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Immobilized Polynucleotides and Nucleic Acids

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Immobilized Biochemicals and Affinity Chromatography

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 42))

Abstract

The use of immobilized nucleic acids and polynucleotides in the study of nucleic acids and their associated enzymes has become widespread in recent years. There are two main areas of application: the fractionation of nucleic acids and polynucleotides through base-paired complex formation and the isolation and purification of nucleic acid-associated enzymes by affinity chromatography. There are now a number of methods for the preparation of immobilized polynucleotides and the potential uses of these materials depend to some extent on the means by which the polymers are attached to the insoluble supports.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907, USA

    P. T. Gilham

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  1. P. T. Gilham
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Editor information

Editors and Affiliations

  1. Department of Chemistry, University of South Carolina, Columbia, South Carolina, USA

    R. Bruce Dunlap

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© 1974 Plenum Press, New York

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Gilham, P.T. (1974). Immobilized Polynucleotides and Nucleic Acids. In: Dunlap, R.B. (eds) Immobilized Biochemicals and Affinity Chromatography. Advances in Experimental Medicine and Biology, vol 42. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6982-0_14

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  • DOI: https://doi.org/10.1007/978-1-4684-6982-0_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-6984-4

  • Online ISBN: 978-1-4684-6982-0

  • eBook Packages: Springer Book Archive

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