Abstract
DNA strand breaks, induced by exposure to chemicals or ionizing radiation stimulate poly(ADP-ribose) polymerase activity (1, 2). The resultant protein modifications have been postulated to comprise an important step in the DNA repair process (3). Inhibitors of the polymerase have been shown to sensitize human fibroblasts (4) and certain tumor cells (5) to ionizing radiation and to inhibit the repair of potentially lethal radiation injury (6, 7). That the response of the tumor cell lines vary, with some showing sensitivity to inhibitors of poly(ADP-ribosyl)ation and irradiation while others do not, suggested a need for detailed investigation of the ADP-ribosylation process in these tumor cell lines. In the present study we report the quantitative variations in protein-bound mono(ADP-ribose) levels as well as poly(ADP-ribose) polymerase activities and cellular NAD levels of various tumor cells. To this end, we also describe the development and characterization of polyclonal antisera to mono(ADP-ribose) and its potential use as a probe for studies of ADP-ribosylation.
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Abbreviations
- PBS-BT:
-
Phosphate-buffered saline, 0.5% BSA, 0.05% Tween-20
- DCC:
-
Dextran-coated charcoal
- 3AB:
-
3-Aininobenzamide
- BZ:
-
Benzamide
- AD:
-
Antigenic determinant
- RIA:
-
Radioimmunoassay
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© 1989 Springer-Verlag New York Inc.
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Prasad, S.C., Thraves, P.J., Boyle, J., Dritschilo, A. (1989). Heterogeneity of Polyclonal Antisera to ADP-Ribose and Their Use as Probes for ADP-Ribosylation in Human Tumor Cells. In: Jacobson, M.K., Jacobson, E.L. (eds) ADP-Ribose Transfer Reactions. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-8507-7_18
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DOI: https://doi.org/10.1007/978-1-4615-8507-7_18
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4615-8509-1
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