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Novel Insulin-Related Sequences in Fetal Brain

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Gene Expression and Cell-Cell Interactions in the Developing Nervous System

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 181))

Abstract

An examination of the literature leaves no doubt that there is a moiety which cross-reacts with anti-insulin antibodies in the central and peripheral nervous systems. However, there is considerable doubt as to how much of this material there is, where exactly it resides, where it originates and what role it plays. In this chapter, we will review the evidence for the presence and synthesis of insulin or insulin-like peptides in the nervous system and we will describe how we are using recombinant DNA technology to identify and characterize RNA sequences homologous, but not identical, to sequences encoding insulin. (We will not deal with immunoreactive insulin in invertebrates [for review, see Le Roith et al].)

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References

  • Ando T., 1966, A nuclease specific for heat-denatured DNA is isolated from a product of Aspergillus oryzae. Biochem. Biophys. Acta 114:158–68

    Article  PubMed  CAS  Google Scholar 

  • Aviv H. and Leder P., 1972, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose, Proc. Natl, Acad. Sci. USA 69: 1408–1412.

    Article  CAS  Google Scholar 

  • Baltimore D., 1970, RNA-dependent una polymerase in virions of RNA tumor viruses. Nature 226:1209–1211.

    Article  PubMed  CAS  Google Scholar 

  • Baskin D.G., Porte u., Guest K. and Dorsa D.M., 1983, Regional concentrations of insulin in the rat brain, Endocrinology 112: 898–903.

    Article  PubMed  CAS  Google Scholar 

  • Bell G. I., Swain W.F., Pictet R., Cordel 1 B., Goodman H.M. and Rutter W.J., 1979, Nucleotide sequence of a cDNA clone encoding human preproinsulin. Nature 282:525–527.

    Article  PubMed  CAS  Google Scholar 

  • Berggren V., Engel J. and uijequist S., 1983, Differential effects of insulin on brain monoamine metabolism in rats, Acta Phrmacol. et toxicol 53: 39–43.

    Article  CAS  Google Scholar 

  • Birch N.P., Christie D.L. and Renwick A.G.C., 1984, Immunoreactive insulin from mouse brain cells in culture and whole rat brain, Biochem. J. 217: in press.

    Google Scholar 

  • Binoux M., Hossenlopp P., Lassarre C. and Hardouin N., 1981, Production of insulin-like growth factors and their carrier by rat pituitary gland and brain expiants in culture, FEBS Letts. 24: 178–184.

    Article  Google Scholar 

  • Blattner F.R., Williams B.G., Blechl A.E., Thompson K.D., Faber H.E., Furlong L.A., Grunwald D.J., Kiefer D.O., Moore D.D., Schumm J.W., Sheldon E.L., and Smithies O., 1977, Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196: 161–169.

    Article  PubMed  CAS  Google Scholar 

  • Bolivar F., Rodriguez R.L., Greene P.J., Betlach M.C., Heyneker H.L., Boyer H.H., Crossa J.H. and Falkow S., 1977, Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 1:95–113.

    Article  Google Scholar 

  • Bollum R., 1974, Terminal deoxynucleotide transferase, The Enzymes 10: 195.

    Article  Google Scholar 

  • Bothwell M., 1982, Insulin and somatomedin MSA promote nerve growth factor in dependent neurite formation by cultured chick dorsal root ganglionic sensory neurons, J. Neurosci. Res. 8: 225–231.

    Article  PubMed  CAS  Google Scholar 

  • Bottenstein J.E., Skaper S.D., Varon S.S., Sato G.H., 1980, Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium. Exp. Cell Res. 125: 183–190.

    Article  PubMed  CAS  Google Scholar 

  • Britten R.J., Graham D.E. and Neufeld B.R., 1974, Analysis of repeating DNA sequences by reassociation, in Methods in Enzymology ed. L. Grossman and K. Moldare, Academic, NY. pp. 363–418.

    Google Scholar 

  • Chaudhari N. and Hahn W.E., 1983, Genetic expression in the developing brain, Science 220: 924–928

    Article  PubMed  CAS  Google Scholar 

  • Chikaraishi D.M., 1979, Complexity of cytoplasmic polyadenylated and nonpolyadenylated rat brain ribonucleic acids, Biochemistry 18: 3249–3256.

    Article  PubMed  CAS  Google Scholar 

  • Chirgwin ü.M., Przybyla A.E. and Rutter W.J., 1979, Isolation of RNA in the presence of ribonuclease with quanidinium thiocyanate, Biochemistry 18: 5294–5298.

