Abstract
The facts about the effects of high frequency electromagnetic fields (HF EMF) on human health are often controversial and incomplete. For this reason, the aim of this study was to examine impact of HF EMF on process of angiogenesis in eight rat organs of: pancreas, kidney, thyroid, liver, cerebrum, thymus, gastric gland and spleen. Wistar strain rats were exposed to HF EM fields with the following characteristics: 1.9 GHz frequency, 0.24 A/m intensity, electric field strength of 4.79 V/m, and SAR (specific absorption rate) value of 2.0 W/m2. Exposure time was 7 h per day, 5 days per week, over the course of 60 days. Our experiment was conducted on a total of 20 male rats divided randomly into two equal groups: one group of animals was exposed to HF EM fields as described above whereas, the other group of animals was not exposed to any HF EM fields. In our study histological and stereological analysis shows the results that volume density and number of endothelial cells of blood vessels increased with statistical significance in all organs of rats that were exposed to the HF EMF compared to the unexposed group. Increase in volume and number of endothelial cells in analyzed organs indicates the process of angiogenesis induced by HF EM fields, which can be used for therapeutic protocols.
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References
Gojković, I., Paraš, S., Gajanin, R., Matavulj, M.: The influence of extremely low-frequency electromagnetic field on the basal ganglia structures of the rat brain. Proc. Nat. Sci. Matica Srpska. 121, 27–38 (2011)
Raus Balind, S., Manojlović-Stojanovski, M., Milošević, V., Todorović, D., Nikolić, L.J., Petković, B.: Short and long term exposure to alternating magnetic field (50 Hz, 0.5 mT) affects rat pituitary ACTH cells: stereologgical study: (2016). Environ. Toxicol. 31, 461–468
Al-Akhras, M.A., Darmani, H., Elbetieha, A.: Influence of 50 Hz magnetic fields on sex hormones and other fertility parameters of adult male rats. Bioelectromagnetics 27, 127–131 (2006)
Ahmet, K., Gokhan, C., Fehmi, O., Mehmet, A., Hakan, M., Sukru, O.: Effects of 900 MHz electromagnetic field on THS and thyroid hormones in rats. Toxicol. Lett. 157(3), 257–262 (2005)
Tang, J., Zhang, Y., Yang, L., Chen, Q., Tan, L., Zuo, S., Feng, H., Chen, Z., Zhu, G.: Exposure to 900 MHz electromagnetic fields activates the mkp-1/ERK pathway and causes blood-brain barrier damage and cognitive impairment in rats. Brain Res. 1601, 92–101 (2015)
Attia, A.A., Yehia, A.M.: Histological, ultrastructural and immunohistochemical studies of the low frequency electromagnetic field effect on thymus, spleen and liver of albino swiss mice. Pak. J. Biol. Sci. 5, 931–937 (2002)
Blank, M., Goodman, R.: Low frequency electromagnetic fields evoke the stress response: mechanism of interaction with DNA and biomedical applications”. IEEE Trans. Plasma Sci. 30, 1497–1500 (2002)
Sener, D.R., Davis, G.E.: Angiogenesis. Cold Spring Harb. Perspect. Biol. 200 3:8:a005090
Tepper, O.M., Callaghan, M.J., Chang, E.I., Galiano, R.D., Bhatt, K.A., Baharestani, S., Gan, J., Simon, B.: Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF-2. FASEB J. (2004)
Yen-Patton, G.P.A., Patton, W.F., Beer, D.M., Jacobson, B.: Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro. J. Cell. Physiol. (1999)
Kališnik, M.: Stereological Section. ZDAJ, IHE, Faculty of Medicine, Ljubljana (1985)
Alexandrescu, S., Tatevian, N., Olutoye, O., Brown, E.R.: Persistent hyperinsulinemic hypoglycemia of infancy: constitutive activation of the mTOR pathway with associated exocrine-islet transdifferentiation and therapeutic implications. Int. Clin. Exp. Pathol. 3(7), 691–705 (2010)
Teta, M., Long, S.Y., Wartschow, L.M., Rankin, M.M., Kushner, J.A.: Very slow turnover of beta-cells in aged adult mice. Diabetes 54(9), 2557–2567 (2005)
Tzaphlidou, M., Fotiou, E.: Collagen as a target for electromagnetic fields. Effects of 910-MHz on rat brain. Bioelectromagnetics 183:183–93 (2006)
Böttinger, E.R., Jakubczak, J.L., Roberts, I.S., Mumy, M., Hemmati, P., Bagnall, K., Merlino, G., Wakefileld, L.M.: Expression of a dominant-negative mutant TGF-beta type II receptor in transgenic mice reveals essential roles for TGF-beta in regulation of growth and differentiation in the exocrine pancreas. EMBO J. 16(10), 2621–2633 (1997)
McKay, J.C., Frank, S., Prato, S.F., Thomas, W.A.: A Literature Review: The effects of magnetic field exposure on blood ow and blood vessels in the microvasculature. Bioelectromagnetics 28(2), 81–98 (2007)
Okano, H., Onmori, R., Tomita, N., Ikada, Y.: Effects of a moderate-intensity static magnetic field on VEGF-A stimulated endothelial capillary tubule formation in vitro. Bioelectromagnetics 27, 628–640 (2006)
Dai, C., Brissova, M., Reinert, R.B., Nyman, L., Liu, E.H., Thompson, C., Shostak, A., Shiota, M., Takahashi, C., Powers, A.C.: Pancreatic islet vasculature adapts to insulin resistance through dilation and not angiogenesis. Diabetes 62(12), 4144–53 (2013)
Delle Monache, S., Alessandro, R., Iorio, R., Gualtieri, G., Colonna, R.: Extremely low frequency electromagnetic fields (ELF-EMFs) induce in vitro angiogenesis process in human. Bioelectromagnetics 29(8), 640–648 (2008)
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Paraš, S., Vojinović, N., Amidžić, L. (2020). Impact of High Frequency Electromagnetic Fields on Process of Angiogenesis. In: Badnjevic, A., Škrbić, R., Gurbeta Pokvić, L. (eds) CMBEBIH 2019. CMBEBIH 2019. IFMBE Proceedings, vol 73. Springer, Cham. https://doi.org/10.1007/978-3-030-17971-7_6
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DOI: https://doi.org/10.1007/978-3-030-17971-7_6
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