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Radioenzymatic Analyses

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Experimental Neurochemistry

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Abstract

Since the late 1960s, radioenzymatic assays have been developed for many of the neurotransmitters and, in some cases, for some of their precursors and metabolites. These techniques have largely replaced the less sensitive and less specific spectrofluorometric and bioassay procedures used previously and now provide one of the more commonly used means to measure picogram amounts of these substances in very small volumes of body fluids, in individual nuclei taken from specific brain regions, and, in some cases, in large individual cells of some simple animals. This chapter reviews the development of radioenzymatic assays for substances commonly believed to act as neurotransmitters in the CNS, for their associated precursors and metabolites, and for some of the compounds, notably the trace amines, which may also play a role in neurotransmission. In addition, applications of these techniques to transmitter determinations in very small quantities of biological specimens and, in some cases, in single cells are discussed.

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  1. Psychiatric Research Division, University Hospital, Saskatoon, Saskatchewan, S7N OXO, Canada

    Stephen R. Philips

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  1. Center for Neurochemistry, Ward’s Island, New York, USA

    Abel Lajtha

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Philips, S.R. (1982). Radioenzymatic Analyses. In: Lajtha, A. (eds) Experimental Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4208-3_5

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