    Article  PubMed  CAS  Google Scholar 

  • Clarke L. and Carbon J., 1976, A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome, tell 9:91–99

    CAS  Google Scholar 

  • Colca J.R. aricThazelwood R.L., 1982, Persistence of immunoreactive insulin, glucagon and pancreatic polypeptide in the plasma of depancreatized chickens, J. Endocrin. 92: 317–326.

    Article  CAS  Google Scholar 

  • Cold Spring Harbor Symposia on Quantitative Biology, 1983, Molecular Neurobiology vol. 48, part 1 and 2.

    Google Scholar 

  • DeMontis M. G. Olianas M.C., Haber B. and Tagliamonte A., 1978, Increase in large neutral amino acid transport into brain by insulin. J. Neurochem. 30: 121–124.

    Article  CAS  Google Scholar 

  • dePablo F., Roth J., Hernandez E. and Pruss R.M., 1982, Insulin is present in chicken eggs and early chick embryos, Embryology 111: 1909–1916.

    CAS  Google Scholar 

  • Dorn A., Bernstein H-G. Hahn H-J. Ziegler M. and Rummelfánger H., 1981, Insulin immunoreactivity of rodent CNS: apparent species differences but good correlation with raioimmunological data, Hi stochern. 71: 609–616.

    CAS  Google Scholar 

  • Dorn A., Bernstein H-G. Kostmann G., Hahn H-J. and Ziegler M., 1980, An immunofluorescent reaction appears to insulin antiserum in different CMS regions of two rat species, Acta Histochern, 66: 276–278.

    Article  CAS  Google Scholar 

  • Dorn A., Bernstein H-G. Rinne A. Hahn H-J and Ziegler M., 1982, Insulin-like immunoreactivity in human brain: a preliminary report, Hi stochern. 74: 293–300.

    CAS  Google Scholar 

  • Dugaiczyk A., Hedgpeth J., Boyer H.W. and Goodman H.M., 1974, Physical identity of the SY40 deoxyribonucleic acid sequence recognized by the Eco R1 restriction endonuclease and modification methyl ase. Biochemistry 13:503–512.

    Article  PubMed  CAS  Google Scholar 

  • Efstratiadis A. and Villa-Komaroff L., 1979, Cloning of double-stranded cDNA in Genetic engineering: Principles andmethods vol 1, edited by Hollaender, A., and Setlow, J. Hew York Plenum pp 15–36.

    Google Scholar 

  • Eng J. and Yallow R.S., 1980, Insulin recoverable from tissues, Diabetes 29: 105–109.

    Article  PubMed  CAS  Google Scholar 

  • Eng J. and Yallow R.S., 1981, Evidence against extrapancreatic insulin synthesis, Proc. Natl. Acad. Sci. USA 78: 4576–4578.

    Article  PubMed  CAS  Google Scholar 

  • Enquist L. and Sternberg N., 1980, In vitro packaging of DAM vectors and their use in cloning DNA fragments, in Methods inEnzymology, v.68, ed. R. Wm, Academic Press, NY pp. 281–298.

    Google Scholar 

  • Frank J.L.H. and Pardridge W.M. Insulin binding to brain microvessels in Advances in Metabolic disorders 10: CNS regulation of carbohydrate metabolism” A.J. Szalo, e., Academic New York, 1983.

    Google Scholar 

  • Giddings S., Chirgwin J. and Permutt M.A., 1981, Expression of the insulin gene in pancreatic and extrapancreatic tissues, Endocrinology Suppl. A-624, 238.

    Google Scholar 

  • Gilbert W. and Yinakomaroff L., 1980, Useful proteins from recombinant bacteria. Scientific American 242–4: 74–94.

    Article  Google Scholar 

  • Goff S.P. and Berg P., 1978, Excision of DNA segments introduced into cloning vectors by the polyidat, method. Proc. Nat’l. Acac. Sci. USA 76:106–110.

    Google Scholar 

  • Goldberg M.L., Lifton R.P., Starke G.R. and Williams J.G., 1979, Isolation of specific RNAs using DNA covalently linked to diazobenzlomethyl cellulose or paper, in Methods in Enzymology 68: 206–222 ed. R. Wu, Academic Press.

