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Miniaturized Stimulator for Imaging of Live Cell Responses to High Frequency Mechanical Vibration

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CMBEBIH 2019 (CMBEBIH 2019)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 73))

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Abstract

Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, Gpeak ≥ 1 Gpeak) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, Gpeak < 1 Gpeak) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 Gpeak, 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications.

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Acknowledgements

This study was funded by Finnish Funding agency for Innovation (TEKES, Human spare parts project), City of Tampere, Instrumentariumin tiedesäätiö s.r. foundation, Finnish Cultural Foundation (The Kainuu Regional Fund), and the Finnish Academy of Science and Letters (Väisälä Foundation).

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Faculty of Medicine and Health Technology, Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland

    Heidi T. Halonen, Jari A. K. Hyttinen & Teemu O. Ihalainen

Authors
  1. Heidi T. Halonen
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  2. Jari A. K. Hyttinen
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  3. Teemu O. Ihalainen
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Corresponding author

Correspondence to Heidi T. Halonen .

Editor information

Editors and Affiliations

  1. International Burch University, Sarajevo, Bosnia and Herzegovina

    Almir Badnjevic

  2. Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and Herzegovina

    Ranko Škrbić

  3. International Burch University, Sarajevo, Bosnia and Herzegovina

    Lejla Gurbeta Pokvić

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Halonen, H.T., Hyttinen, J.A.K., Ihalainen, T.O. (2020). Miniaturized Stimulator for Imaging of Live Cell Responses to High Frequency Mechanical Vibration. In: Badnjevic, A., Škrbić, R., Gurbeta Pokvić, L. (eds) CMBEBIH 2019. CMBEBIH 2019. IFMBE Proceedings, vol 73. Springer, Cham. https://doi.org/10.1007/978-3-030-17971-7_4

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  • DOI: https://doi.org/10.1007/978-3-030-17971-7_4

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-17970-0

  • Online ISBN: 978-3-030-17971-7

  • eBook Packages: EngineeringEngineering (R0)

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