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The Effect of Temperature, Pressure, Exposure Time, and Depressurization Rate on Lipase Activity in SCCO2

  • Conference paper
Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO

Part of the book series: Biotechnology for Fuels and Chemicals ((ABAB))

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Abstract

We investigated the influence of temperature, pressure, exposure time, and decompression rate on lipase activity in high-pressure CO2medium. A high-pressure, variable-volume view cell was employed in the experiments, varying the temperature from 30 to 70°C in the pressure range of 70–250 bar at various high-pressure exposure times (60–360 min) and adopting several decompression rates (10–200 kg/[m3·min]). The results obtained show that an increase in temperature and density led to an enhancement of enzyme activity losses while the decompression rates had a weak influence on enzyme inactivation.

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Author information

Author notes

  1. Débora de Oliveira

    Present address: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621-Erechim-RS, 99700-000, Brazil

Authors and Affiliations

  1. Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621-Erechim-RS, 99700-000, Brazil

    Marcelo Lanza, Wagner Luís Priamo, José Vladimir Oliveira & Cláudio Dariva

Authors
  1. Marcelo Lanza
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  2. Wagner Luís Priamo
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  3. José Vladimir Oliveira
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  4. Cláudio Dariva
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  5. Débora de Oliveira
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Corresponding author

Correspondence to Débora de Oliveira .

Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara Evans  & 

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© 2004 Springer Science+Business Media New York

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Lanza, M., Priamo, W.L., Oliveira, J.V., Dariva, C., de Oliveira, D. (2004). The Effect of Temperature, Pressure, Exposure Time, and Depressurization Rate on Lipase Activity in SCCO2 . In: Finkelstein, M., McMillan, J.D., Davison, B.H., Evans, B. (eds) Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO. Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-837-3_16

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  • DOI: https://doi.org/10.1007/978-1-59259-837-3_16

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9873-8

  • Online ISBN: 978-1-59259-837-3

  • eBook Packages: Springer Book Archive

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