    Google Scholar 

  • Gonzalez A., Wentworth B., Dobner P. and Villa-Komaroff L., RNA from nonpancreatic tissues contains sequences homologous to insulin, in preparation.

    Google Scholar 

  • Goodner C.J. and Berrie M.A., 1977, The failure of rat hypothalamic tissues to take up labeled insulin in vivo or to respond to insulin in vitro, Endocrinology 101: 605–612.

    Article  PubMed  CAS  Google Scholar 

  • Goodner C.J., Horn F. and Berrle M.A., 1980, Investigation of the effect of insulin upon regional brain glucose metabolism in the rat invivo, Endocrinology 107: 1827–1832.

    Article  PubMed  CAS  Google Scholar 

  • Green M. and Gerard G.F., 19/4, RNA-directed DNA polymerase -properties and functions in oncogenic RNA viruses and cells, in Progress in Nucleic Acid Res. Mol. Biol., ed. VI.E. Cohn, vol. 14, Academic Press, NY p. 18/-334.

    Google Scholar 

  • Greene P.J., Poonlan M.S., Nussbaum A.L., Tobias L., Garfin D.E., Boyer H.W. and Goodman H.M., 1975, Restriction and modification of a self-complementary octanucleotlde containing the Eco RI substrate J. Mol. Biol. 99:237–261.

    Article  PubMed  CAS  Google Scholar 

  • Hasel bacher G. and Humbel R., 1982, Evidence for two species of 1nsu11n-Hke growth factor II (IGF-II and “Big” IGFII) In human spinal fluid, Endocrinology 110: 1822–1824.

    Article  CAS  Google Scholar 

  • Havrankova J. and Roth J., 19/8, Insulin receptors are widely distributed in the central nervous system of the rat, Nature 272: 827–829.

    Google Scholar 

  • Havrankova J., Roth J. and Brownstein M.J., 1979, Concentrations of insulin and of insulin receptors in the brain are independent of peripheral insulin levels, J. Clin. Invest. 64: 636–642.

    Article  PubMed  CAS  Google Scholar 

  • Havrankova J., Schmechel D., Roth J. and Brownstein M., 1978, Identification of insulin in rat brain, Proc. Natl. Acad. Sci. USA 75: 5737–5741.

    Article  PubMed  CAS  Google Scholar 

  • Heidenrcich K.A., Zahniser N.R., Berhanu P., Brandenburg D. and Olefsky J.M., 1983, Structural differences between insulin receptors in the brain and peripheral target tissues, J. Biol.Chem. 258: 8527–8530.

    Google Scholar 

  • Hendriks S.A., Roth J., Rishi S. and Becker K.L., 1983, Insulin in the nervous system, in Brain Peptides ed D.T. Krieger, M.J. Brownstein and J.B. Martin, John Wiley and Sons.

    Google Scholar 

  • Hertz M.M., Paulson O.B., Barry D.I., Christiansen J.S. and Svendsen P.A., 1981, Insulin increases glucose transfer across the blood-brain barrier in man, J. Clin. Invest. 67: 597–604.

    Article  PubMed  CAS  Google Scholar 

  • Higgins N.P. and Cozzarelli N.R., 1980, DNA joining enzymes: A review, in Methods in Enzymology v.68 ed. R. Wu, Academic Press, New York, pp. 50–71.

    Google Scholar 

  • Hofstetter H., Schambock A., Van den Berg, J. and Weisman, C. 1976, Specific Excision of the Inserted DNA Segment from Hybrid Plasmids Constructed by the poly(dA) poly(dT) method. Biochem.Biophys. Acta 454: 587–591.

    Article  PubMed  CAS  Google Scholar 

  • Itakura K. and Rlggs A.D., 1980, Chemical DNA synthesis and recombinant DNA studies. Science 209:1401–1405.

    Article  PubMed  CAS  Google Scholar 

  • Jansen M., van Schalk F.M.A., Ricker A.T., Bullock B., Woods D.E., Gabbay K.H., Nussbaum A.L., Sussenback J.S. and Yan de Brande J.L., 1983, Sequence of cDNA encoding human insulin-like growth factor 1 precursor, Nature 306: 609–611.

    Article  PubMed  CAS  Google Scholar 

  • Kornberg A., 1969, Active center of DNA polymerase. Science 163: 1410–1418.

    Article  PubMed  CAS  Google Scholar 

  • Kurnit D.M., Wentworth B.M., DeLong L. and Villa-Komaroff L., 1982, The construction of cloned libraries from mRNA of human fetal tissues, Cytogen. Cell Gen. 34: 193–203.

