Abstract
The realization of the potential in research and therapy of human pluripotent stem cells (hPSCs), both embryonic and induced, will require mass production of undifferentiated and differentiated cells. Clearly, the conventional two-dimensional techniques for hPSCs are inadequate for such. This chapter focuses on suspension culturing of undifferentiated hPSCs, a method suitable for the robust production of these cells in controlled bioreactors.
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References
Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, Thomson JA (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227:271–278
Amit M, Margulets V, Segev H, Shariki C, Laevsky I, Coleman R, Itskovitz-Eldor J (2003) Human feeder layers for human embryonic stem cells. Biol Reprod 68:2150–2156
Amit M, Shariki K, Margulets V, Itskovitz-Eldor J (2004) Feeder and serum-free culture system for human embryonic stem cells. Biol Reprod 70:837–845
Amit M, Chebath J, Marguletz V, Laevsky I, Miropolsky Y, Shariki K, Peri M, Revel M, Itskovitz-Eldor J (2010) Suspension culture of undifferentiated human embryonic and induced pluripotent stem cells. Stem Cell Rev Rep 6:248–259
Amit M, Laevsky I, Miropolsky Y, Shariki K, Peri M, Itskovitz-Eldor J (2011) Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nat Protoc 6:572–579
Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R (1985) The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87:27–45
Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, Soreq H, Benvenisty N (2000) Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. Mol Med 6:88–95
Ludwig TE, Levenstein ME, Jones JM, Berggren WT, Mitchen ER, Frane JL, Crandall LJ, Daigh CA, Conard KR, Piekarczyk MS, Llanas RA, Thomson JA (2006) Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24:185–187
Richards M, Fong CY, Chan WK, Wong PC, Bongso A (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20:933–936
Oh SK, Chen AK, Mok Y, Chen X, Lim UM, Chin A, Choo AB, Reuveny S (2009) Long-term microcarrier suspension cultures of human embryonic stem cells. Stem Cells Res 2:219–230
Olmer R, et al. (2010) Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium. Stem Cell Res 5:51–64
Phillips BW, Horne R, Lay TS, Rust WL, Teck TT, Crook JM (2008) Attachment and growth of human embryonic stem cells on microcarriers. J Biotechnol 138:24–32
Singh H, Mok P, Balakrishnan T, Rahmat SN, Zweigerdt R (2010) Up-scaling single cell-inoculated suspension culture of human embryonic stem cells. Stem Cell Res 4:165–179
Steiner D, et al. (2010) Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotech 28:361–364
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920
Zandstra PW, Nagy A (2001) Stem cell bioengineering. Annu Rev Biomed Eng 3:275–305; review
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Amit, M., Itskovitz-Eldor, J. (2012). Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures. In: Amit, M., Itskovitz-Eldor, J. (eds) Atlas of Human Pluripotent Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-548-0_4
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DOI: https://doi.org/10.1007/978-1-61779-548-0_4
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