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Natural Fluorescence Imaging of Leukemic Cells for Studying Uptake and Retention of Anthracyclines

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Drug Resistance in Leukemia and Lymphoma III

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 457))

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Abstract

Cells exposed to light of appropriate wavelength show fluorescence emission in the UV-visible spectral range. This phenomenon, called natural fluorescence (NF), is related to cell compounds, i.e. proteins (1), nicotinic coenzymes (2), flavins (3), etc., engaged in structural and metabolic processes. Thus a correlation does exist between NF emission and morpho-functional state of cells.

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References

  1. Weynrib I. and Steiner R.F., The luminescence of aromatic aminoacids, in Excited states of proteins and nucleic acids, R. F. Steiner and I. Weynrib eds., pp. 227–318, MacMillan Press, London and Basingstoke, UK, 1971.

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  2. Kohen E., Hirschberg J.C., Kohen C., Wouters A., Pearson A., Salmon J.M. and Thorell B., Multichannel microspectrofluorometry for topographic and spectral analysis of NAD(P)H fluorescence in single living cells. Biochim. Biophys. Acta, 396:149–154, 19775.

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  3. Benson C., Meyer R. A., Zaruba M.E. and McKhann G. M., Cellular autofluorescence — Is it due to flavins?., J. Histochem. Cytochem., 27:44–48, 1979.

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  4. Monici, G. Agati, P. Mazzinghi, R. Pratesi, F. Fusi, P. A. Bernabei, I. Landini, P. Rossi Ferrini. Image analysis of cell natural fluorescence. Diagnostic applications in haematology. In: In: Biomedical Systems and Technologies, SPIE Vol. 2928, N. I. Croitoru, M. Frenz, T. A. King, R. Pratesi, A. M. Verga Scheggi, S. Seeger, O. S. Wolfbeis Eds., 180–187, Vienna, 1996.

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  5. Monici, P. A. Bernabei, F. Fusi, P. Rossi Ferrini, I. Landini e P. Mazzinghi. Natural fluorescence imaging for the discrimination of normal peripheral blood cells and leukemic ones. British J. of Haematology, 93(supplement 2): 344, 1996.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Istituto di Elettronica Quantistica-CNR, Florence, Italy

    M. Monici & P. Mazzinghi

  2. Dip. di Fisiopatologia Clinica, University of Florence, Italy

    F. Fusi

  3. Div. di Ematologia, Policlinico di Careggi, University of Florence, Italy

    A. Degli Innocenti o Nocentini, I. Landini, I. Banchelli, B. Bartolozzi, V. Santini & P. A. Bernabei

  4. Sezione A, INFM, Florence, Italy

    M. Monici & F. Fusi

Authors
  1. M. Monici
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  2. F. Fusi
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  3. P. Mazzinghi
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  4. A. Degli Innocenti o Nocentini
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  5. I. Landini
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  6. I. Banchelli
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  7. B. Bartolozzi
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  8. V. Santini
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  9. P. A. Bernabei
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Editor information

Editors and Affiliations

  1. University Hospital Vrije Universiteit, Amsterdam, The Netherlands

    G. J. L. Kaspers , R. Pieters  & A. J. P. Veerman ,  & 

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© 1999 Springer Science+Business Media New York

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Monici, M. et al. (1999). Natural Fluorescence Imaging of Leukemic Cells for Studying Uptake and Retention of Anthracyclines. In: Kaspers, G.J.L., Pieters, R., Veerman, A.J.P. (eds) Drug Resistance in Leukemia and Lymphoma III. Advances in Experimental Medicine and Biology, vol 457. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4811-9_11

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  • DOI: https://doi.org/10.1007/978-1-4615-4811-9_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7180-9

  • Online ISBN: 978-1-4615-4811-9

  • eBook Packages: Springer Book Archive

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