Skip to main content

Microbial Enzymes in Food Processing

  • Chapter
  • First Online:
Biocatalysis

Abstract

Enzymes are of great significance in the food processing industry as they are highly specific in their action on substrates, work under mild conditions, minimal by-product formation, improve yield, and pose fewer environmental problems. Enzymes are efficient bio-catalysts for an array of reactions and have been obtained from various sources such as animals, plants and microorganisms. However, microbial enzymes are preferred over other sources due to the ease of cultivation, handling, manipulation, higher efficiency and stability. They are considered as potentially interesting candidates for industrial applications because they can be cultured in substantial quantities in a short span of time by fermentation on incredibly diverse, easy to produce, a wide range of inexpensive and readily available carbon and nitrogen sources.

Microbes offer an important and vast reservoir of these biocatalysts for carrying out specific biochemical reactions. History of microbial enzymes reveals that many beneficial products like foods, beverages and antibiotics were produced using microorganisms. In the recent years, with the advent of metagenomics using low-cost high-throughput sequencing technology, it has become possible to characterize the variety of microbial species from nature. In addition, recombinant DNA techniques have made it possible to develop highly efficient expression systems that can lead to further enhancement and refinement of catalytic properties of enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Ahmad Z, Butt MS, Ahmed A, Riaz M, Sabir SM, Farooq U, Rehman FU (2014) Effect of Aspergillus niger xylanase on dough characteristics and bread quality attributes. J Food Sci Technol 51(10):2445–2453

    CAS  PubMed  Google Scholar 

  • Allen MJ, Fang TY, Li Y, Liu HL, Chen HM, Coutinho P, Honzatko R, Ford C (2003) Protein engineering of glucoamylase to increase pH optimum, substrate specificity and thermostability. U.S. Patent No. 6,537,792. Washington, DC: U.S. Patent and Trademark Office

    Google Scholar 

  • Anbu P, Gopinath SC, Chaulagain BP, Tang TH, Citartan M (2015) Microbial enzymes and their applications in industries and medicine 2014. Biomed Res Int 2015:816419

    PubMed  PubMed Central  Google Scholar 

  • Andualema B, Gessesse A (2012) Microbial lipases and their industrial applications. Biotechnology 11(3):100–118

    CAS  Google Scholar 

  • Aravindan R, Anbumathi P, Viruthagiri T (2007) Lipase applications in food industry. Indian J Biotechnol 6:141–158

    CAS  Google Scholar 

  • Arihara K (2006) Strategies for designing novel functional meat products. Meat Sci 74(1):219–229

    CAS  PubMed  Google Scholar 

  • Avendano KA, Anguiano M, Lopez CE, Montanez LE, Sifuentes L, Balagurusamy N (2016) Microbial enzymes applications in food processing. Agro Food Ind Hi Tech 27:63–67

    CAS  Google Scholar 

  • Bessler C, Schmitt J, Maurer KH, Schmid RD (2003) Directed evolution of a bacterial α-amylase: toward enhanced pH-performance and higher specific activity. Protein Sci 12(10):2141–2149

    CAS  PubMed  PubMed Central  Google Scholar 

  • Borch K, Erlandsen L, Jensen M (2006) Treatment of dough with a lipoxygenase and a lipolytic enzyme. U.S. Patent Application No. 10/528,330

    Google Scholar 

  • Cha JAEHO, Batt CA (1998) Lowering the pH optimum of D-xylose isomerase: the effect of mutations of the negatively charged residues. Mol Cells 8(4):374–382

    CAS  PubMed  Google Scholar 

  • Chapman-Smith A, Cronan JE Jr (1999) The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity. Trends Biochem Sci 24(9):359–363

    CAS  PubMed  Google Scholar 

  • Chaurasia SP, Bhandaria K, Sharmaa A, Dalai AK (2016) A review on lipase catalysed synthesis of DHA rich glyceride from fish oils. Int J Res Sci Innov 3:9–19

    Google Scholar 

  • Dahiya S (2016) Industrial application of phytases. Int J Appl Res 2:95–98

    Google Scholar 

  • Danielsen S, Lundqvist H (2008) Bacterial alpha-amylase variants. WO Patent 825, 2008

    Google Scholar 

  • Declerck N, Machius M, Wiegand G, Huber R, Gaillardin C (2000) Probing structural determinants specifying high thermostability in Bacillus licheniformis α-amylase1. J Mol Biol 301(4):1041–1057

    CAS  PubMed  Google Scholar 

  • Demain AL, Adrio JL (2008) Contributions of microorganisms to industrial biology. Mol Biotechnol 38(1):41

