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Cellular Metabiotics and Metabolite Metabiotics

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METABIOTICS

Abstract

To provide a clearer picture of what modern techniques and technologies are used when developing metabiotics and launching them on the market of therapeutics and functional foods, let us describe how metabiotics were developed on the base of cell walls of the Lactobacillus reuteri DSM 17648 (Pylopass/Helinorm) and metabolites and signaling molecules on the base of the Bacillus subtilis No.3 probiotic strain (Bactistatin).

The chapter is written by Christine Lang (Technical University Berlin, Germany) and Kimmo Makinen (Novozymes S/A, Denmark).

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Bibliography

  • Ardatskaya MD Probiotics, prebiotics and metabiotics in the correction of microecological bowel disorders. Medical Council. 2015;3:94–99 (in Russian).

    Google Scholar 

  • Ardatskaya MD, Belmer SV, Dobritsa VP, Zakharenko SM, Lazebnik LB, Minushkin ON, Oreshko LS, Sitkin SI, Tkachenko EI, Suvorov AN, Khavkin AI, Shenderov BA. Colon dysbacteriosis (dysbiosis): modern state of the problem, comprehensive diagnosis and treatment correction. Experimental & Clinical Gastroenterology. 2015;117(5):13–50 (in Russian).

    Google Scholar 

  • Buckley M, Lacey S, Doolan A, Goodbody E, Seamans K. The effect of Lactobacillus reuteri supplementation in Helicobacter pylori infection: a placebo-controlled, single-blind study. BMC Nutrition. 2018;4:48. doi:https://doi.org/10.1186/s40795-018-0257-4.

    Article  PubMed  PubMed Central  Google Scholar 

  • Cain AM, Karpa KD. Clinical utility of probiotics in inflammatory bowel disease. Altern Ther Health Med. 2011;17(1):72–79.

    PubMed  Google Scholar 

  • Cats A, Kuipers EJ, Bosschaert MA, Pot RG, Vandenbroucke-Grauls CM, Kus-ters JG. Effect of frequent consumption of a Lactobacillus casei-containing milk drink in Helicobacter pylori-colonized subjects. Aliment Pharmacol Ther 2003;17:429–435.

    Article  CAS  PubMed  Google Scholar 

  • Cruchet S, Obregon MC, Salazar G, Diaz E, Gotteland M. Effect of the ingestion of a dietary product containing Lactobacillus johnsonii La1 on Helicobacter pylori colonization in children. Nutrition. 2003 Sep;19(9):716–21.

    Article  PubMed  Google Scholar 

  • Emara MH, Mohamed SY, Abdel-Aziz HR. Lactobacillus reuteri in management of Helicobacter pylori infection in dyspeptic patients: a double-blind placebo-controlled randomized clinical trial. Therap Adv Gastroenterol. 2014;7(1):4–13. doi:https://doi.org/10.1177/1756283X13503514.

    Article  PubMed  PubMed Central  Google Scholar 

  • Francavilla R, Lionetti E, Castellaneta S, Magistà A, Maurogiovanni G et al. Inhibition of Helicobacter pylori infection in humans by Lactobacillus reuteri ATCC 55730 and effect on eradication therapy: a pilot study. Helicobacter. 2008;13:127–134. doi:https://doi.org/10.1111/j.1523-5378.2008.00593.x.

    Article  PubMed  Google Scholar 

  • Holz C, Busjahn A, Mehling H, Arya S, Boettner M, Habibi H, Lang C. Significant Reduction in Helicobacter pylori Load in Humans with Non-viable Lactobacillus reuteri. Probiotics Antimicrob Proteins. 2015;7(2):91–100. doi:https://doi.org/10.1007/s12602-014-9181-3.

    Article  PubMed  Google Scholar 

  • Ilinskaya ON, Ulyanova VV, Yarullina DR, Gataullin IG. Secretome of intestinal Bacilli; a nature guard against pathologies. Front Microbiol. 2017;8:1666. doi:https://doi.org/10.3389/fmicb.2017.01666.

    Article  PubMed  PubMed Central  Google Scholar 

  • Khoder G, Al-Menhali AA, Al-Yassir F, Karam SM. Potential role of probiotics in the management of gastric ulcer. Exp Ther Med. 2016;12(1):3–17. doi:https://doi.org/10.3892/etm.2016.3293.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim Y, Edwards N, Fenselau C. Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis. Clin Proteomics. 2016;13:6. doi:https://doi.org/10.1186/s12014-016-9107-z.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lefevre M, Racedo SM, Ripert G, Housez B, Cazaubiel M et al. Probiotic strain Bacillus subtilis CU1 stimulates immune system of elderly during common infectious disease period: a randomized, double-blind placebo-controlled study. Immun Ageing. 2015;12:24. doi:https://doi.org/10.1186/s12979-015-0051-y.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lesbros-Pantoflickova D, Corthesy-Theulaz I, Blum AL. Helicobacter pylori and probiotics. J Nutr. 2007;137(3 Suppl 2):812S–818S. doi:https://doi.org/10.1093/jn/137.3.812S.

