Abstract
Humans are constantly exposed to xenobiotics present in the environment, the food, or from clinical use. To counteract the toxic accumulation of foreign molecules, living organisms have evolved a defense system comprising a family of detoxifying enzymes (cytochromes P450, CYPs). Numerous xenobiotics induce CYP genes: the induction is an essential adaptive mechanism that allows organisms to adjust their detoxification capacity according to the needs. Only in a few cases are the induction mechanisms understood to some extent. While it is well admitted that both transcriptional and post-transcriptional (mRNA processing and turnover) control is important for cyp genes expression, very little is known about the post-transcriptional mechanisms Of regulation. We have investigated the post-transcriptional regulation of two CYP genes, CYP1A2 and CYP2A5, and identified RNA-binding proteins interacting with their mRNA in an inducer-dependent manner. The strategy used to characterize the RNA protein interaction and to identify the relevant RNA sequences as well as the regulatory factors are described in this chapter.
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Lang, M.A., Raffalli-Mathieu, F. (2002). Cytochrome P450 RNA—Protein Interactions. In: Sandberg, K., Mulroney, S.E. (eds) RNA Binding Proteins. Endocrine Updates, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6446-8_13
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DOI: https://doi.org/10.1007/978-1-4757-6446-8_13
Publisher Name: Springer, Boston, MA
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