Abstract
Transport across the basolateral and canalicular domains of the hepatocyte plasma membrane represents two important but very distinct processes essential for the movement of drugs or endogenous compounds from the plasma into the hepatocyte and from the hepatocyte into bile. This chapter will cover transport in isolated rat hepatocytes as a method that is very useful and practical for characterizing transport across the basolateral domain. The isolated perfused rat liver has also been used extensively to characterize transport from plasma to liver; this method is covered in Chapter 10. Cultured hepatocytes have also been used to study the mechanisms of transport of substrates into the hepatocyte; methods for preparation of cultured hepatocytes are covered in Chapter 9.
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© 1996 Springer Science+Business Media New York
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Vore, M., Liu, Y., Ganguly, T.C. (1996). Isolated Hepatocytes. In: Borchardt, R.T., Smith, P.L., Wilson, G. (eds) Models for Assessing Drug Absorption and Metabolism. Pharmaceutical Biotechnology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1863-5_8
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DOI: https://doi.org/10.1007/978-1-4899-1863-5_8
Publisher Name: Springer, Boston, MA
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