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Mycoplasma and Ureaplasma

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Molecular Typing in Bacterial Infections

Part of the book series: Infectious Disease ((ID))

Abstract

Several species of mycoplasmas and ureaplasmas are now well-known human pathogens responsible for a broad array of conditions involving the respiratory and urogenital tracts of adults and infants and they may cause severe systemic disease in susceptible hosts. Greater attention is now being given to these organisms in diagnostic microbiology largely as a result of improved methods for their laboratory detection made possible by powerful molecular-based techniques. PCR-based technology can be employed for primary detection in clinical specimens as well as to characterize the organisms genetically for epidemiological purposes. For slow-growing species such as Mycoplasma pneumoniae and Mycoplasma genitalium, molecular-based detection is the only practical means for microbiological diagnosis. Most molecular-based methods used for detection and characterization of conventional bacteria have now been applied to these organisms and some methods seem to work better than others. A complete genome sequence is now available for at least one strain of all of the important human pathogens in the Mycoplasma and Ureaplasma genera. Information gained from genome analyses and improvements in efficiency of DNA sequencing are expected to advance the field of molecular detection and genotyping significantly over the next few years. This chapter provides a summary and critical review of methods suitable for detection and characterization of mycoplasmas and ureaplasmas of humans with emphasis on molecular genotypic techniques.

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Authors and Affiliations

  1. University of Alabama at Birmingham, Birmingham, AL, USA

    Ken B. Waites & Li Xiao

  2. Department of Pathology, University of Alabama at Birmingham, WP 230, 619 19th Street South, Birmingham, AL, 35226, USA

    Ken B. Waites

  3. J. Craig Venter Institute, Rockville, MD, USA

    Vanya Paralanov & John I. Glass

  4. University of Maryland, Baltimore, MD, USA

    Rose M. Viscardi

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  2. Li Xiao
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  5. John I. Glass
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Correspondence to Ken B. Waites .

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  1. Instituto Nacional de Controle de, Qualidade em Saúde, Fundação Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Rio de Janeiro, 21045-900, Rio de Janeiro, Brazil

    Ivano de Filippis

  2. Microbiology, BioReliance Corporation, Broschart Road 14920, Rockville, 20850, Maryland, USA

    Marian L. McKee

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Waites, K.B., Xiao, L., Paralanov, V., Viscardi, R.M., Glass, J.I. (2013). Mycoplasma and Ureaplasma. In: de Filippis, I., McKee, M. (eds) Molecular Typing in Bacterial Infections. Infectious Disease. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-185-1_16

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