    CAS  PubMed  Google Scholar 

  • Duan X, Chen J, Wu J (2013) Improving the thermostability and catalytic efficiency of Bacillus deramificans pullulanase by site-directed mutagenesis. Appl Environ Microbiol 79(13):4072–4077

    CAS  PubMed  PubMed Central  Google Scholar 

  • Dwevedi A, Kayastha AM (2009) Stabilization of β-galactosidase (from peas) by immobilization onto Amberlite MB-150 beads and its application in lactose hydrolysis. J Agric Food Chem 57(2):682–688

    CAS  PubMed  Google Scholar 

  • Ellaiah P, Adinarayana K, Bhavani Y, Padmaja P, Srinivasulu B (2002) Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species. Process Biochem 38(4):615–620

    CAS  Google Scholar 

  • England G, Kolkman M, Miller B, Vroemen C (2013) Pullulanase variants with increased productivity. U.S. Patent No. 8,354,101. Washington, DC: U.S. Patent and Trademark Office

    Google Scholar 

  • Faisal M, Islami SNE, Islam MN, Kamal M, Khan MNA (2015) Study on microbial and physical changes in fish sauce during fermentation. Res Agric Livestock Fish 2(2):375–383

    Google Scholar 

  • Fernandes P (2010) Enzymes in food processing: a condensed overview on strategies for better biocatalysts. Enzyme Res 2010:1–19. https://doi.org/10.4061/2010/862537

    Article  CAS  Google Scholar 

  • Fernandes P (2016) Enzymes in fish and seafood processing. Front Bioeng Biotechnol 4:59

    PubMed  PubMed Central  Google Scholar 

  • Flamm EL (1991) How FDA approved chymosin: a case history. Nat Biotechnol 9(4):349

    CAS  Google Scholar 

  • Food Enzymes Market by Type (Carbohydrase, Protease, Lipase), Application (Beverages, Bakery Products, Dairy Products, Confectionery Products, Processed Foods), Formulation (Lyophilized Powder and Liquid), Source, and Region – Global Forecast to 2023. https://www.marketsandmarkets.com/Market-Reports/food-enzymes-market-800.html

  • Food Enzymes Market: Global Trends and Forecasts to 2018. http://www.researchandmarkets.com/research/3xgvqh/food_enzymes

  • Garg G, Singh A, Kaur A, Singh R, Kaur J, Mahajan R (2016) Microbial pectinases: an ecofriendly tool of nature for industries. 3 Biotech 6(1):47

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ghaly AE, Ramakrishnan VV, Brooks MS, Budge SM, Dave D (2013) Fish processing wastes as a potential source of proteins. Amino acids and oils: a critical review. J Microb Biochem Technol 5(4):107–129

    Google Scholar 

  • Ghollasi M, Ghanbari-Safari M, Khajeh K (2013) Improvement of thermal stability of a mutagenised α-amylase by manipulation of the calcium-binding site. Enzym Microb Technol 53(6–7):406–413

    CAS  Google Scholar 

  • Goswami GK, Rawat S (2015) Microbial Xylanase and their applications – a review. Int J Curr Res Acad Rev 3(6):436–450

    CAS  Google Scholar 

  • Guerrand D (2018) Economics of food and feed enzymes: status and prospectives. In: Enzymes in human and animal nutrition, pp 487–514

    Google Scholar 

  • Gunders D (2012) Wasted: how America is losing up to 40 percent of its food from farm to fork to landfill. Natural Resources Defense Council, 26

    Google Scholar 

  • Hii SL, Tan JS, Ling TC, Ariff AB (2012) Pullulanase: role in starch hydrolysis and potential industrial applications. Enzyme Res 2012:921362

    PubMed  PubMed Central  Google Scholar 

  • Husain Q (2016) Magnetic nanoparticles as a tool for the immobilization/stabilization of hydrolases and their applications: an overview. Biointerfaces Res Appl Chem 6(6):1585–1606

    CAS  Google Scholar 

  • Husain Q (2017a) Nanomaterials as novel support for the immobilization of amylolytic enzymes and their applications: a review. DeGruyter Biocatal 3(1):37–53

    Google Scholar 

  • Husain Q (2017b) Nanomaterials immobilized cellulolytic enzymes and their industrial applications: a literature review. JSM Biochem Mol Biol 4(3):1029

    Google Scholar 

  • Husain Q (2017c) Nanosupport bound lipases their stability and applications. Biointerfaces Res Appl Chem 7(6):2194–2216

    CAS  Google Scholar 

  • Husain Q (2018) Nanocarriers immobilized proteases and their industrial applications: an overview. J Nanosci Nanotechnol 18(1):486–499

