Abstract
The Prussian blue reaction is the one most widely used for the cytochemical demonstration of iron (Perls, 1867). Other methods use the Turnbull blue reaction (Schmeltzer, 1933; Pearse, 1960) and iron sulfide (Quincke, 1868). In the latter the iron is precipitated as its sulfide with sulfurated hydrogen and treated with silver to make the granules visible by light microscopy. Hausmann (1965) adapted the procedure for hematological purposes, but the disadvantage is that the test is not specific for iron.
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Bibliography
Bernát, I.: Az égési anaemia pathogenesise (The Pathogenesis of Anemia after Thermal Injury). Akadémiai Kiadó, Budapest 1971.
Bessis, M., Breton-Gorius, J.: Iron metabolism in the bone marrow as seen by electron microscopy: A critical review. Blood 19, 635 (1962).
Bothwell, T. H., Finch, C. A.: Iron Metabolism. Little, Brown and Co., Boston 1962.
Bowman, W. D.: Abnormal (ringed) sideroblasts in various hematologic and non-hematologic disorders. Blood 18, 662 (1961).
Brüschke, G.: Der Siderocyt. Akademie Verlag, Berlin 1962.
Cartwright, G. E., Deiss, A.: Sideroblasts, siderocytes, and sideroblastic anemias. New Engl. J. Med. 292, 185 (1975).
Crosby, W. H.: Siderocytes and the spleen. Blood 12, 165 (1957).
Dacie, J. V., Doniach, J.: Basophilic property of iron-containing granules in siderocytes. J. Path. Bact. 59, 684 (1947).
Doniach, J., Grüneberg, H., Pearson, J. E. G.: The occurrence of siderocytes in adult human blood. J. Path. Bact. 55, 23 (1943).
Douglas, A. S., Dacie, J. V.: The incidence and significance of iron containing granules in human erythrocytes and their precursors. J. clin. Path. 6, 307 (1953).
Ehrenstein, G., Lockner, D.: Der physiologische Erythrocytenabbau. Acta haemat. (Basel) 22, 129 (1959).
Grüneberg, H.: Siderocytes: a new kind of erythrocytes. Nature 148, 139 (1941).
Hausmann, K.: Cytochemische Differenzierung und Lokalisation locker gebundener Schwermetalle in Blut- und Knochenmarkzellen. X. Kongreß der Europäischen Gesellschaft für Hämatologie, Strassburg 1965.
Hayhoe, F. G. J., Quaglino, D.: Refractory sideroblastic anemia and erythremic myelosis. Possible relationship and cytochemical observations. Brit. J. Haemat. 6, 381 (1960).
Heilmeyer, L.: Die sideroachrestischen Anämien. Folia haemat. (Frankfurt) N. F. 6, 1 (1961a).
Heilmeyer, L.: Durch Eisenstoffwechselstörungen bedingte Anämien. Wien. klin. Wschr. 73, 181 (1961b).
Heilmeyer, L.: Morphologie, Funktion und Bedeutung der Sideroblasten. Wien. Z. inn. Med. 44, 1 (1963).
Heilmeyer, L., Heilmeyer, I.: Der Eisenstoffwechsel. In: Heilmeyer, L., Hittmair, A. (eds.): Handbuch der gesamten Hämatologie, Vol. 1/1. 2nd ed., Urban und Schwarzenberg, München-Berlin-Wien 1959.
Heilmeyer, L., Keller, W., Vivell, O., Betke, K., Wöhler, F., Keiderling, W.: Die kongenitale Atransferrinämie. Schweiz. med. Wschr. 91, 1203 (1961).
Heilmeyer, L., Merker, H., Wetzel, H. P., Klemen, D., Burmeister, P., Haas, R.: Atransferrinämie bei nephrotischem Syndrom. Dtsch. med. Wschr. 90, 1649 (1965).
Jung, F.: Das Schicksal toxisch veränderter roter Blutzellen in der Milz. Klin. Wschr. 36, 63 (1958).
Kaplan, E., Zuelzer, W. W., Mouriquand, C.: Sideroblasts: a study of stainable non-hemoglobin iron in marrow normoblasts. Blood 9, 203 (1954).
Lindner, E.: Elektronenmikroskopischer Nachweis von Schwermetallen, cit. Schiebler, T. H.: Histochemie der Mineralstoffe, Acta histochem. (Jena), Suppl. III, 98 (1963).
McFadzean, A. J. S., Davis, L. J.: Iron staining erythrocyte inclusions with special reference to acquired hemolytic anaemia. Glasg. med. J. 28, 237 (1947).
Merker, H.: Cytochemie der Blutzellen. In: Heilmeyer, L. (ed.): Blut und Blutkrankheiten. Springer, Berlin-Heidelberg-New York 1968.
Pappenheimer, A. M., Thompson, W. P., Parker, D. D., Smith, K. E.: Anemia associated with unidentified erythrocytic inclusions after splenectomy. Quart. J. Med. 14, 75 (1945).
Pearse, A. G. E.: Histochemistry. Little, Brown and Co., Boston 1960.
Perls, M.: Nachweis von Eisenoxyd in gewissen Pigmenten. Virchows Arch. path. Anat. 39, 42 (1867).
Quincke, H. J.: Über das Verhalten der Eisensalze im Tierkörper. Arch. Anat. Phys. 757 (1868).
Schmeltzer, W.: Der mikrochemische Nachweis von Eisen in Gewebselementen mittels Rhodanwasserstoffsäure und die Konservierung der Reaktion in Paraffmöl. Z. wiss. Mikr. 50, 99 (1933).
Weinfeld, A.: Storage iron in man. Acta med. scand. Suppl. 427 (1964).
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Bernát, I. (1983). Cytochemical Stains and Microscopy. In: Iron Metabolism. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7308-1_16
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