    Article  CAS  Google Scholar 

  • Lawn R.M., Fritsch E.F., Parker R.C., Blake G., and Maniatis, 1978, The isolation and characterization of linked α-and β-globin genes from a cloned library of human DNA. Cell 15:1157–1174, 1978.

    Article  PubMed  CAS  Google Scholar 

  • Leder P., Tiemeier D. and Enquist L., 1977, EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the xgtWES, system. Science 196: 175–177.

    Article  PubMed  CAS  Google Scholar 

  • Lehrach H., Diamond D., Wozney J.N. and Boedtker, 1977, RNA molecules weight determination by gel electrophoresis under denaturing conditions, Biochemistry 16: 4743–4751.

    Article  PubMed  CAS  Google Scholar 

  • LeRoith D., Hendrick S.A., Lesniak M.A., Rishl S., Becker K.I., Havrankova J., Rosenzwieg J.L., Brownstein M.J. and Roth J., 1983, Insulin in brain and other extrapancreatic tissues of vertebrates and nonvertebrates, Advances in Metabolic Disorders 10: 303–339.

    CAS  Google Scholar 

  • Lewin B., 1983, Genes. John Wiley and Sons, Inc. NY.

    Google Scholar 

  • Lobban P.E., and Kaiser A.D., 1973, Enzymatic end-to-end joining of DNA molecules. J. Mol. Biol. 78:453–471

    Article  PubMed  CAS  Google Scholar 

  • Lomedico P.T., Saunders G.F., 1976, Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide. Nucleic Acids Res. 3:381–391.

    Article  PubMed  CAS  Google Scholar 

  • Lorenzi M., Karam J.H., Mclloroy M.B. and Forsham P., 1980, Increased growth hormone response to dopamine infusion in insulin-dependent diabetic subjects. J. Clin. Invest. 65: 146–153.

    Article  PubMed  CAS  Google Scholar 

  • Maccaleb M.L. and Meyers R.D., 1979, Striatal dopamine release is altered by glucose and insulin during push-pull perfusion of the rat caudate nucleus, Brain Res. Bull. 4: 651–656.

    Article  Google Scholar 

  • Mackenzie R.G. and Trulson M.t., 1978, Ettects of insulin and streptozotoein-induced diabetes on brain tryptophan and serotonin metabolism in rats, J. Neurochem. 30: 1205–1208.

    Article  PubMed  CAS  Google Scholar 

  • Mandel M. and Higa A., 1970, Calcium-dependent bacteriophage DNA infection. J. Mol Biol. 53:159.

    Article  PubMed  CAS  Google Scholar 

  • Maniatis T., Hardi son R.C., Lacey E., Lauer J. O’Connell C., Quon D., Gek Kee Sim and Efstratiadis A., 1978, The isolation of structural genes from libraries of eukaryotic DNA. Cell 15:687–701.

    Article  PubMed  CAS  Google Scholar 

  • Margolis R.V. and Altszuler N., 1967, Insulin in the cerebrospinal fluid, Nature 215: 1375–1376.

    Article  PubMed  CAS  Google Scholar 

  • Maxam A. and Gilbert W., 1977, A new method for sequencing DNA. Proc. Natl. Acad. Sci. USA 74:560–564

    Article  PubMed  CAS  Google Scholar 

  • Nagata S., Taira J.J., Hall A., Johnsrud L., Streuli M., Ecsodi J., Boll W., Cantell K., and Weisman C., 1980, Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature 284:316–320.

    Article  PubMed  CAS  Google Scholar 

  • Nelson T. and Brutlag D., 1980, Addition of homopolymers to the 3’-ends of duplex DNA with terminal transferase, in Methods in.Enzymology, v. 68, ed. R. Wu, Academic Press, NY, pp.41–50.

    Google Scholar 

  • Oomura r. and Kita H., 1981, Insulin acting as a modulator of feeding through the hypothalamus, Diabetologia 20: 290–298.

    Article  PubMed  CAS  Google Scholar 

  • Oomura Y., Glucose and neuronal activity, Advances in metabolic disorders 10: CNS regulation of carbohydrate metabolism, A.J. Szalo, ed., Academic, New York, 1983).

    Google Scholar 

  • Pacold S.T. and Blackard W.6., 1979, Central nervous system insulin receptors in normal and diabetic rats, Endocrinology 105: 1452–1457.