    CAS  PubMed  Google Scholar 

  • Hussein L, Elsayed S, Foda S (1989) Reduction of lactose in milk by purified lactase produced by Kluyveromyces lactis. J Food Prot 52(1):30–34

    CAS  PubMed  Google Scholar 

  • Igarashi K, Ozawa T, Ikawa-Kitayama K, Hayashi Y, Araki H, Endo K, Hagihara H, Ozaki K, Kawai S, Ito S (1999) Thermostabilization by proline substitution in an alkaline, liquefying α-amylase from Bacillus sp. strain KSM-1378. Biosci Biotechnol Biochem 63(9):1535–1540

    CAS  PubMed  Google Scholar 

  • Karimäki J, Parkkinen T, Santa H, Pastinen O, Leisola M, Rouvinen J, Turunen O (2004) Engineering the substrate specificity of xylose isomerase. Protein Eng Des Sel 17(12):861–869

    PubMed  Google Scholar 

  • Kieliszek M, Misiewicz A (2014) Microbial transglutaminase and its application in the food industry. A review. Folia Microbiol 59(3):241–250

    CAS  Google Scholar 

  • Kim MS, Lei XG (2008) Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR. Appl Microbiol Biotechnol 79(1):69–75

    CAS  PubMed  Google Scholar 

  • Kirk O, Borchert TV, Fuglsang CC (2002) Industrial enzyme applications. Curr Opin Biotechnol 13(4):345–351

    CAS  PubMed  Google Scholar 

  • Kumar S (2015) Role of enzymes in fruit juice processing and its quality enhancement. Health 6(6):114–124

    CAS  Google Scholar 

  • Kumar P, Suneetha V (2016) Microbial pectinases: wonderful enzymes in fruit juice clarification. Int J MediPharm Res 2:119–127

    Google Scholar 

  • Maróstica MR, Pastore GM (2010) Some nutritional, technological and environmental advances in the use of enzymes in meat products. Enzyme Res 2010:480923

    PubMed  PubMed Central  Google Scholar 

  • McDaniel A, Fuchs E, Liu Y, Ford C (2008) Directed evolution of Aspergillus niger glucoamylase to increase thermostability. Microb Biotechnol 1(6):523–531

    CAS  PubMed  PubMed Central  Google Scholar 

  • McSweeney PL, Sousa MJ (2000) Biochemical pathways for the production of flavour compounds in cheeses during ripening: a review. Lait 80(3):293–324

    CAS  Google Scholar 

  • Miguel ÂSM, Martins-Meyer TS, da Costa Figueiredo ÉV, Lobo BWP, Dellamora-Ortiz GM (2013) Enzymes in bakery: current and future trends. In: Muzzalupo I (ed) Food industry. InTech, UK

    Google Scholar 

  • Neelakantan S, Mohanty AK, Kaushik JK (1999) Production and use of microbial enzymes for dairy processing. Curr Sci 77(1):143–148

    CAS  Google Scholar 

  • Nelson DL, Cox MM (2004) Lehninger principles of biochemistry, 4th edn. WH Freeman, New York

    Google Scholar 

  • Nielsen PH, Malmos H, Damhus T, Diderichsen B, Nielsen HK, Simonsen M, SchiV HE, Oestergaard A, Olsen HS, Eigtved P, Nielsen TK (1994) Enzyme applications, industrial. In: Kirk-Othmere Encyclopedia of chemical technology. Wiley, New York

    Google Scholar 

  • Novozymes (https://report2016.novozymes.com/our-business/trends)

  • Oelofse SH, Nahman A (2013) Estimating the magnitude of food waste generated in South Africa. Waste Manag Res 31(1):80–86

    PubMed  Google Scholar 

  • Oh DK, Oh HJ, Kim HJ, Cheon J, Kim P (2006) Modification of optimal pH in L-arabinose isomerase from Geobacillus stearothermophilus for D-galactose isomerization. J Mol Catal B Enzym 43(1–4):108–112

    CAS  Google Scholar 

  • Prakash O, Jaiswal N (2010) Alpha-Amylase: an ideal representative of thermostable enzymes. Appl Biochem Biotechnol 160(8):2401–2414

    PubMed  Google Scholar 

  • Qureshi N, Blaschek HP (2005) Butanol production from agricultural biomass. In: Food biotechnology. CRC, Boca Raton, FL, pp 541–565

    Google Scholar 

  • Raveendran S, Parameswaran B (2018) Applications of microbial enzymes in food industry. Food Technol Biotechnol 56(1):16–30

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rea MC, Franz CMAP, Holzapfel WH, Cogan TM (2004) Development of enterococci and production of tyramine during the manufacture and ripening of Cheddar cheese. Ir J Agric Food Res 43(2):247–258

