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Isolation and Detection of Pathogenic Escherichia coli in Foods

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Detection and Typing Strategies for Pathogenic Escherichia coli

Part of the book series: SpringerBriefs in Food, Health, and Nutrition ((BRIEFSFOOD))

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Abstract

There is currently no single method that can be used to enrich, isolate, or select for the various E. coli pathotypes that exist. Isolation and detection methods for foodborne E. coli has focused on the Shiga-toxin producing Escherichia coli (STEC) group, particularly for the serogroup O157. However, this is expanding to include non-O157 serogroups as their role in foodborne disease is further elucidated. Traditional methods of detecting pathogenic E. coli are often time-consuming and labor-intensive as they require the growth of the pathogen in culture media, followed by isolation and biochemical and/or serological identification or even further confirmation steps. Various methods have been developed to rapidly identify pathogens including phenotypic assays, such as antibody and modifications of conventional tests, and molecular detection assays. Molecular detection methods can be used to rapidly screen food samples for E. coli pathotypes. However, popular methods such as Polymerase Chain Reaction (PCR) have limitations, including the inability to differentiate live cells from dead and the inhibition of PCR by complex media. One major limitation of all detection methods is the lack of sensitivity to directly detect the small number of pathogenic cells in a food sample. However, further developments and the use of a combination of methods may facilitate the accurate detection and possible enumeration of these pathogens in the future.

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Authors and Affiliations

  1. Institute of Environmental Science and Research, Riccarton, Christchurch, New Zealand

    Lucia Rivas

  2. CSIRO Food and Nutrition Flagship, Archerfield, Queensland, Australia

    Glen E. Mellor

  3. CSIRO Food and Nutrition Flagship, Werribee VIC, Victoria, Australia

    Kari Gobius & Narelle Fegan

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  1. Lucia Rivas
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  2. Glen E. Mellor
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  3. Kari Gobius
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  4. Narelle Fegan
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Correspondence to Lucia Rivas .

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Rivas, L., Mellor, G., Gobius, K., Fegan, N. (2015). Isolation and Detection of Pathogenic Escherichia coli in Foods. In: Detection and Typing Strategies for Pathogenic Escherichia coli. SpringerBriefs in Food, Health, and Nutrition. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2346-5_2

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