmol Vis Sci. 1993;34:2097–104.

    CAS  PubMed  Google Scholar 

  6. Olver JM, McCartney ACE. Anterior segment vascular casting. Eye. 1989;3:303–7.

    Google Scholar 

  7. Karcioglu ZA, Caldwell DR. Frozen section diagnosis in ophthalmic surgery. Surv Ophthalmol. 1984;28:323–32.

    Article  CAS  Google Scholar 

  8. Margo CE. Special histochemical stains in ocular pathology. Surv Ophthalmol. 1986;31:131–5.

    Article  CAS  Google Scholar 

  9. Devarajan B, Prakash L, Kannan TR, Abraham AA, Kim U, Muthukkapruppan V, et al. Targeted next generation sequencing of RB1 gene for the molecular diagnosis of retinoblastoma. BMC Cancer. 2015;15:320.

    Article  Google Scholar 

  10. Broadgate S, Yu J, Downes SM, Halford S. Unravelling the genetics of inherited retinal dystrophies: past, present and future. Prog Retin Eye Res. 2017;59:53–96.

    Article  CAS  Google Scholar 

  11. Ma L, Jakobiec F, Drya TP. A review of next-generation sequencing (NGS): applications to the diagnosis of ocular infectious diseases. Semin Ophthalmol. 2019;34:223–31.

    Article  CAS  Google Scholar 

  12. Reichstein D. Primary vitreoretinal lymphoma: an update on pathogenesis, diagnosis and treatment. Curr Opin Ophthalmol. 2016;27:177–84.

    Article  Google Scholar 

  13. Lavar NMV. Ocular cytopathology: a primer for the generalist. Semin Diagn Pathol. 2015;4:311–22.

    Article  Google Scholar 

  14. Seyed-Safi AG, Daniels JT. The limbus: structure and function. Exp Eye Res. 2020;197:108074.

    Article  CAS  Google Scholar 

  15. Marshall GE, Konstas AG, Lee WR. Collagens in ocular tissue. Br J Ophthalmol. 1993;77:515–24.

    Article  CAS  Google Scholar 

  16. Müller LJ, Pels L, Vrensen GFJM. Ultrastructural organisation of human corneal nerves. Invest Ophthalmol Vis Sci. 1996;37:476–8.

    PubMed  Google Scholar 

  17. Forrester JV, Xu H, Kuffova L, Dick AD, McMenamin PG. Dendritic cell physiology and function in the eye. Immunol Rev. 2010;234:282–304.

    Article  CAS  Google Scholar 

  18. Wilkerson CL, Syed NA, Fisher MR, Robinson NL, Wallow IHL, Albert DM. Melanocytes and iris colour. Arch Ophthalmol. 1996;114:437–42.

    Article  CAS  Google Scholar 

  19. Khalil AK, Kubota T, Tawara A, Inomata H. Ultrastructural age-related changes on the posterior iris surface. A possible relationship to the pathogenesis of exfoliation. Arch Ophthalmol. 1996;114:721–5.

    Article  CAS  Google Scholar 

  20. Taylor AW, Sehu KW, Lee WR, Williamson TW. Morphometric assessment of the central retinal artery and vein in the optic nerve head. Can J Ophthalmol. 1993;28:320–4.

    CAS  PubMed  Google Scholar 

  21. Kang MH, Balaratnasingam C, Yu PK, Morgan WH, McAllister IL, Cringle SJ, Yu D-Y. Morphometric characteristics of central retinal artery and vein endothelium in the normal human optic nerve head. Invest Ophthalmol Vis Sci. 2011;52:1359–67.

    Article  Google Scholar 

  22. May CA, Neuhuber W, Lutjec-Drecoll E. Immunohistochemical classification and functional morphology of human choroidal ganglion cells. Invest Ophthalmol Vis Sci. 2004;45:361–7.

    Article  Google Scholar 

  23. Mudhar HS, Ford AL, Ebrahimi KB, Farr R, Murray A. Intraocular choroidal extramedullary heamatopoiesis. Histopathology. 2005;46:694–6.

    Article  CAS  Google Scholar 

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Correspondence to Fiona Roberts .

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Roberts, F., Thum, C.K. (2021). Examination of the Globe. Technical Aspects. In: Lee's Ophthalmic Histopathology. Springer, Cham. https://doi.org/10.1007/978-3-030-76525-5_1

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  • DOI: https://doi.org/10.1007/978-3-030-76525-5_1

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-76524-8

  • Online ISBN: 978-3-030-76525-5

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