Abstract
A morphogenically competent cell may be defined as one which has the potential or acquires the potential in vitro to develop as embryo, callus or other structure given appropriate cultural conditions. Most tissue culturists probably regard competence mainly as a property of culture and one that is acquired and regulated by the particular combination and concentration of hormones included in the medium. In this paper I propose to query this assumption and to put the case that (a) competence may be more readily induced before culture, and (b) expression of that competence is dependent as much upon endogenous as upon exogenous factors. Pollen will be used to illustrate this theme because of the ease with which competent cells can be recognized in suitably stained squash preparations of anthers. I shall refer mainly to the peonies and barley, since these are the two groups of plants in which I first encountered competent cells in vivo, a phenomenon subsequently referred to as pollen dimorphism (1).
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References
N. Sunderland, Anther culture as a means of haploid induction, in: “Haploids in Higher Plants: Advances and Potential,” K.J. Kasha, ed., University of Guelph Press (1974).
M.S. Ramanna, The origin and in vivo development of embryoids in the anthers of Solanum hybrids, Euphytica 23:623–632 (1974).
N. Sunderland, Observations on anther culture of ornamental plants, in: “La culture des tissus et des cellules des végétaux. Travaux dédiés à la mémoire de Georges Morel,” R.J. Gautheret, ed., Masson, Paris (1977).
Y.S. Murty and V. Kumar, In vivo production of plantlets from the anthers of Dianthus caryophyllus L., Acta Bot. Ind. 42:172–173 (1976).
N. Sunderland, Induction of growth in the culture of pollen, in: “Differentiation in vitro,” M.M. Yeoman, D.E.S Truman, eds., Cambridge University Press (1982).
N. Sunderland and F.M. Wicks, Embryoid formation in pollen grains of Nicotiana tabacum, J. Exp. Bot. 22:213–226 (1971).
N. Sunderland, G.B. Collins and J.M. Dunwell, The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill, Planta 117:227–241 (1974).
N. Sunderland and J.M. Dunwell, Pathways in pollen embryogenesis, in: “Tissue Culture and Plant Science 1974,” H.E. Street, ed., Academic Press, London (1974).
P.J. Dale, Pollen dimorphism and anther culture in barley, Planta 127:213–220 (1975).
M. Horner and H.E. Street, Pollen dimorphism — origin and significance in pollen plant formation by anther culture, Ann. Bot. 42:763–771 (1978).
N. Sunderland, Strategies in the improvement of yields in anther culture, in: “Proceedings of Symposium on Plant Tissue Culture,” Science Press, Peking (1978).
N. Sunderland, Anther and pollen culture 1974–1979, in: “The Plant Genome,” D.R. Davies and D.A. Hopwood, eds., John Innes Charity, Norwich (1980).
N. Sunderland and M. Roberts, Anther and pollen culture, in: “John Innes Annual Report #67,” Norwich (1976).
M. Roberts and N. Sunderland, Pollen culture in Paeonia, in: “John Innes Annual Report #68,” Norwich (1977).
C. Nitsch, Pollen culture — a new technique for mass production of haploid and homozygous plants, in: “Haploids in Higher Plants: Advances and Potential,” K.J. Kasha, ed., University of Guelph Press (1974).
E. Heberle-Bors and J. Reinert, Isolated pollen cultures and pollen dimorphism, Naturwiss 67:311–312 (1980).
W. Wernicke, C.T. Harms, H. Lorz and E. Thomas, Selective enrichment of embryogenic microspore populations, Naturwiss 65:540–541 (1978).
C. Nitsch and B. Norreel, Effet d’un choc thermique sur le pouvoir embyrogène de pollen de Datura innoxia cultivé dans l’anthère ou isolé de l’anthère, C R hebd Séanc Acad. Sci. Paris 276:303–306 (1973).
N. Sunderland and M. Roberts, Cold-pretreatment of excised flower buds in float culture of tobacco anthers, Ann. Bot. 43:405–414 (1979).
N. Sunderland and D.C. Wildon, A note on the pretreatment of excised flower buds in float culture of Hyoscyamus anthers, Plant Sci. Lett. 15:169–175 (1979).
N. Sunderland, Comparative studies of anther and pollen culture, in: “Plant Cell and Tissue Culture: Principles and Applications,” W.R. Sharp, P.O. Larsen, E.F. Paddock and V. Raghavan, Eds., State University Press, Columbus, Ohio (1979).
S.S. Bhojwani, J.M. Dunwell and N. Sunderland, Nucleic-acid and protein contents of embryogenic tobacco pollen, J. Exp. Bot. 24:863–871 (1973).
J.M. Dunwell and N. Sunderland, Pollen ultrastructure in anther culture of Nicotiana tabacum. II. Changes associated with embryogenesis, J. Exp. Bot. 25:363–373 (1974).
B. Huang and N. Sunderland, Temperature-stress pretreatment in barley anther culture, Ann. Bot. 49:77–88 (1982).
W.A. Keller and K.C. Armstrong, Stimulation of embryogenesis and haploid production in Brassica campestris anther culture by elevated temperature treatment, Theor. Appl. Genet. 55:65–67 (1979).
N. Sunderland and L.J. Evans, Multicellular pollen formation in cultured barley anthers. II. The A, B, and C pathways, J. Exp. Bot. 31:501–514 (1980).
N. Sunderland and M. Roberts, New approach to pollen culture, Nature 270:236–238 (1977).
Y. Chen, R.F. Wang, W.H. Tian, Q. Zuo, S. Zheng, D.Y. Lu and G. Zhang, Studies on pollen culture in vitro and induction of plantlets in Oryza sativa subsp. Keng., Acta Gen. Sinica 7:46–47 (1980).
Z.H. Xu and N. Sunderland, Pollen culture in Hordeum vulgare, J. Exp. Bot. (in press).
M. Horner and H.E. Street, Problems encountered in the culture of isolated pollen of a Burley cultivar of Nicotiana tabacum, J. Exp. Bot. 29:217–226 (1978).
A. Rashid and J. Reinert, High-frequency embryogenesis in ab initio pollen culture of Nicotiana tabacum, Naturwiss 68:378–379 (1981).
N. Sunderland, M. Roberts, L.J. Evans and D.C. Wildon, Multicellular pollen formation in cultured barley anthers. I. Independent division of the generative and vegetative cells, J. Exp. Bot. 30:1133–1144 (1979).
Z.H. Xu and N. Sunderland, Inoculation density in the culture of barley anthers, Scientia Sinic. (in press).
Z.H. Xu, B. Huang and N. Sunderland, Culture of barley anthers in conditioned media, J. Exp. Bot. 32:767–778 (1981).
Z.H. Xu and N. Sunderland, Glutamine, inositol and conditioning factor in the production of pollen callus in vitro, Plant Sci. Lett. 23:161–168 (1981).
T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant. 15:473–497 (1962).
C.C. Chu, The N6 medium and its application to anther culture of cereal crops, in: “Proceedings of Symposium on Plant Tissue Culture,” Science Press, Peking (1978).
C.C. Chuang, T.W. Ouyang, H. Chia, S.M. Chou and C.K. Ching, A set of potato media for wheat anther culture, in: “Proceedings of Symposium on Plant Tissue Culture,” Science Press, Peking (1978).
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Sunderland, N. (1983). The Concept of Morphogenic Competence with Reference to Anther and Pollen Culture. In: Sen, S.K., Giles, K.L. (eds) Plant Cell Culture in Crop Improvement. Basic Life Sciences, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4379-0_14
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DOI: https://doi.org/10.1007/978-1-4684-4379-0_14
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