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Outer Membrane Porins

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Bacterial Cell Walls and Membranes

Part of the book series: Subcellular Biochemistry ((SCBI,volume 92))

Abstract

The transport of small molecules across membranes is essential for the import of nutrients and other energy sources into the cell and, for the export of waste and other potentially harmful byproducts out of the cell. While hydrophobic molecules are permeable to membranes, ions and other small polar molecules require transport via specialized membrane transport proteins . The two major classes of membrane transport proteins are transporters and channels. With our focus here on porins—major class of non-specific diffusion channel proteins , we will highlight some recent structural biology reports and functional assays that have substantially contributed to our understanding of the mechanism that mediates uptake of small molecules, including antibiotics, across the outer membrane of Enterobacteriaceae . We will also review advances in the regulation of porin expression and porin biogenesis and discuss these pathways as new therapeutic targets.

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Acknowledgements

We are especially grateful to Anne Davin-Regli, Julia Vergalli and Jean-Michel Bolla (UMR_MD1, Inserm U1261) and Matthieu Réfrégiers (Disco Beamline, Synchrotron Soleil) for sharing fruitful discussions.

The research leading to these results was conducted as part of the TRANSLOCATION consortium, and it has received support from the Innovative Medicines Initiatives Joint Undertaking under Grant Agreement n°115525, resources which are composed of financial contribution from the European Union’s seventh framework program (FP7/2007–2013) and EFPIA companies in kind contribution. This work was also supported by Aix-Marseille Univ. and Service de Santé des Armées, and by Soleil programs.

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  1. UMR_MD1, Inserm U1261, IRBA, Membranes et Cibles Thérapeutiques, Facultés de Médecine et de Pharmacie, Aix-Marseille Université, Marseille, France

    Muriel Masi & Jean-Marie Pagès

  2. Department of Life Sciences and Chemistry, Jacobs University, Bremen, Germany

    Mathias Winterhalter

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  1. Muriel Masi
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  2. Mathias Winterhalter
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  3. Jean-Marie Pagès
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Correspondence to Jean-Marie Pagès .

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  1. Inst. of Microbiology, University of Hohenheim, Stuttgart, Germany

    Andreas Kuhn

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Masi, M., Winterhalter, M., Pagès, JM. (2019). Outer Membrane Porins. In: Kuhn, A. (eds) Bacterial Cell Walls and Membranes . Subcellular Biochemistry, vol 92. Springer, Cham. https://doi.org/10.1007/978-3-030-18768-2_4

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