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Mushrooms: Isolation and Purification of Exopolysaccharides

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Biology of Macrofungi

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Mushrooms are kind of fungi that have been used as food and medicine, with rich sources of bioactive natural components. Polysaccharide is one of main bioactive constituents in mushroom, mostly composed of glucose by α/β glycosidic bonds. Because of its good benefit for health, mushroom polysaccharide can be used as functional food ingredients. When the polysaccharides are extracted from mushroom, different solvents, such as aqueous solution, alkaline solution and acidic solution are commonly used for pretreatment. The application of new science and technology, such as pressurized liquid extraction (PLE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical fluid extraction (SFE), are employed to improve the polysaccharides extraction rate. Further purification with some techniques, involving freeze–thawing, treatment with Fehling solution, fractional precipitation, ethanol precipitation, closed dialysis and ultrafiltration as well as column fractionation are also discussed detailly in this chapter.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi Province, China

    Yuxiao Wang, Xiaojun Huang & Shaoping Nie

Authors
  1. Yuxiao Wang
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  2. Xiaojun Huang
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  3. Shaoping Nie
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Corresponding author

Correspondence to Shaoping Nie .

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Editors and Affiliations

  1. Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Bhim Pratap Singh

  2. Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Lallawmsanga

  3. Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Ajit Kumar Passari

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Wang, Y., Huang, X., Nie, S. (2018). Mushrooms: Isolation and Purification of Exopolysaccharides. In: Singh, B., Lallawmsanga, Passari, A. (eds) Biology of Macrofungi. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-02622-6_9

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