    Article  CAS  PubMed  Google Scholar 

  • Linsalata M, Russo F, Berloco P, Caruso ML, Matteo GD, Cifone MG, Simone CD, Ierardi E, Di Leo A. The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa. Helicobacter 2004;9:165–172. doi:https://doi.org/10.1111/j.1083-4389.2004.00214.x.

    Article  CAS  PubMed  Google Scholar 

  • Lionetti E, Miniello V, Castellaneta S, Magistá A, de Canio A, Maurogiovanni G. et al. Lactobacillus reuteri therapy to reduce side-effects during anti-Helicobacter pylori treatment in children: a randomized placebo controlled trial, Aliment Pharmacol Ther 2006;24:1461–1468. doi:https://doi.org/10.1111/j.1365-2036.2006.03145.x.

    Article  CAS  PubMed  Google Scholar 

  • Malfertheiner P, Megraud F, O’Morain CA, Gisbert JP, Kuipers EJ et al. Management of Helicobacter pylori infection-the Maastricht V / Florence Consensus Report. Gut. 2017;66(1):6–30. doi:https://doi.org/10.1136/gutjnl-2016-312288.

    Article  CAS  PubMed  Google Scholar 

  • Mehling H, Busjahn A. Non-viable Lactobacillus reuteri DSMZ17648 (Pylopassâ„¢) as a new approach to Helicobacter pylori control in humans. Nutrients. 2013;5(8):3062–3073. doi:https://doi.org/10.3390/nu5083062.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Michetti P, Dorta G, Wiesel PH, Brassart D, Verdu E, Herranz M, Felley C, Porta N, Rouvet M, Blum AL, Corthésy-Theulaz I. Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Digestion 1999;60:203–209. doi:https://doi.org/10.1159/000007660.

    Article  CAS  PubMed  Google Scholar 

  • Minushkin ON, Ardatskaya MD, Sergeev AV, Volkov MYu, Sinitsa AV. Experience in the use of probiotic «Baktistatin» in the treatment of chronic pancreatitis. Pharmacy. 2006;3:39–43 (in Russian).

    Google Scholar 

  • Mukai T, Asasaka T, Sato E, Mori K, Matsumoto M, Ohori H. Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri. FEMS Immunol Med Microbiol. 2002;32(2):105–10. doi:https://doi.org/10.1111/j.1574-695X.2002.tb00541.x.

    Article  CAS  PubMed  Google Scholar 

  • Oleskin AV, El’-Registan GI, Shenderov BA. Role of neuromediators in the functioning of the human microbiota: «business talks» among microorganisms and the microbiota-host dialogue. Microbiology. 2016;85(1):1–22.

    Article  CAS  Google Scholar 

  • Olmos J, Paniagua-Michel J. Bacillus subtilis A Potential Probiotic Bacterium to Formulate Functional Feeds for Aquaculture. J Microb Biochem Technol. 2014;6:361–365. doi:https://doi.org/10.4172/1948-5948.1000169.

    Article  CAS  Google Scholar 

  • Perez KJ, Viana JD, Lopes FC, Pereira JQ, Dos Santos DM et al. Bacillus spp isolated from puba as a source of biosurfactants and antimicrobial lipopeptides. Front Microbiol. 2017;8:61. doi:https://doi.org/10.3389/fmicb.2017.00061.

    Article  PubMed  PubMed Central  Google Scholar 

  • Phister TG, O’Sullivan DJ, McKay LL. Identification of bacilysin, chlorotetaine, and iturin a produced by Bacillus sp. strain CS93 isolated from Pozol, a Mexican fermented maize dough. Appl Environm Microbiol. 2004;70(1):631–634. doi:https://doi.org/10.1128/AEM.70.1.631-634.2004.

    Article  CAS  Google Scholar 

  • Quintana VM, Torres NI, Wachsman MB, Sinko PJ, Castilla V, Chikindas M. Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin. J Appl Microbiol. 2014;117(5):1253–1259. doi:https://doi.org/10.1111/jam.12618.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Scaccianoce G, Zullo A, Hassan C, Gentili F, Cristofari F et al. Triple therapies plus different probiotics for Helicobacter pylori eradication. Eur Rev Med Pharmacol Sci. 2008;12(4):251–256.

    CAS  PubMed  Google Scholar 

  • Sebastian AP, Keerthi TR. Immunomodulatory effect of probiotic strain Bacillus subtilis MBTU PBBM1 spores in Balb/C mice. Int J Eng Tech Res. 2014;2(11):258–260.

