Abstract
The removal of introns from the primary transcript occurs by RNA splicing. Three major types of RNAs; tRNA, rRNA and mRNA, are known to contain introns. There are two general questions concerning any splicing mechanism: first, how is the precise recognition and alignment of the splice junctions achieved in introns whose lengths vary from a few hundred nucleotides to several thousands of nucleotides, and second, what are the biochemical mechanisms of the cleavage and ligation reactions.
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References
Brody, E., and Abelson, J., 1985, The “Spliceosome”: Yeast pre-messenger RNA associates with a 40S complex in a splicing dependent reaction, Science 228:963–967.
Cech, T. R., and Bass, B. L., 1986, Biological catalysis by RNA, Ann. Rev. Biochem., 55:599–629.
Chang, T.-H., Clark, M. W., Lustig, A. J., Cusick, M. E., and Abelson, J., 1988, RNA11 protein is associated with yeast spliceosome and is localised in the periphery of the cell nucleus, Mol. Cell Biol., 8:2379–2393.
Cheng, S.-C., and Abelson, J., 1987, Spliceosome assembly in yeast. Genes Dev., 1:1014–1027.
Couto, J. R., Tamm, J. J., Parker, R., and Guthrie, C., 1987, A trans acting supressor restores splicing of a yeast intron with a branch point mutation, Genes Dev., 1:445–455.
Frendewey, D., and Keller, W., 1985, Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences, Cell, 42:355–367.
Grabowski, P. J., Seiler, S. R., and Sharp, P. A., 1985, A multicomponent complex is involved in splicing of messenger RNA precursors, Cell, 42:345–353.
Hartwell, L. H., 1967, Macromolecular synthesis in temperature-sensitive mutants in yeast, J. Bact., 93:1662–1670.
Jackson, S. P., Lossky, M., and Beggs, J. D., 1988, Cloning of RNA8 gene of Saccharomyces cerevisiae. Detection of the RNA8 protein and demonstration that it is essential for nuclear pre-mRNA splicing, Mol. Cell Biol., 8:1067–1075.
Last, R. L., and Woolford, J. L., 1986, Identification and nuclear localization of yeast pre-mRNA processing components: RNA2 and RNA3 proteins, J. Cell Biol., 103:2103–2112.
Last, R. L., Stavenhagen, J. B., and Woolford, J. L., 1984, Isolation and characterization of the RNA2, RNA3 and RNA11 genes of Saccharomyces cerevisiae, Mol. Cell Biol., 4:2396–2405.
Last, R. L., Maddock, J. R., Woolford, J. L., 1987, Evidence for related functions of the RNA genes of Saccharomyces cerevisiae, Genetics, 117:619–631.
Lee, M. G., Young, R. A., and Beggs, J. D., 1984, Cloning of the RNA2 gene of Saccharomyces cerevisiae, EMBO J., 3:2825–2830.
Lin, R.-J., Lustig, A. J., and Abelson, J., 1987, Splicing of yeast nuclear pre-mRNA in vitro requires functional 40S spliceosomes and several extrinsic factors, Genes Dev., 1:7–18.
Lossky, M., Anderson, G. J., Jackson, S. P., and Beggs, J. D., 1987, Identification of yeast snRNP protein and the detection of snRNP-snRNP interactions, Cell, 51:1019–1026.
Lustig, A. J., Lin, R.-J., and Abelson, J., 1986, The yeast RNA gene products are essential for mRNA splicing in vitro, Cell, 47:953–963.
Maniatis, T., and Reed, R., 1987, The role of small nuclear ribonucleoproteins in pre-mRNA splicing, Nature, 325:673–678.
Pearson, N. J., Thornburn, D. C., and Haber, J. E., 1982, A supressor of temperature sensitive RNA mutations that affect general and specific messenger RNA processing in yeast, Mol. Cell Biol., w:571–577.
Rosbash, M., Harris, P. K. W., Woolford, J. L., and Teem, J. L., 1981, The effect of temperature sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast, Cell, 24:679–686.
Shapiro, M. B., Senapathy, P., 1987, RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression, Nucl. Acids Res., 15:7155–7174.
Slotyk, A., Tropak, M., and Friesen, J. D., 1984, Isolation and characterization of the RNA2, RNA4 and RNA11 genes of Saccharomyces cerevisiae, J. Bact., 160:1093–1100.
Teem, J. L., Rodrigues, J. R., Tung, L., and Rosbash, M., 1983, The RNA2 mutation affects the processing of actin mRNA as well as ribosomal protein mRNAs, Mol. Gen. Genet., 192:101–103.
Warner, J. R., 1987, Applying genetics to the splicing problem, Genes Dev., 1:1–3.
Warner, J. R., and Udem, S. A., 1972, Temperature sensitive mutations affecting ribosome synthesis in Saccharomyces cerevisiae, J. Mol. Biol., 65:243–257.
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© 1989 Plenum Press, New York
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Vijayraghavan, U., Abelson, J. (1989). Yeast pre-mRNA Splicing Mutants. In: Grunberg-Manago, M., Clark, B.F.C., Zachau, H.G. (eds) Evolutionary Tinkering in Gene Expression. NATO ASI Series, vol 169. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5664-6_18
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DOI: https://doi.org/10.1007/978-1-4684-5664-6_18
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