Skip to main content
SpringerLink
Log in

Effect of Temperature, Moisture, and Carbon Supplementation on Lipase Production by Solid-State Fermentation of Soy Cake by Penicillium simplicissimum

  • Conference paper
Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO

Part of the book series: Biotechnology for Fuels and Chemicals ((ABAB))

Abstract

The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Jung, F., Cammarota, M. C., and Freire, D. M. G. (2002), Biotechnol. Lett. 24, 1797–1802.

    Article  CAS  Google Scholar 

  2. Cammarota, M. C., Teixeira, G. A., and Freire, D. M. G. (2001), Biotechnol. Lett. 23, 1591–1595.

    Article  CAS  Google Scholar 

  3. Castilho, L. R, Polato, C. M. S., Baruque, E. A., and Sant’Anna, G. L., Jr. (2000), Biochem. Eng. J. 4, 239–247.

    Article  CAS  Google Scholar 

  4. Del Bianchi, V. L., Moraes, I. O., and Capalbo, D. M. F. (2001), in Biotecnologia Industrial: Engenharia Bioquímica, vol. 3, Schmidell, W., Lima, U.A., Aquarone, E., and Borzani, W., eds., Edgard Blücher, São Paulo, Brazil.

    Google Scholar 

  5. Freire, D. M. G. (1996), PhD thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.

    Google Scholar 

  6. Freire, D. M. G., Gomes, P. M., Bon, E. P. S., and Sant’Anna, G. L., Jr. (1997), Revista Microbiologia 28(1), 6–12.

    Google Scholar 

  7. Leal, M. C. M. R., (2000), MS thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.

    Google Scholar 

  8. Pregnolatto, W., Pregnolatto, N. P. (coord.), and Rebocho, D. D. E. (ed.) (1985), Normas analíticas do Instituto Adolfo Lutz: vol.1. Métodos químicos efísicos para análise de alimentos, 3rd Ed., Instituto Adolfo Lutz, São Paulo, Brazil.

    Google Scholar 

  9. MCT, Ministério da Ciência e Tecnologia (Ministry of Science and Technology) (1985), Technical Report on Vegetable Oils, MCT.

    Google Scholar 

  10. Gombert, A. K., Pinto, A. L., Castilho, L. R., and Freire, D. M. G. (1999), Process Biochem. 35, 85–90.

    Article  CAS  Google Scholar 

  11. Palma, M. B., Pinto, A. L., Gombert, A. K., Seitz, K. H., Kivatinitz, S. C., Castilho, L. R., and Freire, D. M. G. (2000), Appl. Biochem. Biotechnol. 84–86, 1137–1145.

    Article  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Marco Di Luccio

    Present address: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, RS, Brazil

Authors and Affiliations

  1. Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, RS, Brazil

    Fernando Capra, Najara P. Ribeiro, Gean D. L. P. Vargas & Débora de Oliveira

  2. Department of Biochemistry-IQ/UFRJ, Centro de Tecnologia, Bl. A, Sala 549-2, Rio de Janeiro, 21945-900, RJ, Brazil

    Denise M. G. Freire

Authors
  1. Marco Di Luccio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fernando Capra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Najara P. Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gean D. L. P. Vargas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Denise M. G. Freire
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Débora de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marco Di Luccio .

Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara Evans  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Springer Science+Business Media New York

About this paper

Cite this paper

Luccio, M.D., Capra, F., Ribeiro, N.P., Vargas, G.D.L.P., Freire, D.M.G., de Oliveira, D. (2004). Effect of Temperature, Moisture, and Carbon Supplementation on Lipase Production by Solid-State Fermentation of Soy Cake by Penicillium simplicissimum . In: Finkelstein, M., McMillan, J.D., Davison, B.H., Evans, B. (eds) Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO. Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-837-3_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-59259-837-3_15

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9873-8

  • Online ISBN: 978-1-59259-837-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation