Abstract
Studies concerning the regulatory aspects of secondary metabolism in plant tissue and cell cultures have shown that very few systems can produce the characteristic plant products in significant yields (1). Although it has now been possible to isolate high yielding competent cell lines from a heterogeneous cell population (2, 3), the stability of such selected lines is rather poor. Our studies with cultured tissues of Tylophora indica and Atropa belladonna have shown that the process of organogenesis and cellular differentiation, brought about during the developmental stages of plant growth, have profound influence on the expression of secondary metabolism (4, 5). These observations suggested that in vitro culture of isolated plant organs may prove advantageous for the production of secondary products. In this report we present our results concerning the shoot organ cultures of Atropa belladonna and Rauwolfia serpentina.
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M.S. Chadha and M.R. Heble, Biotechnological applications of plant tissue and cell cultures: problems and prospects, in: “Golden Jubilee Commemoration Volume,” U.S. Srivastava, ed., National Academy of Sciences, Calcutta, India (1980).
M.R. Heble, Plant tissue culture, a source of natural products. Biosynthesis of steroids and naphthoquinones, in: “Cultivation and Utilization of Medicinal and Aromatic Plants,” C.K. Atal and B.M. Kapur, eds., Regional Research Laboratory, Jammu-Tawi (1977).
M.H. Zenk, The impact of plant cell culture on industry, in: “Frontiers of Plant Tissue Culture,” T.A. Thorpe, ed., International Association for Plant Tissue Culture (1978).
B.D. Benjamin, M.R. Heble and M.S. Chadha, Alkaloid synthesis in tissue cultures and regenerated plants of Tylophora indica Merr., Z. Pflanzenphysiol. 92:77–84 (1979).
S. Eapen, T.S. Rangan, M.S. Chadha and M.R. Heble, Biosynthetic and cytological studies in tissue cultures and regenerated plants of haploid Atropa belladonna, Can. J. Bot. 56:2781–2784 (1978).
H.N. Wood and A.C. Braun, Studies on the regulation of certain essential biosynthetic systems in normal and crown-gall tumor, Proc. Natl. Acad. Sci. USA 47:1907–1913 (1961).
T. Murashige and F. Skoog, A revised medium for rapid growth and bioassay with tobacco tissue culture, Physiol. Plant. 15:473–497 (1962).
M. Lin and E.G. Staba, Peppermint and spearmint tissue cultures. I. Callus formation and submerged culture, Lloydia 24:139–145 (1961).
S. Eapen, T.S. Rangan, M.S. Chadha and M.R. Heble, Morphogenetic and biosynthetic studies on tissue cultures of Atropa belladonna L., Plant Sci. Lett. 13:83–89 (1978).
A. Krishnamurthy, “The Wealth of India — Raw Materials, Vol. VIII,” Publications and Information Directorate, CSIR, New Delhi (1969).
G.C. Mitra, Effect of amino acids on growth of excised belladonna root cultures and on their atropine synthesis, in: “Plant Tissue Culture, Genetic Manipulation and Somatic Hybridization of Plant Cells,” P.S. Rao, M.R. Heble and M.S. Chadha, eds., BARC, Bombay (1980).
T. Murashige, The impact of plant tissue culture on agriculture, in: “Frontiers of Plant Tissue Culture,” T. A. Thorpe, ed., International Association for Plant Tissue Culture (1978).
M.R. Heble and E.J. Staba, Diosgenin synthesis in shoot cultures of Dioscorea composita, Planta Medica Supp.:120-123 (1980).
J.H.C. Lui and E.J. Staba, Effects of age and growth regulators on serially propagated Digitalis lanata leaf and root cultures, Planta Medica 41:90–95 (1981).
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© 1983 Springer Science+Business Media New York
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Heble, M.R., Benjamin, B.D., Roja, P.C., Chadha, M.S. (1983). Studies on Shoot Organ Cultures of Atropa Belladonna and Rauwolfia Serpentina . In: Sen, S.K., Giles, K.L. (eds) Plant Cell Culture in Crop Improvement. Basic Life Sciences, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4379-0_7
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DOI: https://doi.org/10.1007/978-1-4684-4379-0_7
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