    CAS  Google Scholar 

  • Rubin-Pitel SB, Zhao H (2006) Recent advances in biocatalysis by directed enzyme evolution. Comb Chem High Throughput Screen 9(4):247–257

    CAS  PubMed  Google Scholar 

  • Shammet KM, Brown RJ, McMahon DJ (1992) Proteolytic activity of some milk-clotting enzymes on κ-Casein1. J Dairy Sci 75(6):1373–1379

    CAS  Google Scholar 

  • Singh R, Kumar M, Mittal A, Mehta PK (2016a) Microbial enzymes: industrial progress in 21st century. 3 Biotech 6:174

    PubMed  PubMed Central  Google Scholar 

  • Singh R, Mittal A, Kumar M, Mehta P (2016b) Amylases: a note on current applications. Int Res J Biol Sci 5(11):27–32

    Google Scholar 

  • Singh R, Mittal A, Kumar M, Mehta PK (2016c) Microbial proteases in commercial applications. J Pharm Chem Biol Sci 4:365–374

    CAS  Google Scholar 

  • Sridevi DV, Ramanujam AR (2012) Performance of mixture of vegetable wastes with high cabohydrate content in anaerobic digestion process. Int J Environ Sci 3(1):181

    Google Scholar 

  • Sriprapundh D, Vieille C, Zeikus JG (2003) Directed evolution of Thermotoga neapolitana xylose isomerase: high activity on glucose at low temperature and low pH. Protein Eng 16(9):683–690

    CAS  PubMed  Google Scholar 

  • Starch Derivatives Market by Type (Maltodextrin, Cyclodextrin, Glucose Syrup, Spray Dried Starch, Hydrolysates), Application (F & B, Feed, Paper, Cosmetics, Pharmaceutical), Function (Emulsifying, Binding, Stabilizing, Thickening), & by Region – Global Trends & Forecast to 2020. http://www.marketsandmarkets.com/Market-Reports/starch-derivatives-market-116279237.html

  • Suresh PV, Nidheesh T, Pal GV (2015) Enzymes in seafood processing. In: Chandrasekaran M (ed) Enzymes in food and beverage processing. CRC, Boca Raton, FL, pp 354–377

    Google Scholar 

  • Tang WL, Zhao H (2009) Industrial biotechnology: tools and applications. Biotechnol J Healthc Nutr Technol 4(12):1725–1739

    CAS  Google Scholar 

  • Tarhan L (1995) Use of immobilised catalase to remove H2O2 used in the sterilisation of milk. Process Biochem 30(7):623–628

    Google Scholar 

  • Tomschy A, Brugger R, Lehmann M, Svendsen A, Vogel K, Kostrewa D, Lassen SF, Burger D, Kronenberger A, van Loon AP, Pasamontes L (2002) Engineering of phytase for improved activity at low pH. Appl Environ Microbiol 68(4):1907–1913

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ueda T, Masumoto K, Ishibashi R, So T, Imoto T (2000) Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme. Protein Eng 13(3):193–196

    CAS  PubMed  Google Scholar 

  • Wang Y, Fuchs E, da Silva R, McDaniel A, Seibel J, Ford C (2006) Improvement of Aspergillus niger glucoamylase thermostability by directed evolution. Starch-Stärke 58(10):501–508

    CAS  Google Scholar 

  • Wong DW, Batt SB, Lee CC, Robertson GH (2004) High-activity barley α-amylase by directed evolution. Protein J 23(7):453–460

    CAS  PubMed  Google Scholar 

  • World Enzymes (2011) World food & beverage enzyme demand by market (million dollars). Freedonia Group, Cleveland, OH. https://www.freedoniagroup.com/industry-study/world-enzymes-2824.htm

  • Wu Y, Yuan S, Chen S, Wu D, Chen J, Wu J (2013) Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase. Food Chem 138(2–3):1588–1595

    CAS  PubMed  Google Scholar 

  • Zheng M, Du G, Guo W, Chen J (2001) A temperature-shift strategy in batch microbial transglutaminase fermentation. Process Biochem 36(6):525–530

    CAS  Google Scholar 

  • Zhou C, Xue Y, Ma Y (2015) Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11. BMC Biotechnol 15(1):97

    PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Praveen Kumar Mehta .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Mehta, P.K., Sehgal, S. (2019). Microbial Enzymes in Food Processing. In: Husain, Q., Ullah, M. (eds) Biocatalysis. Springer, Cham. https://doi.org/10.1007/978-3-030-25023-2_13

Download citation

Publish with us

Policies and ethics