    Article  PubMed  CAS  Google Scholar 

  • Pansky B. and Hatfield J.S., 1978, Cerebral localization of insulin by immunofluorescence, Amer. J. Anat. 153: 459–467.

    Article  CAS  Google Scholar 

  • Parker K. and Yeradakls A., 1980, Stimulation of ornithine decarboxylase activity 1n neural cell culture: potential role of Insulin, J. Neurochem. 35: 155–163.

    Article  PubMed  CAS  Google Scholar 

  • Polak J.M. and Bruchan A.M.J., 1980, Motil in-cellular origin 1n human gut, J. Histochern., Cytochem. 28: 617 (abs).

    Google Scholar 

  • Raizada M.K., 1983, Localization of Insulin-like immunoreactivity In the neurons from primary cultures of rat brain, Exp. Cell Res. 14: 351–357.

    Article  Google Scholar 

  • Raizida M.K., Yang j.w. and Fellows R.E., 1980, Binding of [125I] insulin to specific receptors and stimulation of nucleotide incorporation in cells cultured from rat brain, Brain Res. 200: 389–400.

    Article  Google Scholar 

  • Ratzmann K.P. and Hampel R., 1980, Glucose and insulin concentration patterns in cerebrospinal fluid following intravenous glucose injection in humans, Endokrinologie, 76: 185–188.

    PubMed  CAS  Google Scholar 

  • Roberts R.J., 1980, Directory of restriction endonucleases, in Methods in Enzymology v. 68, ed. R. Wu, Academic Press, NY, pp.

    Google Scholar 

  • Sanger F., Nicklen S. and Coulson A.R., 1977, DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Sara V.R., Hall K., Misaki M., Fry kl und L., Christensen N. and Wetterberg L., 1983, Ontogenesis of somatomedin and insulin receptors in the human fetus, J. Clin. Inves. 71: 1084–1094.

    Article  CAS  Google Scholar 

  • Sara V.R., Hall K., Holtz H.V., Humbel R., Sjögren B. and Wetterberg L., 1982, Evidence for the presence of specific receptors for insulin-like growth factors 1.(IGF-1) and 2(IGF-2) and insulin throughout the adult human brain, Neurosei.Letts. 34: 39–44.

    Article  CAS  Google Scholar 

  • Skaper S.D., Selak I. and Varon S., 1982, Molecular requirements for survival of cultured avian and rodent dorsal root ganglionic neurons responding to different trophic factors, J.Neuro sei. Res. 8: 251–261.

    Article  CAS  Google Scholar 

  • Song H-Y and Villa-Komaroff L., 1984, Isolation and characterization of a double-stranded cDNA clone encoding preproinsulin, Scientia Sinica, in press.

    Google Scholar 

  • Sures I., Goeddel D.V., Gray A. and Ullrich A., 1980, Nucleotide sequence of human preproinsulin complementary DNA. Science 208:57–59.

    Article  PubMed  CAS  Google Scholar 

  • Sutcliffe J.G., 1978, The complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp. 43:77–90.

    Article  Google Scholar 

  • Temin H. and Mizutani S., 1970, RNA-dependent DNA polymerase in virons of Rous sarcoma virus. Nature 226:1211–1213.

    Article  PubMed  CAS  Google Scholar 

  • Thomas P.S., 1980, Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose, Proc. Natl. Acad. Sci. USA 77: 5201–5205.

    Article  PubMed  CAS  Google Scholar 

  • Uvnäs B. and Uvnäs-Wallenstein K., 1978, Insulinergic nerves to the skeletal muscles of the cat? Acta Physiol. Scand. 103: 346–348.

    Google Scholar 

  • Uvnäs-Moberg K., Posioncee B., Hagerman M., Castensson S., Rubio C. and Uvnäs B., 1982, Occurence of an ihsulin-like peptide in extracts of peripheral nerves of the cat and in extracts of human vagal nerves. Acta Physiol. Scand. 115: 471–477.

    Article  PubMed  Google Scholar 

  • Uvnäs-Wallensten K., 1981, Peptides in metabolic autonomic nerves, Diabetologia 20: 337–342.

    Article  PubMed  Google Scholar 

  • van Houten M. and Posner B.I., 1979, Insulin binds to blood vessels invitro, Mature 282: 623–625.

    Article  Google Scholar 

  • van Houten M. and Posner B.I., 1981, Cellular basis of direct insulin action in the central nervous system, Diabetologie 20: 255–267.

