Abstract
Physical and chemical mutagens induce frank breaks in DNA which reduce its single-strand molecular weight. Other nonbreak lesions in the DNA can often be converted into strand breaks by chemical and enzymatic means. Using agarose gel electrophoresis along with various cleavage schemes, the average density of breaks and various lesion classes along mammalian DNA can be determined.
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Drouin, R., Gao, S., Holmquist, G.P. (1996). Agarose Gel Electrophoresis for DNA Damage Analysis. In: Pfeifer, G.P. (eds) Technologies for Detection of DNA Damage and Mutations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0301-3_3
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DOI: https://doi.org/10.1007/978-1-4899-0301-3_3
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