    Google Scholar 

  • Shenderov BA, Tkachenko EI, Lazebnik LB, Ardatskaya MD, Sinitsa AV, Zakharchenko MM. Metabiotics-novel technology of protective and treatment of diseases associated with microecological imbalance in human beings. Experimental & Clinical Gastroenterology. 2018a;151:83–92 (in Russian).

    Google Scholar 

  • Shenderov BA, Yudin SM, Shevyreva MP, Boyko EA. The scientific backgrounds for the creation of a microecological cryopreservation of human resources. Hygiene and Sanitation. 2018b;97(5):396–398 (in Russian).

    Article  Google Scholar 

  • Shenderov BA. Metabiotics — novel prophylactic technology of diseases associated with microecological imbalance of human being. Journal of Restorative Medicine & Rehabilitation. 2017;4:40–49 (in Russian).

    Google Scholar 

  • Shenderov BA. Role of mitochondria in preventive, restorative and sport medicine. Journal of Restorative Medicine & Rehabilitation. 2018;1:21–31 (in Russian).

    Google Scholar 

  • Sorokulova I. Modern status and perspectives of Bacillus bacteria as Probiotics. J Prob Health. 2013;1(4):e106. doi:https://doi.org/10.4172/2329-8901.1000e106.

    Article  Google Scholar 

  • Suerbaum S, Michetti P. Helicobacter pylori infection. N Engl J Med. 2002;347(15):1175–1186. doi:https://doi.org/10.1056/NEJMra020542.

    Article  CAS  PubMed  Google Scholar 

  • Sumi CD, Yang BW, Yeo IC, Hahm YT. Antimicrobial peptides of the genus Bacillus. A new era for antibiotics. Can J Microbiol. 2015;61(2):93–103. doi:https://doi.org/10.1139/cjm-2014-0613.

    Article  CAS  PubMed  Google Scholar 

  • Suva MA, Sureja VP, Kheni DB. Novel insight on probiotic Bacillus subtilis: Mechanism of action and clinical applications. J Curr Res Sci Med. 2016;2:65–72. doi:https://doi.org/10.4103/2455-3069.198381.

    Article  Google Scholar 

  • Viganò L, Cinzia C, Oliveira A, Guerrieri P. Helicobacter pylori: Is there an Association with Oral Pathologies? A Traditional Review. Acta Scientific Microbiology. 2018;1(9):32–39.

    Google Scholar 

  • Volkov MYu, Tkachenko EI, Vorobeichikov EV, Sinitsa AV. Bacillus subtilis metabolites as a novel promising probiotic preparations. Zh Mikrobiol (Moscow). 2007;2:75–80 (in Russian).

    Google Scholar 

  • Volkov MYu, Zakharchenko MM, Sinitsa AV, Tkachenko EA, Uspenskiy YuP. Novel functional food products based on biotechnology. Military medical journal. 2006;5:69–70 (in Russian).

    Google Scholar 

  • Volkov MYu. Toxicological studies of a new probiotic based on metabolites of bacilli and natural zeolite. News of higher educational institutions. North Caucasus region. Natural science. Special issue. 2008;23–26 (in Russian).

    Google Scholar 

  • Vorobeichikov EV, Stepanov AV, Volkov MYu, Vasilenko AZh, Ponomarenko VM, Sinitsa AV. Immunotropic effects of probiotic complex Bactistatin on the background of antibiotics. Antibiotics and chemotherapy. 2008;53(1):3–9 (in Russian).

    CAS  PubMed  Google Scholar 

  • Wang Y, Wu Y, Wang Y, Xu H, Mei X et al. Antioxidant Properties of Probiotic Bacteria. Nutrients. 2017a;9(5):521. doi:https://doi.org/10.3390/nu9050521.

    Article  CAS  PubMed Central  Google Scholar 

  • Wang ZJ, Chen XF, Zhang ZX, Li YC, Deng J, Tu J et al. Effects of anti-Helicobacter pylori concomitant therapy and probiotic supplementation on the throat and gut microbiota in humans. Microb Pathogenesis. 2017b;109:156–161. doi:https://doi.org/10.1016/j.micpath.2017.05.035.

    Article  CAS  Google Scholar 

  • Zarrilli R, Ricci V, Romano M. Molecular response of gastric epithelial cells to Helicobacter pylori-induced cell damage. Cell Microbiol. 1999;1(2):93–99.

    Article  CAS  PubMed  Google Scholar 

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Shenderov, B.A., Sinitsa, A.V., Zakharchenko, M.M., Lang, C. (2020). Cellular Metabiotics and Metabolite Metabiotics. In: METABIOTICS. Springer, Cham. https://doi.org/10.1007/978-3-030-34167-1_14

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