    Article  Google Scholar 

  • van Houten M., Posner B.I., Kopriwa B.M. and Brawer J.R., 1980, Insulin binding sites localized to nerve terminals in rat median eminence and arcuate nucleus, Science 207: 1081–1083.

    Article  PubMed  Google Scholar 

  • van Houten M., Posner B.I., Kopriwa B.M. and Brawer J.R., 1979, Insulin binding sites in the rat brain: invivo localization to the circumventricular organs by quantitative radioautography, Endocrinology 105: 666–679.

    Article  PubMed  Google Scholar 

  • van Ness J., Maxwell I.H. and Hahn W.E., 1979, Complex population of nonpolyadenylated messenger RNA in mouse brain, Cell 18: 1341–1349.

    Article  PubMed  Google Scholar 

  • Villa-Komaroff L., 1980, The synthesis of eukaryotic proteins in prokaryotic cells, in Gene Structure and Expression, ed. D. H. Dean, L. F. Johnson, P. C. Kimball and P. S. Perlman, Ohio State University Press, Columbus, pp. 81–105.

    Google Scholar 

  • Villa-Komaroff L., Efstratiadis A., Broome S., Lomedico P., Tizard R., Naber S.P., Chick W.L. and Gilbert W., 1978, A bacterial clone synthesizing proinsulin. Proc. Nat. Acad. Sci. USA 75: 3727–3731.

    Article  PubMed  CAS  Google Scholar 

  • Wensink P.C., Finnegan D.J., Donelson J.E. and Hogness D.S., 1974, A system for mapping DNA sequences in the chromosomes of D. melanogaster. Cell 3: 315–325.

    Article  PubMed  CAS  Google Scholar 

  • Weyhenmeyer J.A. and Fellows R.E., 1983, Presence of immunoreactive insulin in neurons cultured from fetal rat brain, Cell Molec.Neuro. 3: 81–86.

    Article  CAS  Google Scholar 

  • Woods S.C. and Porte D. Jr., 1977, Relationship between plasma and cerebrospinal fluid insulin levels of dogs, Amer. J. Physiol. 233: E331–E334.

    PubMed  CAS  Google Scholar 

  • Woods S.C., Lotter E.C., McKay L.D., and Porte Jr., D., 1979, Chronic intracerebroventricular infusion of insulin reduces food intake and body weight of baboons, Nature 282: 503–505.

    Article  PubMed  CAS  Google Scholar 

  • Yallow R. and Eng J., 1983, Insulin in the central nervous system, Advances in Metabolic Disorders 10: 341–354.

    Google Scholar 

  • Yang J.W., Raizada M.K. and Fellows R.E., 1981, Effects of insulin on cultured rat brain cells: stimulation of ornithine decarboxylase activity, J. Neurochem. 36: 1050–1057.

    Article  PubMed  CAS  Google Scholar 

  • Young R.A. and Davis R.W., 1983, Efficient isolation of genes by using antibody probes, Proc. Natl. Acad. Sci. USA 80: 1194–1198.

    Article  PubMed  CAS  Google Scholar 

  • Zabeau, M., and Roberts, R.J., 1979, The role of restriction endonucleases in molecular genetics, in Molecular Genetics, ed. J.H. Taylor, Academic Press, NY. pp. 1–637

    Google Scholar 

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Authors and Affiliations

  1. Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, MA, 01605, USA

    Lydia Villa-Komaroff, Antonio Gonzalez, Song Hou-Yan, Bruce Wentworth & Paul Dobner

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  1. Lydia Villa-Komaroff
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  2. Antonio Gonzalez
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  3. Song Hou-Yan
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  4. Bruce Wentworth
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  5. Paul Dobner
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Editors and Affiliations

  1. University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

    Jean M. Lauder

  2. National Institutes of Health, Bethesda, Maryland, USA

    Philip G. Nelson

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© 1984 Plenum Press, New York

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Villa-Komaroff, L., Gonzalez, A., Hou-Yan, S., Wentworth, B., Dobner, P. (1984). Novel Insulin-Related Sequences in Fetal Brain. In: Lauder, J.M., Nelson, P.G. (eds) Gene Expression and Cell-Cell Interactions in the Developing Nervous System. Advances in Experimental Medicine and Biology, vol 181. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4868-9_7

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  • DOI: https://doi.org/10.1007/978-1-4684-4868-9_7

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