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Pathological implications of cell aging in vitro

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Biology of Aging and Development

Part of the book series: Faseb Monographs ((FASEBM,volume 3))

Abstract

The replicative capacity of cultured human fibroblasts is discussed in relation to three areas, diabetes mellitus, expression of HL-A antigens, and interactions with polymerizing fibrin. The replicative capacity of cells is diminished in diabetes mellitus and certain related disorders such as progeria and Werner’s syndrome, all of which feature accelerated aging. Expression of HL-A antigens is reduced in progeria fibroblasts compared to normal cultures at corresponding stages of passage. Normal cells show more subtle alteration during aging in vitro probably related to clonal heterogeneity and/or selection within mass cultures. Early-passage fibroblasts interact rapidly with polymerizing fibrin to form a mature clot which is then retracted by a process dependent on cellular integrity and active metabolism. Late-passage cultures are less active in both parameters as are fibroblasts from a subject with progeria. These observations, in total, may relate to altered self-recognition and certain autoimmune concomitants of aging in vivo. They may also help to explain impaired wound healing and increased predisposition to atherothrombosis in aging and diabetic individuals. This system of cultured human fibroblasts should serve as an excellent model to investigate the cellular and molecular basis of diabetes mellitus, aging and related pathology.—Goldstein, S., S. Niewiarowski and D. P. Singal. Pathological implications of cell aging in vitro. Federation Proc. 34: 56–63, 1975.

From Session III, Finite versus infinite proliferative and functional capacities of cells, of the FASEB Conference on Biology of Development and Aging, presented at the 58th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N. J., April 10, 1974.

Supported by grants from the Medical Research Council of Canada, the Canadian Diabetic Association Foundation Fund, and Hoechst Pharmaceuticals Limited of Canada and the U.S. during the tenure of a Medical Research Council Scholarship (S.G.).

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Abbreviations

MPD:

mean population doublings

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Authors and Affiliations

  1. Departments of Medicine and Pathology, McMaster University Medical Centre, Hamilton, Ontario, L8S 4J9, Canada

    S. Goldstein, S. Niewiarowski & D. P. Singal

  2. Specialized Center for Thrombosis Research, Temple University Health Sciences Center, Philadelphia, Pennsylvania, 19122, USA

    S. Goldstein, S. Niewiarowski & D. P. Singal

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  1. S. Goldstein
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  2. S. Niewiarowski
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  3. D. P. Singal
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  1. New York University School of Medicine, USA

    G. Jeanette Thorbecke

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© 1975 Federation of American Societies

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Goldstein, S., Niewiarowski, S., Singal, D.P. (1975). Pathological implications of cell aging in vitro. In: Thorbecke, G.J. (eds) Biology of Aging and Development. Faseb Monographs, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2631-1_12

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  • DOI: https://doi.org/10.1007/978-1-4684-2631-1_12

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